Animal models for the study of influenza pathogenesis and therapy

Barnard, Dale L.

doi:10.1016/j.antiviral.2008.12.014

Cited by 151 publications

(134 citation statements)

References 119 publications

(160 reference statements)

Supporting

Mentioning

133

Contrasting

Order By: Relevance

“…For these reasons, the use of rhesus macaques in influenza studies is limited and inappropriate. 26,27 In contrast, mice, particularly BALB/c mice, are currently intensively employed in studies of disease pathogenesis and vaccine efficacy. Influenza viruses rarely cause such symptoms as respiratory tract disease in mice, and these viruses must be adapted to replicate to high titers in mice.…”

Section: Discussionmentioning

confidence: 99%

Response of BALB/c mice to a monovalent influenza A (H1N1) 2009 split vaccine

Yang

Tang

et al. 2010

Cell Mol Immunol

View full text Add to dashboard Cite

The novel influenza A (H1N1) 2009 virus has emerged to cause the first pandemic of the twenty-first century. Disease outbreaks caused by the influenza A (H1N1) virus have prompted concerns about the potential for a pandemic and have driven the development of vaccines against this subtype of influenza A. In this study, we developed a monovalent influenza A (H1N1) split vaccine and evaluated its effects in BALB/c mice. Mice were immunized subcutaneously with 2 doses of the vaccine containing hemagglutinin (HA) alone or HA plus an aluminum hydroxide (Al(OH) 3 ) adjuvant. Immunization with varying doses of HA (3.75, 7.5,15,30, 45 or 60 mg) was performed to induce the production of neutralizing antibodies. The vaccine elicited strong hemagglutination inhibition (HI) and microneutralization, and addition of the adjuvant augmented the antibody response. A preliminary safety evaluation showed that the vaccine was not toxic at large doses (0.5 ml containing 60 mg HA1600 mg Al(OH) 3 or 60 mg HA). Moreover, the vaccine was found to be safe at a dose of 120 mg HA11200 mg Al(OH) 3 or 120 mg HA in 1.0 ml in rats. In conclusion, the present study provides support for the clinical evaluation of influenza A (H1N1) vaccination as a public health intervention to mitigate a possible pandemic. Additionally, our findings support the further evaluation of the vaccine used in this study in primates or humans.

show abstract

Section: Discussionmentioning

confidence: 99%

Response of BALB/c mice to a monovalent influenza A (H1N1) 2009 split vaccine

Yang

Tang

et al. 2010

Cell Mol Immunol

View full text Add to dashboard Cite

show abstract

“…In the mouse model, however, the major symptom parameters are weight loss and virus in the lungs [29]. We showed above that RG extract contains components inhibiting infl uenza A virus replication in vitro, and that orally administered RG extract not only ameliorates weight loss but also decreases death in infl uenza virusinfected mice.…”

Section: Discussionmentioning

confidence: 91%

Effect of Oral Administration of Korean Red Ginseng on Influenza A (H1N1) Virus Infection

Kim¹,

Kim²,

Kim

2011

Journal of Ginseng Research

View full text Add to dashboard Cite

Korean red ginseng (RG), which is a ginseng treated by heating and steaming, has biological activity similar to Panax ginseng. The effect of ginseng on influenza infection has not been studied although it is known to have a broad range of biological activities. The aim of the study is to investigate the effect of RG extract on infl uenza A (H1N1) virus infection. We investigated the inhibitory effect of RG extract on plaque formation by infl uenza A virus in a cell-based plaque assay, and the effect of orally administered RG on infl uenza A virus infection in mice. RG extract, which was applied at a non-cytotoxic concentration, inhibited plaque formation by infl uenza A virus in the cell-based plaque assay. The orally administered RG extract ameliorated body weight loss and signifi cantly increased survival in mice infected with infl uenza A virus. Our results suggest that RG extract has components that reduce the severity of infection by infl uenza A virus and could potentially be used as a complement to treatment of infl uenza A virus infections.

show abstract

“…Besides lungs, A/H1N1pdm virus was undetectable in the spleen, kidneys, brain, liver and colon, which indicates that A/H1N1pdm virus does not spread to extrapulmonary organs in mice [45] . Ferrets have also been widely accepted as a suitable laboratory animal model for influenza virus research, particularly on pathogenicity and transmissibility [43] . Morbidity and lung viral titers are higher in ferrets infected with A/H1N1pdm virus as compared with those infected with the seasonal influenza H1N1 virus [46,47] .…”

Section: Influenza Pathogenesis In Experimental Animal Modelsmentioning

confidence: 99%

“…Animal models, particularly mice, have proven to be a useful tool for the study of influenza viruses, due to its utility in measuring infectivity and pathogenesis, and subsequent application for the evaluation of vaccines and antiviral compounds [43] . Mice are not a natural host for influenza viruses, therefore, adaptation of human strains to murine lung is generally required.…”

Section: Influenza Pathogenesis In Experimental Animal Modelsmentioning

confidence: 99%

Swine-origin influenza-virus-induced acute lung injury: Novel or classical pathogenesis?

Maeda

Uede

2010

WJBC

View full text Add to dashboard Cite

Influenza viruses are common respiratory pathogens in humans and can cause serious infection that leads to the development of pneumonia. Due to their hostrange diversity, genetic and antigenic diversity, and potential to reassort genetically in vivo , influenza A viruses are continual sources of novel influenza strains that lead to the emergence of periodic epidemics and outbreaks in humans. Thus, newly emerging viral diseases are always major threats to public health. In March 2009, a novel influenza virus suddenly emerged and caused a worldwide pandemic. The novel pandemic influenza virus was genetically and antigenically distinct from previous seasonal human influenza A/H1N1 viruses; it was identified to have originated from pigs, and further genetic analysis revealed it as a subtype of A/H1N1, thus later called a swine-origin influenza virus A/H1N1. Since the novel virus emerged, epidemiological surveys and research on experimental animal models have been conducted, and characteristics of the novel influenza virus have been determined but the exact mechanisms of pulmonary pathogenesis remain to be elucidated. In this editorial, we summarize and discuss the recent pandemic caused by the novel swine-origin influenza virus A/H1N1 with a focus on the mechanism of pathogenesis to obtain an insight into potential therapeutic strategies.

show abstract

Animal models for the study of influenza pathogenesis and therapy

Cited by 151 publications

References 119 publications

Response of BALB/c mice to a monovalent influenza A (H1N1) 2009 split vaccine

Response of BALB/c mice to a monovalent influenza A (H1N1) 2009 split vaccine

Effect of Oral Administration of Korean Red Ginseng on Influenza A (H1N1) Virus Infection

Swine-origin influenza-virus-induced acute lung injury: Novel or classical pathogenesis?

Contact Info

Product

Resources

About