Angiotensin‐1‐converting enzyme inhibition, antioxidant activity, and modulation of cerebral Na+/K+ ATPase by free phenolics of African locust bean (<scp><i>Parkia biglobosa</i></scp>)

Komolafe, Kayode; Akinmoladun, Afolabi Clement; Komolafe, Titilope R.; Olaleye, Mary Tolulope; Boligon, Aline Augusti; Akindahunsi, Akintunde Afolabi; Rocha, João Batista Teixeira da

doi:10.1002/hsr2.17

Cited by 9 publications

(6 citation statements)

References 34 publications

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Section: Resultsmentioning

confidence: 99%

“…The inhibitory activity of BNP peptide against ACEII was determined as IC 50 = 63 μM using HHL as substrate (Table 1), which is basically in line with the reported peptide activity on ACE (IC 50 = 46.9 μM). [47] The total binding energy (ΔG = À17.9 kcal/mol) of BNP peptide to ACEII was further decomposed into each peptide residue using a strategy described by Hou et al [48] As can be seen in Figure 1c, the energetic contribution of residues increases from the peptide's N-terminus to C-terminus, which was characterized by amino acid descriptors. [49] This is expected if considering that the BNP adopts its C-terminus to deeply root in ACEII active site, thus primarily responsible for the peptide binding, whereas its N-terminus is loosely packed against enzyme and partially exposed to solvent, thus conferring a marginal effect to the binding.…”

Section: Residue Importance Of Aceiiinhibitory Bnp Peptidementioning

confidence: 99%

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Rational truncation, mutation, and halogenation of bradykinin neuropeptides as potent ACEII inhibitors by integrating molecular dynamics simulations, quantum mechanics calculations, and in vitro assays

Chen

2022

J Chinese Chemical Soc

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Human angiotensin‐converting enzyme‐ii (ACEII) is involved in the brain renin‐angiotensin system and plays a key role in angiotensin metabolism and neural regulation. Here, we systematically investigated the intermolecular interaction of ACEII with its natural inhibitor bradykinin neuropeptide (BNP) at molecular level and found that the residue importance increases from peptide N‐ to C‐terminus; the four C‐terminal residues accumulatively account for ~70% total binding potency for BNP∆N to ACEII, which represent an N‐truncated tetrapeptide (BNP∆N) with comparable ACEII‐inhibitory activity with the full‐length BNP peptide. Mutagenesis analysis imparted that the mutation of BPPb∆N's two C‐terminal residues to phenylalanine (Phe) would largely impair its inhibitory activity, whereas such mutation on the two N‐terminal residues has only a moderate effect on the activity. The two N‐terminal Phe‐mutated tetrapeptides BNP∆N(I−2F) and BNP∆N(K−3F) were then used as templates and the ortho (o)‐, meta (m)‐, para (p)‐positions of the phenyl ring of their Phe residues were systematically substituted with different halogen types. The halogen substitution separately at the m‐position of BNP∆N(I−2F) Phe−2 residue and at the p‐position of BNP∆N(K−3F) Phe−3 residue can form a ternary halogen bonding system with ACEII His353/Lys368 residues and a binary bonding system with ACEII Glu403 residue, respectively. The [Br]Phe‐containing BNP∆N(I−2F‐mBr) and [I]Phe‐containing BNP∆N(K−3F‐pI) were determined to have high affinities for ACEII, which were improved considerably from their unsubstituted counterparts BNP∆N(I−2F) and BNP∆N(K−3F), respectively.

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Section: Resultsmentioning

confidence: 99%

Section: Residue Importance Of Aceiiinhibitory Bnp Peptidementioning

confidence: 99%

Rational truncation, mutation, and halogenation of bradykinin neuropeptides as potent ACEII inhibitors by integrating molecular dynamics simulations, quantum mechanics calculations, and in vitro assays

Chen

2022

J Chinese Chemical Soc

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“…Potential therapeutic mechanisms through which Parkia biglobosa exerts its effects include the augmentation of nitric oxide (NO) bioavailability and enhancement of endothelial function, both of which promote antioxidant and antiinflammatory activities. Additionally, the plant facilitates arterial vasodilation and regulates the renin-angiotensin-aldosterone system (RAAS) [28,33].…”

Section: Cardioprotective and Antihypertensive Propertiesmentioning

confidence: 99%

“…Angiotensin II, a powerful vasoconstrictive and hypertensive agent, is synthesized by the ACE. Parkia biglobosa leaf extract has been linked to ACE-inhibitory activity due to its active polyphenolic components, including catechins [28]. In one study, the hypotensive effect of green tea in diabetic Goto-Kakizaki (GK) rats was linked to the ACE-inhibitory effects of flavanol catechins such as epigallocatechin gallate (EGCg), epicatechin gallate (ECg), and epicatechin [44].…”

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confidence: 99%

Contemporary Insights into the Biological Mechanisms of Parkia biglobosa

Komolafe,

Olaleye,

Huang

et al. 2024

IJERPH

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For a long time, traditional medicine has relied on the use of medicinal plants and herbal products which have served as the basis for numerous pharmaceuticals. Parkia biglobosa (Jacq) R.Br.ex. G. Don., commonly called the African locust bean tree, is a perennial deciduous plant native to West Africa where it is highly esteemed for its nutritional and traditional medicinal benefits. Parkia biglobosa’s ethnomedicinal uses include microbial infections such as diarrhea and chronic diseases like hypertension and type 2 diabetes mellitus. This article presents the current understanding of the molecular mechanisms underlying Parkia biglobosa’s biological effects. An electronic database search was conducted using P. biglobosa and its synonyms as keywords in Scientific Electronic Library Online, ISI Web of Knowledge, PubMed, Scopus, Science Direct, and Google Scholar. Consistently, scientific research has confirmed the medicinal effects of the plant’s extracts and active phytochemicals, including antimicrobial, analgesic, antidiabetic, antihypertensive, hypolipidemic, and neuroprotective properties, among others. It highlights the contributions of identified innate phytochemicals and existing limitations to therapeutic applications, as well as the need for and prospects for further research. Advancing our understanding of the medicinal plant’s biological mechanisms and the contributions of the active phytochemicals would allow for more effective exploration of its vast pharmacological potential and facilitate clinical applications.

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“…According to Cavazos and Mejia [ 8 ], the anti-hypertensive activity of the bioactive peptides presents in cereals with hypotensive effects contribute to preventing cardiovascular diseases. Likewise, it has been discovered that the hydrolyzed proteins and phenolic compounds promote the regulation of oxidative stress and decrease the appearance of associated chronic diseases [ 9 , 10 ].…”

Section: Introductionmentioning

confidence: 99%

Cereals as a Source of Bioactive Compounds with Anti-Hypertensive Activity and Their Intake in Times of COVID-19

García-Castro

Román‐Gutiérrez

Castañeda‐Ovando

et al. 2022

Foods

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Cereals have phytochemical compounds that can diminish the incidence of chronic diseases such as hypertension. The angiotensin-converting enzyme 2 (ACE2) participates in the modulation of blood pressure and is the principal receptor of the virus SARS-CoV-2. The inhibitors of the angiotensin-converting enzyme (ACE) and the block receptors of angiotensin II regulate the expression of ACE2; thus, they could be useful in the treatment of patients infected with SARS-CoV-2. The inferior peptides from 1 to 3 kDa and the hydrophobic amino acids are the best candidates to inhibit ACE, and these compounds are present in rice, corn, wheat, oats, sorghum, and barley. In addition, the vitamins C and E, phenolic acids, and flavonoids present in cereals show a reduction in the oxidative stress involved in the pathogenesis of hypertension. The influence of ACE on hypertension and COVID-19 has turned into a primary point of control and treatment from the nutritional perspective. The objective of this work was to describe the inhibitory effect of the angiotensin-converting enzyme that the bioactive compounds present in cereals possess in order to lower blood pressure and how their consumption could be associated with reducing the virulence of COVID-19.

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Angiotensin‐1‐converting enzyme inhibition, antioxidant activity, and modulation of cerebral Na+/K+ ATPase by free phenolics of African locust bean (Parkia biglobosa)

Cited by 9 publications

References 34 publications

Rational truncation, mutation, and halogenation of bradykinin neuropeptides as potent ACEII inhibitors by integrating molecular dynamics simulations, quantum mechanics calculations, and in vitro assays

Rational truncation, mutation, and halogenation of bradykinin neuropeptides as potent ACEII inhibitors by integrating molecular dynamics simulations, quantum mechanics calculations, and in vitro assays

Contemporary Insights into the Biological Mechanisms of Parkia biglobosa

Cereals as a Source of Bioactive Compounds with Anti-Hypertensive Activity and Their Intake in Times of COVID-19

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