Animal Models of Burn Wound Management

Chang, Shu–Jen; Sartika, Dewi; Fan, Gang-Yi; Cherng, Juin–Hong; Wang, Yiwen

doi:10.5772/intechopen.89188

Cited by 7 publications

(2 citation statements)

References 53 publications

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“…Thus, the anti-inflammatory activity of the plant extract could contribute to its wound healing activity by inhibiting the formation and release of proinflammatory cytokines since overproduction of proinflammatory cytokines can worsen and prolong the inflammation phase. The inhibition of COX further inhibits the formation of PGE2 which reduces the pain sensation threshold that improves the feeding behavior and general well-being of the test subject and finally contributes to the rapid healing [ 48 ].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Wound Healing and Anti‐Inflammatory Activities of 80% Methanol Crude Extract and Solvent Fractions of Trichilia dregeana Sond (Meliaceae) Leaves in Mice

Shewaye

Kahaliw

Belete

et al. 2023

Evidence-Based Complementary and Alternative Medicine

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Introduction. The leaves of Trichilia dregeana Sond are traditionally used to treat wounds. Even though there have been in vitro studies and claims supporting wound healing, there are no scientific data on in vivo wound healing and anti-inflammatory activities of the leaves of T. dregeana. Objective. This study aimed to evaluate wound healing and anti-inflammatory activity of 80% methanol crude extract and solvent fractions of T. dregeana in mice. Method. The leaves of T. dregeana were dried, ground, and macerated with 80% methanol three times successively. The crude extract was fractioned by water, ethyl acetate, and hexane separately. The acute dermal and oral toxicity tests were done by applying 2000 mg/kg of 10% (w/w) crude extract ointment (CEO) topically and 2000 mg/kg of crude extract orally, respectively. The wound healing activity of the crude extract was evaluated on excision, incision, and burn wound models while the fractions were evaluated only on excision wound model. The anti-inflammatory activity of the crude extract was evaluated on xylene-induced ear edema and cotton pellet granuloma tests. Result. Both acute dermal and oral toxicity tests were found to be safe after topical application of 2000 mg/kg of 10% (w/w) CEO and oral administration of 2000 mg/kg of crude extract suspension, respectively. Both 5% and 10% (w/w) CEO produced significant ( p < 0.001) wound contraction and period of epithelialization from day 4 onwards as compared to simple ointment (SO) on both excision and burn wounds. The tensile strength was increased significantly ( p < 0.001) for the CEO-treated mice as compared to both untreated and SO groups. The crude extract also showed anti-inflammatory activity at 100, 200, and 400 mg/kg by inhibiting ear edema, exudate, and granuloma formation as compared to the SO group. Conclusion. The increase in wound contraction, reduction in period of epithelialization, and increase in tensile strength support the traditional claims of T. dregeana for wound healing.

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Section: Discussionmentioning

confidence: 99%

Evaluation of Wound Healing and Anti‐Inflammatory Activities of 80% Methanol Crude Extract and Solvent Fractions of Trichilia dregeana Sond (Meliaceae) Leaves in Mice

Shewaye

Kahaliw

Belete

et al. 2023

Evidence-Based Complementary and Alternative Medicine

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“…The animals were maintained under a standard laboratory-controlled environment with free access to food and water and kept in the same conditions for at least three days before the experiment. The hot water method was used to produce burn injury (Chang et al., 2020 ). A total of 15 rats were divided into five groups with three rats in each group.…”

Section: Methodsmentioning

confidence: 99%

Nanophyto-gel against multi-drug resistant Pseudomonas aeruginosa burn wound infection

2021

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Burn wound is usually associated by antibiotic-resistant Pseudomonas aeruginosa infection that worsens and complicates its management. An effective approach is to use natural antibiotics such as cinnamon oil as a powerful alternative. This study aims to investigate topical nanostructured lipid carrier (NLC) gel loaded cinnamon oil for Pseudomonas aeruginosa wound infection. A 2 4 full factorial design was performed to optimize the formulation with particle size 108.48 ± 6.35 nm, zeta potential −37.36 ± 4.01 mV, and EE% 95.39 ± 0.82%. FTIR analysis revealed no excipient interaction. Poloxamer 407 in a concentration 20% w/w NLC gel was prepared for topical application. Drug release exhibited an initial burst release in the first five hours, followed by a slow, sustained release of up to five days. NLC-cinnamon gel has a significant ability to control the drug release with the lowest minimum inhibitory concentration again P. aeruginosa compared to other formulations ( p < .05). In vivo study also showed NLC-cinnamon gel effectively healed the infected burned wound after a six-day treatment course with better antibacterial efficacy in burned animal models. Histological examination ensured the tolerability of NLC-cinnamon gel. The results suggest that nanoparticle-based cinnamon oil gel is a promising natural product against antibiotic-resistant strains of P. aeruginosa in wound infection.

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Evaluating the Effectiveness of Using Low-Level Laser Therapy for the Healing Process of Third-Degree Burns in Mice

Do,

Le,

Vong

et al. 2023

IFMBE Proceedings

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Animal Models of Burn Wound Management

Cited by 7 publications

References 53 publications

Evaluation of Wound Healing and Anti‐Inflammatory Activities of 80% Methanol Crude Extract and Solvent Fractions of Trichilia dregeana Sond (Meliaceae) Leaves in Mice

Evaluation of Wound Healing and Anti‐Inflammatory Activities of 80% Methanol Crude Extract and Solvent Fractions of Trichilia dregeana Sond (Meliaceae) Leaves in Mice

Nanophyto-gel against multi-drug resistant Pseudomonas aeruginosa burn wound infection

Evaluating the Effectiveness of Using Low-Level Laser Therapy for the Healing Process of Third-Degree Burns in Mice

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