Animal models of fulminant hepatic failure: A critical evaluation

Newsome, Philip N.; Plevris, John; Nelson, Leonard J.; Hayes, Peter

doi:10.1002/lt.500060110

Cited by 89 publications

(93 citation statements)

References 54 publications

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“…D-GalN'in i.p uygulanması hepatositlerde intrasellüler uridin trifosfat havuzunu azaltarak RNA ve protein sentezini engeller (23). Sıçanlarda i.p.…”

Section: Discussionunclassified

“…uygulandı-ğında serum transaminaz düzeylerinin artışına, hepatik nekroza ve komaya yol açar (24). D-GalN'in neden olduğu karaciğer lezyonlarının morfolojik ve patofizyolojik özel-liklerinin insan viral hepatit lezyonlarına birçok açıdan benzediği bildirilmiştir (23). İleri yaş bir pro-inflamatuar durumdur, inflamasyonun yükselmesi ile karakterizedir ve ileri yaşa bağlı kronik koşullarda birçok inflamatuar ve hemostaz belirteçlerinin artışı gözlenir.…”

Section: Discussionunclassified

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Evaluation of Tissue Factor Activities and Sialic Acid Levels in D-Galactose Induced Aging Model

Stundag¹,

Oktay²,

Emekli-Alturfan³

et al. 2014

MUSBED

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D-galaktoz ile oluşturulmuş yaşlanma modelinde doku faktörü aktivitesinin ve sialik asit miktarının değerlendirilmesi Amaç: Bu çalışmada D-galaktoz ile yaşlandırılmış, doğal yaşlı ve kontrol grubu sıçanların karaciğer ve renal dokularının doku faktörü (TF) aktivitelerinin ve inflamatuvar hastalıklar için değerli bir indikatör olan sialik asit (SA) miktarlarının ölçülmesi amaçlandı. Yöntemler: Çalışmada yirmi haftalık 28 adet Sprague-Dawley erkek rat kullanıldı. Gruplar; 1) genç kontrol grubu (n= 8), 2) doğal yaşlı erkek grup (n= 10), 3) 6 hafta süreyle 60 mg/kg/gün i.p. D-galaktoz enjekte edilen D-galaktoz ile yaşlandırılmış grup (n= 10) şeklinde oluşturuldu. İşlemler sonunda, ratlar bir gece aç bırakılarak sakrifiye edildi ve homojenizasyon için böbrek ve karaciğer dokuları toplandı. TF aktivitesi Quick metoduna, SA miktarı ise Warren'ın metoduna göre ölçüldü. Bulgular: D-galaktoz grubu böbrek dokusunda SA miktarı kontrol ve doğal yaşlı gruplara oranla anlamlı derecede azalmış, TF aktivitesi ise anlamlı derecede artmıştır. Karaciğer dokusu SA miktarında gruplar arasında anlamlı bir değişiklik gözlenmemiştir. Karaciğer TF aktivitesi ise D-galaktoz grubunda kontrol grubuna göre artmıştır. Sonuç: D-galaktoz ile oluşturulan yaşlanma modelinde böbrek ve karaciğer dokularında meydana gelen TF aktivitesi artışı ve böbrek SA seviyelerindeki azalma D-galaktozun yaşlanma ile ilgili tromboz/hemostaz çalışmalarında kullananılabileceğini göstermektedir. Anahtar sözcükler: D-galaktoz, yaşlanma, doku faktörü, sialik asit ABS TRACT Evaluation of tissue factor activities and sialic acid levels in D-galactose induced aging modelObjective: The aims of this study are to evaluate the changes in tissue factor (TF) activities and sialic acid (SA) concentrations of D-galactose induced rat hepatic and renal tissues and compare with the naturally aged and the control groups. Methods: In this study Sprague-Dawley male rats (n=28, 20 weeks old) were used. The experiment and the control groups were organized as follows: 1) young male control group (n= 8, 20 weeks old). 2) naturally aged male group (n= 10) 3) D-Galactose induced ageing group (D-galactose injected (60 mg/kg/day) for 6 weeks, n= 8, 20-week-old). At the end of the treatment, the rats were sacrificed following an overnight fast, kidney and liver tissues were immediately collected for homogenization. TF activity and SA levels were measured by the methods of Quick and Warren respectively. Results: SA levels of kidney decreased whereas TFa (TF activity) increased significantly in the D-galactose induced aging group when compared with the control and the naturally aged groups. SA levels of liver did not change significantly between the groups. TFa of liver increased significantly compared with the control group. Conclusion: Increased TFa (TF activity) of kidney and liver and decreased SA levels of kidney in D-galactose induced aging model show that this model can be used in studies related with aging, thrombosis and haemostasis.

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“…D-GalN'in i.p uygulanması hepatositlerde intrasellüler uridin trifosfat havuzunu azaltarak RNA ve protein sentezini engeller (23). Sıçanlarda i.p.…”

Section: Discussionunclassified

Evaluation of Tissue Factor Activities and Sialic Acid Levels in D-Galactose Induced Aging Model

Stundag¹,

Oktay²,

Emekli-Alturfan³

et al. 2014

MUSBED

View full text Add to dashboard Cite

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“…On the other hand, in living donor liver transplantation using extremely small-sized grafts, the transplanted graft could be supported by the remnant native liver [4,16]. As possible applications of the current APOLT model, several experiments could be designed using rats with chemically or surgically induced fulminant hepatitis [17], metabolically diseased rats [18], marginal donor grafts like fatty grafts [19]. Now we are investigating the use of warminjured grafts from non-heart-beating donors using the current APOLT model.…”

Section: Discussionmentioning

confidence: 99%

A Novel Auxiliary Partial Orthotopic Liver Transplantation Model in Rats

et al. 2000

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Background: Although auxiliary partial orthotopic liver transplantation (APOLT) has become a well-accepted procedure recently, a practical experiment model in APOLT using small animals has yet to be developed. Methods: Male Lewis rats were used for both donors and recipients. An auxiliary partial graft was obtained by ex vivo resection of the donor right and caudate lobes, and was transplanted orthotopically into the recipient after resection of the recipient medial and left hepatic lobes. Portal vein and hepatic duct reconstructions were by the cuff technique, and supra- and intrahepatic vena cava were sutured continuously. Operative outcomes, serum chemistry, liver tissue blood flow, angiographic and histopathological findings were then examined. Conventional orthotopic liver transplantation (OLT) procedures were also undertaken as a control. Results: One-day, 1-week and 1-month survival rate of APOLT group was 100, 85 and 85%, respectively. AST in the APOLT group on the 1st postoperative day was significantly higher than in the OLT group. No significant differences were recognized in serum albumin and total bilirubin levels between the two groups. Although the portogram of an APOLT rat showed slight narrowing at the cuff anastomosis site, both the graft and the native liver were opacified similarly. The liver tissue blood flow on the 5th postoperative day in the native liver and the graft returned to as high as 95 and 74% of the values on laparotomy, respectively. Histological examinations of the auxiliary graft 1 month after transplantation showed mild ductular proliferation and mononuclear cell infiltration around the portal triads. Conclusion: This novel APOLT model in rats allows practical and reproducible results, and may be of value in the basic study of APOLT procedures.

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“…In the worldwide literature, the model for inducing acute liver failure via toxins in rats has been mostly used to test support therapies consist of d-galactosamine intraperitoneal administration, because it is potentially reversible, presenting non-significant extra-hepatic toxicity and has the best-defined hepatotoxic dosage 10 .…”

Section: Introductionmentioning

confidence: 99%

Evaluating the best time to intervene acute liver failure in rat models induced by d-galactosamine

et al. 2016

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PURPOSE:To describe an animal model for acute liver failure by intraperitoneal d-galactosamine injections in rats and to define when is the best time to intervene through King's College and Clichy´s criteria evaluation. METHODS:Sixty-one Wistar female rats were distributed into three groups: group 1 (11 rats received 1.4 g/kg of d-galactosamine intraperitoneally and were observed until they died); group 2 (44 rats received a dose of 1.4 g/kg of d-galactosamine and blood and histological samples were collected for analysis at 12 , 24, 48 , 72 and 120 hours after the injection); and the control group as well (6 rats). RESULTS:Twelve hours after applying d-galactosamine, AST/ALT, bilirubin, factor V, PT and INR were already altered. The peak was reached at 48 hours. INR > 6.5 was found 12 hours after the injection and factor V < 30% after 24 hours. All the laboratory variables presented statistical differences, except urea (p = 0.758). There were statistical differences among all the histological variables analyzed. CONCLUSION:King's College and Clichy´s criteria were fulfilled 12 hours after the d-galactosamine injection and this time may represent the best time to intervene in this acute liver failure animal model.

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Animal models of fulminant hepatic failure: A critical evaluation

Cited by 89 publications

References 54 publications

Evaluation of Tissue Factor Activities and Sialic Acid Levels in D-Galactose Induced Aging Model

Evaluation of Tissue Factor Activities and Sialic Acid Levels in D-Galactose Induced Aging Model

A Novel Auxiliary Partial Orthotopic Liver Transplantation Model in Rats

Evaluating the best time to intervene acute liver failure in rat models induced by d-galactosamine

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