Animal hydatid cyst genotypes as a potential substitute for human hydatid cyst as a source of antigen for diagnosis of zoonotichydatidosis

Ramadan, Reem M.; Khalifa, Marwa M.; El-Akkad, Dina M Hamdy; Abdel-Wahab, A.; El-Bahy, Mohamed M.

doi:10.1007/s12639-020-01309-2

Cited by 14 publications

(6 citation statements)

References 27 publications

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“…In addition, (Franssen et al, 2011) recorded a high number of muscles cysts, antibodies levels and immune response in rats experimentally infected with T. spiralis larvae as well as they reported that there was a positive correlation between the number of recovered muscles cysts of the infected rats and the level of serum antibody. However, there was no elevation in mean ELISA OD values in hosts slaughtered at 10 dpi and this is considered to be acceptable as (Bruschi et al, 2019;Ramadan et al, 2021) stated that the elevation in the level of anti-parasite Ab in sera could not be recorded before 2-3 weeks post-infection.…”

Section: Discussionmentioning

confidence: 97%

The Role of Ducks (Muscovy) and Catfish (Clarias Lazera) Meat in Transmitting Trichinella Spiralis Infection

Malak¹,

Abdel-Radi²

2021

WVJ

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Trichinellosis is a worldwide zoonotic food-borne disease that causes public health problems. The present study investigated the role of domestic ducks (Muscovy) and catfish (Clarias lazera) in transmitting Trichinella spiralis infection in Egypt. Ducks, fish, and rats were inoculated by high doses of Trichinella spiralis (T. Spiralis) larvae in naturally infected muscles of pigs and by free larvae extracted from artificial digestion. Both methods failed to develop an infection in ducks. No worms or cysts could be detected in ducks slaughtered 10, 25, and 45 days after inoculation. Moreover, there was no significant increase in the mean ELISA optical density (OD) value, compared to the control non-inoculated ducks. Inoculation of fish resulted in a significant increase in the mean ELISA OD value, compared to the control non-inoculated fish. This elevation was associated with the temporary detection of a few adult worms in the intestine of these fish which decreased with time till disappeared 45 days after inoculation but a non-significant increase was observed, compared to that obtained in the inoculated rats at the same stages. A large number of T. spiralis adult worms and cysts associated with a significant increase in the mean ELISA OD were recorded in the inoculated rats. Infection of rats (xenodiagnosis) by muscles collected from the inoculated ducks or fish failed to induce infection or elevation in the level of anti-T. spiralis antibodies (ATs-Ab) in their sera. In conclusion, Muscovy ducks and Clarias lazera could not be infected by T. spiralis in their muscles and the consumption of their meat could not transmit this infection to consumers in Egypt. However, proper cooking of meat is still necessary to avoid infection with other species.

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Section: Discussionmentioning

confidence: 97%

The Role of Ducks (Muscovy) and Catfish (Clarias Lazera) Meat in Transmitting Trichinella Spiralis Infection

Malak¹,

Abdel-Radi²

2021

WVJ

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“…Using SPSS 27 (IBM, NY, USA), The mean, maximum, and minimum values associated with every character, as well as the length/width ratios for the suckers and the body, were determined by a statistical description 26 , 27 .…”

Section: Methodsmentioning

confidence: 99%

The phylogenetic position and analysis of Renicola and Apharyngostrigea species isolated from Cattle Egret (Bubulcus ibis)

Salem,

Mahdy,

Shaalan

et al. 2023

Sci Rep

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Cattle Egret (Bubulcus ibis) is one of the most well-known herons in Egypt. It is called the friend of the farmer because it benefits farmers and helps them get rid of insects and worms. It acts as a reservoir for many diseases. Few researchers have discussed the significance of parasitic diseases that affect this wild bird and may lead to mortalities among the population especially the importance of vital organs such as kidneys. Therefore, this study aimed to spotlight parasitic infection-affected herons in Egypt and consider the risks to this beneficial bird. During this study, 23 Bubulcus ibis were captured after their death from Abou Rewash Giza Governorate, Egypt, during the period from February to September (2022). Renicola species (spp.) and Apharyngostrigea spp. are two important digenean parasites that were recovered from the kidneys, and small intestine of the heron Cattle Egret (Bubulcus ibis) with an infection rate of (17.2%) and (11.8%) respectively. Histopathological techniques were used to assess tissue alterations while light microscopy and molecular assays were used to assess the parasites. The parasites’ morphological and morphometrical characteristics, as well as polymerase chain reaction and sequencing assays (mitochondrial sections), were investigated for the first time in Egypt. These parasites were given in-depth illustrations and drawings. The distinctive qualities of the two species were discussed. As the first record from Egypt, the nucleotide sequences discovered in this work have been uploaded into the GenBank database (accession numbers: OR021986 and OQ955829). Microscopically, the renal blood vessels had vasculitis, necrosis, and other degenerative alterations. Further research analyzing the health of various heron spp. and environmental deterioration can help to close information gaps about the interactions between parasites, their hosts, and environmental health.

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“…The first part of this study used indirect ELISA to test the diagnostic specificity and sensitivity of these crude antigens in capturing anti-Cc-Ab in sera of surgically proven C. cerebralis infected sheep, control non-infected sheep, and sera collected from sheep infected by other parasites in order to identify the most diagnostic C. cerebralis antigen. CcF-Ag and CcS-Ag proved to have higher diagnostic sensitivity and specificity than CcW-Ag (Huang et al, 2016;Mahdy et al, 207;Ramadan et al, 2021). In contrast, CcS-Ag proved to have higher diagnostic specificity than the other two antigens.…”

Section: Efficacy Of Indirect Dot-elisa and Eitb For The Diagnosis Of...mentioning

confidence: 98%

“…ELISA specificity is usually affected by the degree of purity and specificity of the antigens (Sabry, 2007;Taher et al, 2017). EITB technique is considered a highly specific diagnostic technique, but it is unsuitable for field applications (Mahdy et al, 2017;Ramadan et al, 2021). The identification of specific diagnostic protein fractions then elution and concentration of this fraction to use in modified ELISA technique after dotting a minute amount of this purified antigen on NC sheet or even in micro-ELISA technique introduce more specific diagnostic assay described as dot-ELISA.…”

Section: Efficacy Of Indirect Dot-elisa and Eitb For The Diagnosis Of...mentioning

confidence: 99%

“…They described fluid antigens of the bladder worms as specific and high protein content than other antigens. The selection of scolices antigen in this field was supported by Zhang et al (2012) and Ramadan et al (2021). As they mentioned, fluid antigens of metacestodes usually contain some proteins from the A B hosts, such as IgG, that might interfere with the accuracy of diagnosis because it cross-reacted with the specific immunoglobulin present in infected host sera.…”

Section: Efficacy Of Indirect Dot-elisa and Eitb For The Diagnosis Of...mentioning

confidence: 99%

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Improved Dot-ELISA Assay Using Purified Sheep Coenurus cerebralis Antigenic Fractions for the Diagnosis of Zoonotic Coenurosis

Ramadan¹,

El-Hafez²,

El-Bahy³

et al. 2022

WVJ

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Clinicians face significant problems in the diagnosis of zoonotic coenurosis. The current study aimed to develop an improved dot-Enzyme-linked-immunosorbent assay (dot-ELISA) for the diagnosis of zoonotic coenurosis using sheep Coenurus cerebralis scolices purified antigen (CcS-Ag) and to compare the obtained results with those of indirect ELISA and Enzyme-linked immunoelectrotransfer blot technique (EITB). Sera were collected from humans and sheep infected or suspected of infection with Coenurus cerebralis, control cases, and cases infected with other parasites. The CcS-Ag was proved to be the most specific antigen. This antigen was fractionated, and its specific polypeptides against anti-C. cerebralis antibodies (ACc-Ab) were identified using EITB. Fractions at the molecular weight (MW) of 48 and 58 kDa were proved as the only specific ones, eluted from the gel and concentrated, then dotted on the NC sheet as pooled antigen before its evaluation in the diagnosis of infection using dot-ELISA. Dot-ELISA demonstrated absolute 100% sensitivity and 100% specificity as recorded by EITB, compared to both fractions on a nitrocellulose (NC) sheet using surgically proved infected human or sheep sera as a gold standard. Diagnosis by ELISA using crude CcS-Ag revealed similar sensitivity but lower specificity (75%). The diagnostic accuracy of dot-ELISA was proved by comparing its results with postmortem data obtained post slaughtering of 20 suspected sheep and patients investigated by computed tomography (CT) and magnetic resonance imaging (MRI). In conclusion, the selection of specific fractions after EITB to be used in dot-ELISA improved the diagnostic value of the test as a diagnostic tool gathering the benefits of ELISA and EITB.

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Animal hydatid cyst genotypes as a potential substitute for human hydatid cyst as a source of antigen for diagnosis of zoonotichydatidosis

Cited by 14 publications

References 27 publications

The Role of Ducks (Muscovy) and Catfish (Clarias Lazera) Meat in Transmitting Trichinella Spiralis Infection

The Role of Ducks (Muscovy) and Catfish (Clarias Lazera) Meat in Transmitting Trichinella Spiralis Infection

The phylogenetic position and analysis of Renicola and Apharyngostrigea species isolated from Cattle Egret (Bubulcus ibis)

Improved Dot-ELISA Assay Using Purified Sheep Coenurus cerebralis Antigenic Fractions for the Diagnosis of Zoonotic Coenurosis

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