Angiostrongylus cantonensis and A. malaysiensis Broadly Overlap in Thailand, Lao PDR, Cambodia and Myanmar: A Molecular Survey of Larvae in Land Snails

Rodpai, Rutchanee; Intapan, Pewpan M.; Thanchomnang, Tongjit; Sanpool, Oranuch; Sadaow, Lakkhana; Laymanivong, Sakhone; Aung, Win Papa; Phosuk, Issarapong; Laummaunwai, Porntip; Maleewong, Wanchai

doi:10.1371/journal.pone.0161128

Cited by 45 publications

(99 citation statements)

References 19 publications

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“…Haplotypes from Rodpai et al. (2016), also from wild‐caught larval samples, fell into Clade II. Rodpai et al.…”

Section: Discussionmentioning

confidence: 99%

“…Rodpai et al. (2016) referred to their samples as A. malaysiensis in conjunction with the finding of two diverged CO 1 clades that corresponded to two 18S ribosomal DNA (rDNA) clades that had prior species‐designated reference sequences (see below). Eamsobhana, Yong, et al.…”

Section: Discussionmentioning

confidence: 99%

“…(2016) in which they obtained both CO 1 and 18S rDNA sequences from the same individuals. Rodpai et al.…”

Section: Discussionmentioning

confidence: 99%

“…Rodpai et al. (2016) found complete congruence between two CO 1 clades and two 18S clades where the two 18S clades had prior reference sequences designating them as A. cantonensis (GenBank AY295804; Carreno & Nadler, 2003) or A. malaysiensis (GenBank EF514914; Fontanilla & Wade, 2008). To facilitate future studies, we provided Tables S5 and S6, which provide sample information linking 18S haplotypes and CO 1 haplotypes as well as the alignment of the unique 18S haplotypes of A. malaysiensis and A. cantonensis in fasta format.…”

Section: Discussionmentioning

confidence: 99%

“…For example, ribosomal DNA sequences were used to assess metastrongylid nematode relationships (Carreno & Nadler, 2003) or to help survey larvae from mollusk intermediate hosts (Fontanilla & Wade, 2008; Qvarnstrom, Sullivan, Bishop, Hollingsworth, & da Silva, 2007; Qvarnstrom et al., 2010), and ~360‐bp region of the cytochrome c oxidase 1 ( CO 1) gene was used to assess relationships to other species of Angiostrongylus (Eamsobhana et al., 2010). To date, molecular systematic/phylogeographic studies on A. cantonensis have mainly used two mitochondrial (mtDNA) markers, CO 1 and cytochrome b ( CYTB ), where the focus has largely been descriptive in terms of reporting local patterns of haplotype variants (Aghazadeh et al., 2015; Dalton, Fenton, Cleveland, Elsmo, & Yabsley, 2017; Dusitsittipon, Criscione, Morand, Komalamisra, & Thaenkham, 2017; Dusitsittipon, Thaenkham, Watthanakulpanich, Adisakwattana, & Komalamisra, 2015; Eamsobhana, Song, et al., 2017; Eamsobhana, Yong, et al., 2017; Lv et al., 2012; Monte et al., 2012; Moreira et al., 2013; Nakaya et al., 2013; Okano et al., 2014; Rodpai et al., 2016; Simoes et al., 2011; Tokiwa et al., 2012, 2013; Vitta et al., 2016; Yong, Eamsobhana, Song, Prasartvit, & Lim, 2015; Yong, Song, Eamsobhana, Goh, & Lim, 2015; Yong, Song, Eamsobhana, & Lim, 2016). …”

Section: Introductionmentioning

confidence: 99%

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Hurdles in the evolutionary epidemiology of Angiostrongylus cantonensis: Pseudogenes, incongruence between taxonomy and DNA sequence variants, and cryptic lineages

Dusitsittipon

Criscione

Morand

et al. 2018

Evolutionary Applications

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Angiostrongylus cantonensis, the rat lungworm, is a zoonotic pathogen that is one of the leading causes of eosinophilic meningitis worldwide. This parasite is regarded as an emerging pathogen with a global range expansion out of southeastern Asia post‐WWII. To date, molecular systematic/phylogeographic studies on A. cantonensis have mainly used two mitochondrial (mtDNA) markers, cytochrome c oxidase 1 (CO1) and cytochrome b (CYTB), where the focus has largely been descriptive in terms of reporting local patterns of haplotype variants. In order to look for more global evolutionary patterns, we herein provide a collective phylogenetic assessment using the six available whole mtDNA genome samples that have been tagged as A. cantonensis, A. malaysiensis, or A. mackerrasae along with all other GenBank CO1 and CYTB partial sequences that carry these species identifiers. The results reveal three important complications that researchers will need to be aware of, or will need to resolve, prior to conducting future molecular evolutionary studies on A. cantonensis. These three problems are (i) incongruence between taxonomic identifications and mtDNA variants (haplotypes or whole mtDNA genome samples), (ii) the presence of a CYTB mtDNA pseudogene, and (iii) the need to verify A. mackerrasae as a species along with other possible cryptic lineages, of which there is suggestive evidence (i.e., A. cantonensis could be a species complex). We provided a discussion of how these complications are hurdles to our understanding of the global epidemiology of angiostrongyliasis. We call for future studies to be more explicit in morphological traits used for identifications (e.g., provide measurements). Moreover, it will be necessary to repeat prior morphological and life‐history studies while simultaneously using sequence data in order to assess possible associations between critical epidemiological data (e.g., biogeography, virulence/pathology, host species use) and specific lineages.

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“…Haplotypes from Rodpai et al. (2016), also from wild‐caught larval samples, fell into Clade II. Rodpai et al.…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

“…(2016) in which they obtained both CO 1 and 18S rDNA sequences from the same individuals. Rodpai et al.…”

Section: Discussionmentioning

confidence: 99%