2023
DOI: 10.5398/tasj.2023.46.1.13
|View full text |Cite
|
Sign up to set email alerts
|

The Quality of Frozen Friesian Holstein Semen after Long-Term Storage

Abstract: Semen cryopreservation is the long-term storage at very low temperatures in liquid nitrogen for future use. This study investigates the quality of frozen Friesian Holstein (FH) semen after long-term storage. Samples of FH semen stored for 25, 20, 15, 10, 5, and 1 years were collected from one of the national centers for artificial inseminations. Frozen semen was stored in containers with liquid nitrogen at -196 ᵒC in a room with a temperature of 20 ᵒC The variables used after thawing were sperm motility, viabi… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
1
1

Citation Types

0
2
0

Year Published

2023
2023
2023
2023

Publication Types

Select...
2

Relationship

0
2

Authors

Journals

citations
Cited by 2 publications
(2 citation statements)
references
References 35 publications
0
2
0
Order By: Relevance
“…Thus, there were no significant factors during the production and storage phases, that could potentially negatively influence the quality of the frozen-thawed semen, and that both national AICs manage bulls, freezing, and container systems adequately. Bahmid et al [ 17 ] claimed that frozen semen samples that had been preserved for 25 years, met the Indonesian national standard for AI. Sperm motility was also found to be positively correlated with the fertility rate in this study (p<0.01).…”
Section: Discussionmentioning
confidence: 99%
“…Thus, there were no significant factors during the production and storage phases, that could potentially negatively influence the quality of the frozen-thawed semen, and that both national AICs manage bulls, freezing, and container systems adequately. Bahmid et al [ 17 ] claimed that frozen semen samples that had been preserved for 25 years, met the Indonesian national standard for AI. Sperm motility was also found to be positively correlated with the fertility rate in this study (p<0.01).…”
Section: Discussionmentioning
confidence: 99%
“…Briefly, the deep-frozen semen straws were first retrieved from a -196 °C LN2 container, immediately wiped with a paper towel, and placed in a thawing water bath (37 °C) for 30 seconds. Thawed semen was analyzed for sperm viability according to [24]. Spermatozoa kinetics (total and progressive motility, and straight-line velocity), mucus penetration, and hyperactivity were evaluated by using a computer-assisted sperm analysis (CASA) system [25].…”
Section: Post-cryopreserved Semen Quality Evaluationmentioning
confidence: 99%