Animal model of chronic kidney disease using a unilateral technique of renal ischemia and reperfusion in White New Zealand rabbits

Togoe, Eliane Barbosa; Silva, Iandara Schettert; Cury, Juliana L.; Souza, Albert Schiaveto de; Borges, J. C.; Saturnino, Klaus Casaro

doi:10.1590/s0102-8650201400160005

Cited by 12 publications

(11 citation statements)

References 15 publications

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“…Bazzano et al 5 observed that 10-min unilateral renal IR in rats worsened urinary parameters (protein, urea, glucose, fractional excretion of potassium and urea, creatinine clearance, and activity of alkaline phosphatase and gamma-glutamyl transferase) 21 days after injury. Togoe et al 12 found that 20-min unilateral renal IR in rabbits led to histopathological lesions of the renal tissue and increased serum creatinine 80 days after injury. The creatine results in our study are consistent with those reported by Bazzano et al 5 and demonstrate that IR episodes, even for a few minutes, damage the kidneys and result in progressive RD.…”

Section: Discussionmentioning

confidence: 99%

Muscle changes with high-intensity aerobic training in an animal model of renal disease

Togoe

Silva²,

Cury

et al. 2019

Acta Cir. Bras.

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Purpose: To analyze the muscle changes with high-intensity aerobic training (HIAT) in an animal model of renal disease (RD). Methods: Twenty one adult Wistar rats were divided into 3 groups: healthy sedentary (HS), RD sedentary (RDS), RD aerobic training (RDAT). RDS and RDAT were subjected to unilateral renal ischemia-reperfusion (10 min) and 21days after that, RDAT was subjected to 6 weeks HIAT (swimming). Serum creatinine (Cr) and muscle morphometry (cross-sectional area = CSA) of gastrocnemius were analyzed. Results: Cr was higher (p = 0.0053) in RDS (0.82 ± 0.04) than in the others (RDAT 0.55 ± 0.04; HS 0.55 ± 0.04). Morphometric analysis (class interval of CSA in μm 2 /absolute frequency of muscle fibers in each class) indicated that 50th percentile occurred in: HS 7th class (3000.00-3499.00/515), RDS, 8th class (3500.00-3999.00/484), RDAT 5th class (2000.00-2499.00/856). CSA of largest fibers in RDS, RDAT, HS was 9953.00 μm 2 , 9969.00 μm 2 ,11228.00 μm 2 , respectively. High frequency of fibers with lower CSA occurred in 4th, 5th, 6th and 7th class in RDA, absence of fibers into 22nd, 23rd classes (RDS and RDAT). Conclusion: HIAT in an animal model of RD resulted in increased the number of muscle fibers with smaller CSA.

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Section: Discussionmentioning

confidence: 99%

Muscle changes with high-intensity aerobic training in an animal model of renal disease

Togoe

Silva²,

Cury

et al. 2019

Acta Cir. Bras.

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“…Previous studies have demonstrated that an appropriate experimental animal model was essential for the pharmacological and pathological investigations of human diseases. Adenine is one of the most frequently used drugs for establishing CRF because, at large doses, it induces abnormalities in uric acid metabolism, resulting in abnormal increase in Cr and BUN, and inflammatory reactions which resemble those that occur in humans [21]. Currently, increasing evidence have demonstrated that polysaccharides derived from natural herbal medicines or foods are reliable and safe agents for treating some stubborn diseases via regulation of immunity [22,23].…”

Section: Discussionmentioning

confidence: 99%

Protective effect of crude polysaccharide from Pao-TianXiong derived from monkshood, against chronic renal failure in mice

Wang¹,

Fan²,

Geng³

et al. 2021

Trop. J. Pharm Res

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Purpose: To investigate the effect of crude polysaccharide isolated from pao-tian-xiong on chronic renal failure in mice, and its monosaccharide composition. Methods: Male Kunming mice were orally treated with adenine (211.5 mg/kg/day) for 7 days, followed by either crude polysaccharides (125, 250 or 500 mg/kg), or positive drug solution (jinguishenqi pill, 2000 mg/kg) for another 7 days (each group had 15 mice). Mice in normal and negative control groups were given saline. Mental and physical states, blood urine nitrogen (BUN) and serum creatinine (SCr), kidney morphological changes and organ indices were determined. Histopathological examination of spleen and kidney tissues was also performed. The monosaccharide composition of crude polysaccharide was determined by high-performance liquid chromatography (HPLC) and gas chromatography and mass spectrometry (GC-MS). Results: Compared with negative control group, serum BUN (6.71 mmol/L vs. 8.61 mmol/L) and Cr (107.74 vs. 113.39 μmol/L) were significantly decreased by the crude polysaccharide isolate (p < 0.05), whereas epididymis index (0.2556 vs. 0.2135 %) and seminal vesicle index (0.5547 vs. 0.3945 %) were increased (p < 0.05). Histopathological examination showed that injuries to kidney, spleen, testis and epididymis decreased significantly. The crude polysaccharides contained mainly glucose, rhamnose, arabinose, galactose, mannose, galacturonic acid, glucuronic acid and xylose, and their contents ranged from 0.7 to 65 %. Conclusion: These results suggest that the crude polysaccharides of Pao-tian-xiong ameliorates CRF symptoms in mice, thereby providing experimental evidence in support of its use as an anti-CRF drug.

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“…• Renal ischemia and reperfusion, by aseptic surgery [11][12][13] ; • Kidney damage was monitored for 14 days, using urine dipstick tests, in which renal damage was verified by the parameters pH, density, urobilinogen, and urinary glucose, as well as the presence of proteinuria and leukocytes. Renal damage was monitored for 14 days, using a urine reagent strip, by semiquantitative determination of 10 parameters by chemical reaction, in which the kidney was selected by the parameters pH, density, urobilinogen, and urinary glucose, in addition to the presence of proteinuria and leukocytes; • After 14 days of surgery for induction of kidney disease, the animals comprising the diseased groups were injected intraperitoneally (i.p.)…”

Section: Diabetes-associated Kidney Diseasementioning

confidence: 99%

Effect of electrophysical resources on healing of neurotendinous injury in an experimental model of type I diabetes and kidney disease

Silva

Facco

et al. 2022

Acta Cir. Bras.

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To evaluate and describe the effect of electrophysical resources laser therapy (LLLT), intravascular laser blood irradiation (ILIB), and cryotherapy on the healing process of neurotendinous injury, as well as possible systemic changes, in the experimental model of type 1 diabetes associated with kidney injury. Methods: The animals were randomized into four groups: G1) healthy control with untreated injury; G2) healthy control with injury and treatment; G3) disease control with untreated lesion; G4) disease with injury and treatment. Furthermore, the treated groups were divided into three, according to the type of treatment. All animals were induced to neurotendinous injury and treated according to the therapeutic protocols. Healing and inflammation were analyzed by semiquantitative histopathological study. Results: It was observed in sick animals treated with cryotherapy and ILIB reduction of inflammatory exudate, presence of fibroblasts and organization of collagen, when compared to the effects of LLLT. Moreover, there was reduction in glycemic levels in the group treated with ILIB. Conclusions: Cryotherapy promoted reduction in inflammatory exudate and organization of collagen fibers, in addition to the absence of signs of tissue necrosis, in the groups treated with and without the disease. ILIB therapy showed the same findings associated with significant reduction in glycemic levels in the group of diseased animals. The application of LLLT showed increased inflammatory exudate, low organization of collagen fibers and low sign of tissue degeneration and necrosis.This study in a model of associated diseases (diabetes and kidney disease) whose effects of electrophysical resources studied after neurotendinous injury allows us to verify histopathological variables suggestive of patients with the same comorbidities.

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Animal model of chronic kidney disease using a unilateral technique of renal ischemia and reperfusion in White New Zealand rabbits

Cited by 12 publications

References 15 publications

Muscle changes with high-intensity aerobic training in an animal model of renal disease

Muscle changes with high-intensity aerobic training in an animal model of renal disease

Protective effect of crude polysaccharide from Pao-TianXiong derived from monkshood, against chronic renal failure in mice

Effect of electrophysical resources on healing of neurotendinous injury in an experimental model of type I diabetes and kidney disease

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