Evaluating the best time to intervene acute liver failure in rat models induced by d-galactosamine

Éboli, Lígia Patrícia de Carvalho Batista; Netto, Alcides Augusto Salzedas; Azevedo, Ramiro Antero de; Lanzoni, Valéria Pereira; Paula, Tatiana Sugayama de; Goldenberg, Alberto; Gonzalez, Adriano Miziara

doi:10.1590/s0102-865020160120000002

Cited by 4 publications

(2 citation statements)

References 16 publications

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“…Before ultrasound radiation, 300 μL of the microbubble suspension was injected slowly via the tail vein. The other acoustic parameters were as follows: 1 MHz, 20% duty cycle, and exposure time of 10 min, which was determined based on previous literature examples [15, 16]. The rats were sacrificed at 48 h after UTMD, and the collected livers were stored in liquid nitrogen.…”

Section: Methodsmentioning

confidence: 99%

A combination of ultrasound-targeted microbubble destruction with transplantation of bone marrow mesenchymal stem cells promotes recovery of acute liver injury

Sun

Gao

et al. 2018

Stem Cell Res Ther

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BackgroundBone marrow mesenchymal stem cells (BMSCs) can provide an additional source of therapeutic stem cells for regeneration of liver cells during acute liver injury (ALI). However, the insufficient hepatic homing by the transplanted BMSCs limits their applications. Ultrasound-targeted microbubble destruction (UTMD) has been reported to promote the homing of transplanted stem cells into the ischemic myocardium. In this study, we investigated whether UTMD promotes the hepatic homing of BMSCs in ALI rats and evaluated the therapeutic effect.MethodsBMSCs were isolated from the femurs and tibias of Sprague-Dawley (SD) rats. The isolated BMSCs were stably transfected with a lentivirus expressing enhanced green fluorescent protein (EGFP) that can be visualized and quantified in vivo after transplantation. Both tumor necrosis factor α (TNF-α) and stromal cell-derived factor 1 (SDF-1) were used to verify the appropriate ultrasound parameters. The ALI rats were divided into four groups: control, BMSCs, UTMD, and UTMD + BMSCs. The protein and mRNA expression levels of SDF-1, intercellular cell adhesion molecule (ICAM-1), vascular cell adhesion molecule 1 (VCAM-1), hepatocyte growth factor (HGF), and monocyte chemotactic protein 1 (MCP-1) in the exposed livers were analyzed at 48 h after treatment. ALI recovery was determined by serum biochemical parameters and histology.ResultsThe isolated rat BMSCs demonstrated a good proliferation potential that was both osteogenic and adipogenic in differentiation and expressed cluster of differentiation (CD) 29 and CD90, but not CD45 or CD11b/c. After BMSC and/or UTMD treatment, the number of GFP-labeled BMSCs in the UTMD + BMSCs group was significantly higher than that of the BMSCs group (9.8 ± 2.3 vs. 5.2 ± 1.1/per high-power field). Furthermore, the expression of GFP mRNA was performed for evaluation of the homing rate of BMSCs in injury sites as well. In addition, the expression levels of SDF-1, ICAM-1, VCAM-1, HGF, and MCP-1 were higher (p < 0.01) in UTMD+BMSCs group. The serum levels of biomarkers were significantly lower in the UTMD + BMSCs group, and the apoptotic rate of hepatocytes in the UTMD + BMSCs group was markedly lower than that of the BMSCs group (all p < 0.05). The hepatic pathology was significantly alleviated in the UTMD + BMSCs group.ConclusionsUTMD treatment efficiently induced a favorable microenvironment for cell engraftment, resulting in improvement of hepatic homing of BMSCs, which was probably mediated through upregulation of the expression of adhesion molecules and cytokines. UTMD treatment appeared to be an effective and noninvasive approach to achieve better efficacy of BMSC-based therapy for repairing a severely injured liver.Electronic supplementary materialThe online version of this article (10.1186/s13287-018-1098-4) contains supplementary material, which is available to authorized users.

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Section: Methodsmentioning

confidence: 99%

A combination of ultrasound-targeted microbubble destruction with transplantation of bone marrow mesenchymal stem cells promotes recovery of acute liver injury

Sun

Gao

et al. 2018

Stem Cell Res Ther

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“…D-GaIN is an amino acid derivative and a highly hepatospecific toxin. The action of D-GaIN is to reduce the number of unbound uridine molecules, which prevents the synthesis of macromolecules, including RNA and protein, and causes massive necrosis of hepatocytes [ 7 ]. Just one dose of D-GaIN administered intraperitoneally can cause focal hepatocyte necrosis and a range of effects very similar to those observed in human viral hepatitis [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Comparison of the Efficacy of Two Routes of Administration of Human Amniotic Epithelial Cells in Cell Therapy of Acute Hepatic Insufficiency

Wieczorek,

Czekaj,

Król

et al. 2024

Pharmaceuticals

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The route of administration of implanted cells may affect the outcome of cell therapy by directing cell migration to the damaged site. However, the question of the relationship between the route of administration, the efficacy of colonisation of a given organ, and the efficacy of cell therapy has not been resolved. The aim of the study was to localise transplanted intravenously and intraperitoneally human amniotic epithelial cells (hAECs) in the tissues of mice, both healthy and injured, in an animal experimental model of acute liver failure (ALF). Mice intoxicated with D-Galactosamine (D-GalN) at a dose of 150 mg/100 g body weight received D-GalN alone or with a single dose of hAECs administered by different routes. Subsequently, at 6, 24, and 72 h after D-GaIN administration and at 3, 21, and 69 h after hAEC administration, lungs, spleen, liver, and blood were collected from recipient mice. The degree of liver damage and regeneration was assessed based on biochemical blood parameters, histopathological evaluation (H&E staining), and immunodetection of proliferating (Ki67+) and apoptotic (Casp+) cells. The biodistribution of the administered cells was based on immunohistochemistry and the identification of human DNA. It has been shown that after intravenous administration, in both healthy and intoxicated mice, most of the transplanted hAECs were found in the lungs, while after intraperitoneal administration, they were found in the liver. We concluded that a large number of hAECs implanted in the lungs following intravenous administration can exert a therapeutic effect on the damaged liver, while the regenerative effect of intraperitoneally injected hAECs on the liver was very limited due to the relatively lower efficiency of cell engraftment.

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A bioluminescent probe for in vivo imaging of pyroglutamate aminopeptidase in a mouse model of inflammation

Chen

Qin

et al. 2021

Bioorganic & Medicinal Chemistry Letters

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Evaluating the best time to intervene acute liver failure in rat models induced by d-galactosamine

Cited by 4 publications

References 16 publications

A combination of ultrasound-targeted microbubble destruction with transplantation of bone marrow mesenchymal stem cells promotes recovery of acute liver injury

A combination of ultrasound-targeted microbubble destruction with transplantation of bone marrow mesenchymal stem cells promotes recovery of acute liver injury

Comparison of the Efficacy of Two Routes of Administration of Human Amniotic Epithelial Cells in Cell Therapy of Acute Hepatic Insufficiency

A bioluminescent probe for in vivo imaging of pyroglutamate aminopeptidase in a mouse model of inflammation

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