Animal board invited review: Germplasm technologies for use with poultry

Santiago-Moreno, J.; Blesbois, Élisabeth

doi:10.1016/j.animal.2022.100475

Cited by 15 publications

(12 citation statements)

References 45 publications

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“…Since the 21st century, global market demand for poultry meat has increased year by year from 71,293,729.64 tons in 2001 to 139,219,230.84 tons in 2022 ( FAO, 2024 ), which prompted the development of intensive rearing systems and AI technology in the poultry industry ( Campbell et al, 2019 ; Shaheen, et al, 2020 ). As early as the last half of 20th century, AI technology began using in the chicken industry to improve fertility and achieve breeding goals ( Bilcik et al, 2005 ; Santiago-Moreno and Blesbois, 2022 ). Habibullah et al (2015) pointed out that compared to natural mating, AI technology could produce more chicks per hen; and the actual mating capability of each rooster could be increased tenfold through AI technology ( Kharayat et al, 2016 ; Santiago-Moreno et al, 2016 ).…”

Section: Introductionmentioning

confidence: 99%

Genome sequencing of drake semen micobiome with correlation with their compositions, sources and potential mechanisms affecting semen quality

Hu,

Li,

Xin

et al. 2024

Poultry Science

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Section: Introductionmentioning

confidence: 99%

Genome sequencing of drake semen micobiome with correlation with their compositions, sources and potential mechanisms affecting semen quality

Hu,

Li,

Xin

et al. 2024

Poultry Science

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“…Cryopreservation of testicular tissue has recently emerged as a promising technique for preserving biodiversity and also genetically valuable males ( 2 ). This technique is particularly interesting when sperm collection is not possible, such as in prepubertal animals that die unexpectedly, strongly seasonal species who die in non-breeding season, males suffering from some pathology related to azoospermia and even in prepubertal patients who will receive gonadotoxic therapies after cancer detection ( 3 – 6 ). Despite the latest advances, the use of this technique still remains a challenge due to the complex structure of testes, the heterogeneous response of multiple germ cells to cryopreservation and the differences between species ( 4 , 7 , 8 ).…”

Section: Introductionmentioning

confidence: 99%

“…Testicular tissues contain somatic cells, such as Sertoli, Leydig, and myoid cells and also many types of germ cells, ranging from spermatogonia, spermatocytes, and spermatids to spermatozoa ( 4 ). Following cryopreservation, testicular fragments can be grafted into a suitable host or early germ cells, like spermatogonia, can be extracted from testicular tissues and in vitro culture to resume, in both cases, spermatogenesis and produce mature spermatozoa for assisted reproductive technologies ( 3 , 5 , 6 ).…”

Section: Introductionmentioning

confidence: 99%

DNA integrity and viability of testicular cells from diverse wild species after slow freezing or vitrification

Peris-Frau

Benito-Blanco²,

Martínez‐Nevado³

et al. 2023

Front. Vet. Sci.

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Introduction and objectiveCryopreservation of testicular tissues offers new possibilities to protect endangered species, genetically valuable individuals or even the fertility potential of prepubertal individuals who have died unexpectedly. However, the use of this technique still remains a challenge. In this study, slow freezing and vitrification of testicular tissue was investigated to find out which cryopreservation method could better preserve the viability and DNA integrity of testicular germ cells in diverse wild species.MethodsTestes were obtained post-mortem from 18 artiodactyls (wild boar, roe deer, dwarf goat, mhor gazelle, European mouflon, African forest buffalo, Malayan tapir, dorcas gazelle, Iberian ibex, gnu, red river hog), 5 primates (colobus monkey, capuchin monkey, mandrill), 8 carnivores (gray wolf, Persian leopard, binturong, European mink, American black bear, suricata), and 2 rodents (Patagonian mara). The testicles belonged to adult individuals and were cut into small pieces and cryopreserved by needle immersed vitrification or uncontrolled slow freezing using a passive cooling device. After warming or thawing, testicular tissues were enzymatically digested and two germ cell types were differentiated based on their morphology: rounded cells (spermatogonia, spermatocytes, and early spermatids) and elongated cells (elongated spermatids and spermatozoa). Cell viability was assessed by SYBR-14/propidium iodide while DNA fragmentation by TUNEL assay with fluorescence microscope.Results and discussionOur preliminary results revealed that our uncontrolled slow freezing method better preserved the viability and DNA integrity of elongated cells than vitrification. Such trend was observed in all species, being significant in artiodactyls, carnivores, and primates. Similarly, the viability and DNA integrity of rounded cells was also better maintained in primates by uncontrolled slow freezing, while in carnivores, vitrification by needle immersion showed better results in this type of cells. In artiodactyls and rodents both techniques preserved the viability of rounded cells in a similar manner, although the DNA integrity of these cells was greater after needle immersed vitrification in artiodactyls.ConclusionsIn conclusion, the effectiveness of each cryopreservation method is affected by the phylogenetic diversity between species and cell type.

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“…Furthermore, semen cryopreservation is a process used to preserve sperm cells. This technique offers great potential for preserving animal cells, conserving germplasm threatened with extinction, and supporting the artificial insemination (AI) program for wild and farm animals (Santiago-Moreno & Blesbois, 2022). According to Haugan et al (2007), the technique allows long-term storage of sperm in liquid nitrogen (-196 °C).…”

Section: Introductionmentioning

confidence: 99%

The Quality of Frozen Friesian Holstein Semen after Long-Term Storage

Bahmid¹,

Karja²,

Arifiantini³

2023

Trop. Anim. Sci. J.

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Semen cryopreservation is the long-term storage at very low temperatures in liquid nitrogen for future use. This study investigates the quality of frozen Friesian Holstein (FH) semen after long-term storage. Samples of FH semen stored for 25, 20, 15, 10, 5, and 1 years were collected from one of the national centers for artificial inseminations. Frozen semen was stored in containers with liquid nitrogen at -196 ᵒC in a room with a temperature of 20 ᵒC The variables used after thawing were sperm motility, viability, and abnormalities, as well as plasma membrane integrity (IPM) using computer-assisted sperm analysis (CASA), eosin-nigrosine staining, and hypoosmotic swelling (HOS) test, respectively. Data were analyzed by one-way ANOVA and expressed as mean ± SEM. The result showed no significant differences in sperm viability, abnormalities, and IPM. Furthermore, sperm motility was >40%, consistent with the Indonesian standard for frozen bovine semen. CASA analysis showed that all variables of the motility pattern have no significant difference, except linearity (LIN). The Lin of sperm was lower in frozen semen after one and five years than after 20 and 25 years of storage. The overall quality of semen after 25, 20, 15, 10, 5, and 1 years of storage met the standard and was suitable for artificial insemination.

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Animal board invited review: Germplasm technologies for use with poultry

Cited by 15 publications

References 45 publications

Genome sequencing of drake semen micobiome with correlation with their compositions, sources and potential mechanisms affecting semen quality

Genome sequencing of drake semen micobiome with correlation with their compositions, sources and potential mechanisms affecting semen quality

DNA integrity and viability of testicular cells from diverse wild species after slow freezing or vitrification

The Quality of Frozen Friesian Holstein Semen after Long-Term Storage

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