Angiotensin-I-converting enzyme inhibitory activity and bitterness of enzymatically-produced hydrolysates of shrimp (Pandalopsis dispar) processing byproducts investigated by Taguchi design

ACE Inhibitory and Antioxidant Activities of Collagen Hydrolysates from the Ribbon Jellyfish (Chrysaora sp.)

“…These diff erent actions of the same enzyme on diff erent substrates or diff erent enzymes on the same substrate (as in our study) indicate that along with DH and other parameters, the protein sequence of the substrate or the effectiveness of a protease on a certain substrate (i.e. substrate specifi city) is a very important factor (7). Therefore, it is crucial to select an appropriate protease to produce specifi c bioactive peptide sequences, and this choice depends on the raw material proteins and the expected bioactivity.…”

“…In studies of marine protein sources and corn gluten (7,32,33), Protamex was reported to be more eff ective than alcalase, whereas Alemán et al (26) found that they were equally eff ective. In some studies, trypsin was more eff ective in producing ACE inhibitors than alcalase (3), but in other cases alcalase was more eff ective (18,34).…”

“…Biologically active peptides have antioxidant and/or antihypertensive activities and also possess nutritive value; thus they may prove to be useful in the pharmaceutical and food industries (7). Bioactive peptides have been isolated from diff erent proteins, including soy (8), whey (9), and casein (10), and from various marine sources such as fi sh-derived proteins, e.g.…”

“…sardine, tuna, cod, bonito (3), and waste from fi sh processing facilities (2,11). More and more investigations are being conducted to identify potential sources of bioactive peptides (4,6,7). Although collagen peptides are assumed to exhibit high antiradical and antihypertensive activities due to their high content of hydrophobic amino acids (5,6,12), to date few studies have focused on collagen or gelatin hydrolysates as a source of these peptides.…”

“…Moreover, by controlling the enzymatic hydrolysis process, polypeptides of certain sizes can be obtained, and they can be modifi ed to improve their functional properties (6). The characteristics and bioactivity of protein hydrolysates depend on many factors, including type of protease, enzyme-to-substrate ratio, concentration of the substrate, incubation time, temperature, and pH (7). The eff ects of diff erent enzymes (e.g.…”