Animal models of external traumatic wound infections

Dai, Tianhong; Kharkwal, Gitika B.; Tanaka, Masamitsu; Huang, Ying‐Ying; Arce, Vida J. Bil de; Hamblin, Michael R.

doi:10.4161/viru.2.4.16840

Cited by 136 publications

(118 citation statements)

References 153 publications

(167 reference statements)

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“…Mouse skin was then scraped with no. 15 scalpel blades until a reddened area appeared (just short of drawing blood) (7,23). This procedure resulted in first-degree skin abrasions with most of the epidermis removed (Fig.…”

Section: Methodsmentioning

confidence: 99%

“…Despite some differences, the immune systems of mice and humans are similar and they can often be challenged with the same or similar pathogens (3,7). In addition, mouse models have the advantage of using animals that are inexpensive to purchase and maintain, thereby allowing the performance of studies with enough samples to yield statistically significant conclusions.…”

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confidence: 99%

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UVC Light Prophylaxis for Cutaneous Wound Infections in Mice

Dai

Garcı́a

Murray

et al. 2012

Antimicrob Agents Chemother

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ABSTRACTUVC light has long been known to be highly germicidal but has not been much developed as a therapy for infections. This study investigated the potential of UVC light for the prophylaxis of infections developing in highly contaminated superficial cutaneous wounds.In vitrostudies demonstrated that the pathogenic bacteriaPseudomonas aeruginosaandStaphylococcus aureuswere inactivated at UVC light exposures much lower than those needed for a similar effect on mammalian keratinocytes. Mouse models of partial-thickness skin abrasions infected with bioluminescentP. aeruginosaandS. aureuswere developed. Approximately 107bacterial cells were inoculated onto wounds measuring 1.2 by1.2 cm on the dorsal surfaces of mice. UVC light was delivered at 30 min after bacterial inoculation. It was found that for both bacterial infections, UVC light at a single radiant exposure of 2.59 J/cm2reduced the bacterial burden in the infected mouse wounds by approximately 10-fold in comparison to those in untreated mouse wounds (P< 0.00001). Furthermore, UVC light increased the survival rate of mice infected withP. aeruginosaby 58.3% (P= 0.0023) and increased the wound healing rate in mice infected withS. aureusby 31.2% (P< 0.00001). DNA lesions were observed in the UVC light-treated mouse wounds; however, the lesions were extensively repaired by 48 h after UVC light exposure. These results suggested that UVC light may be used for the prophylaxis of cutaneous wound infections.

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Section: Methodsmentioning

confidence: 99%

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confidence: 99%

UVC Light Prophylaxis for Cutaneous Wound Infections in Mice

Dai

Garcı́a

Murray

et al. 2012

Antimicrob Agents Chemother

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“…147 PDT's very nature makes it ideal for the treatment of skin, wound and burn infections, all of which are easily accessible for light therapies. [148][149][150] XF73, a cationic porphyrin PS, is able to reduce MRSA growth by >3 log 10 in a porcine skin infection model. 151 PDT with polycationic PS conjugates and 665 nm light in murine excisional wounds led to a reduction in infectious organisms, permitting mouse survival to reach 90%, and reduce substantially pathogen viability.…”

Section: Acknowledgmentsmentioning

confidence: 99%

All you need is light

et al. 2011

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“…Increasing the degree of de-acetylation prolongs its degradation time while enhancing cell adhesion (Mao et al, 2004;Di Martino et al, 2005 In vivo differentiation to NP-like cells. Bertolo et al, 2012;Cheng et al, 2010;Cheng et al, 2013;Dai et al, 2011;Iwasaki et al, 2004;Li et al, 2005b;Mao et al, 2004;Di Martino et al, 2005;Patel et al, 2010;Pusateri et al, 2003;Richardson et al, 2008;Roughley et al, 2006;Shao and Hunter, 2007;Sun et al, 2014;Zhang et al, 2014 Alginate Brown seaweed Delayed drug delivery systems Cells cultured in 3D constructs, tested in vivo in murine model. Bron et al, 2011;Chou et al, 2009;Chou and Nicoll, 2009;Guo et al, 1989;Larsen and Haug, 1971;Leone et al, 2008;…”

Section: Natural Hydrogels Chitosanmentioning

confidence: 99%

“…Medical applications include wound haemostasis and healing, based on antimicrobial properties as well as drug delivery capabilities (Dai et al, 2011;Patel et al, 2010;Pusateri et al, 2003). Both hydrogels and solid scaffolds can be formed from chitosan (Di Martino et al, 2005).…”

Section: Natural Hydrogels Chitosanmentioning

confidence: 99%

Biomaterials for intervertebral disc regeneration: past performance and possible future strategies

Schutgens¹,

Tryfonidou²,

Smit³

et al. 2015

eCM

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Intervertebral disc (IVD) degeneration is associated with most cases of cervical and lumbar spine pathologies, amongst which chronic low back pain has become the number one cause of loss of quality-adjusted life years. In search of alternatives to the current less than optimal and usually highly invasive treatments, regenerative strategies are being devised, none of which has reached clinical practice as yet. Strategies include the use of stem cells, gene therapy, growth factors and biomaterial carriers. Biomaterial carriers are an important component in musculoskeletal regenerative medicine techniques. Several biomaterials, both from natural and synthetic origin, have been used for regeneration of the IVD in vitro and in vivo. Aspects such as ease of use, mechanical properties, regenerative capacity, and their applicability as carriers for regenerative and anti-degenerative factors determine their suitability for IVD regeneration. The current review provides an overview of the biomaterials used with respect to these properties, including their drawbacks. In addition, as biomaterial application until now appears to have been based on a mix of mere availability and intuition, a more rational design is proposed for future use of biomaterials for IVD regeneration. Ideally, high-throughput screening is used to identify optimally effective materials, or alternatively medium content comparative studies should be carried out to determine an appropriate reference material for future studies on novel materials.

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Animal models of external traumatic wound infections

Abstract: As antibiotic resistance continues to increase,more new antimicrobial approaches are urgently needed.These should be tested using standard protocols for infections in external traumatic wounds in animal models.

Cited by 136 publications

References 153 publications

UVC Light Prophylaxis for Cutaneous Wound Infections in Mice

UVC Light Prophylaxis for Cutaneous Wound Infections in Mice

All you need is light

Biomaterials for intervertebral disc regeneration: past performance and possible future strategies

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