Animal Models of Myocardial and Vascular Injury

Abarbanell, Aaron M.; Herrmann, Jeremy L.; Weil, Brent R.; Wang, Yue; Tan, Jiangning; Moberly, Steven P.; Fiege, Jeremy W.; Meldrum, Daniel R.

doi:10.1016/j.jss.2009.06.021

Cited by 55 publications

(46 citation statements)

References 102 publications

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“…1,2 To this end, it is necessary to establish the infarct area. Histological techniques are available, but their application is limited since multiple, and longitudinal assessments of the myocardial injury are not possible.…”

Section: Introductionmentioning

confidence: 99%

Rubidium-82 PET imaging is feasible in a rat myocardial infarction model

Ghotbi

Clemmensen

Kyhl

et al. 2019

Journal of Nuclear Cardiology

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Background. Small-animal myocardial infarct models are frequently used in the assessment of new cardioprotective strategies. A validated quantification of perfusion using a noncyclotron-dependent PET tracer would be of importance in monitoring response to therapy. We tested whether myocardial PET perfusion imaging is feasible with Rubidium-82 ( 82 Rb) in a small-animal scanner using a rat myocardial infarct model.Methods. 18 Sprague-Dawley rats underwent permanent coronary artery ligation (infarct group), and 11 rats underwent ischemia-reperfusion (reperfusion group) procedure.82 Rb-PET and magnetic resonance imaging (MRI) were conducted before and after the intervention. Perfusion was compared to both left ventricle ejection fraction (LVEF) and infarct size assessed by MRI.Results. Follow-up global 82 Rb-uptake correlated significantly with infarct size (infarct group: r 5 20.81, P < 0.001 and reperfusion group: r 5 20.61, P 5 0.04). Only 82 Rb-uptake in the infarct group correlated with LVEF. At follow-up, a higher segmental 82 Rb-uptake in the infarct group was associated with better wall motion (b 5 0.034, CI [0.028;0.039], P < 0.001, R 2 5 0.30), and inversely associated with scar transmurality (b 5 22.4 [22.6; 22.2], P < 0.001, R 2 5 0.59). The associations were similar for the reperfusion group. Conclusion.82 Rb-PET is feasible in small animal scanners despite the long positron range and enables fast and time-efficient myocardial perfusion imaging in rat models. (J Nucl Cardiol 2017) Key Words: Small-animal heart AE rat myocardium AE perfusion imaging AE infarction AE rubidium-82 PET AE magnetic resonance Electronic supplementary material The online version of this article

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Section: Introductionmentioning

confidence: 99%

Rubidium-82 PET imaging is feasible in a rat myocardial infarction model

Ghotbi

Clemmensen

Kyhl

et al. 2019

Journal of Nuclear Cardiology

View full text Add to dashboard Cite

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“…There are multiple reasons for the lack of effective extrapolation of experimental data to clinical settings. The most often addressed are the redundant signaling pathways and compensative mechanisms existing in pathogenic process of myocardial infarction induced by any single cause [5][6][7]. However, the most troublesome encounter of current experimental studies is the physiological and pathological compatibility between rodent models and humans [8].…”

Section: Introductionmentioning

confidence: 99%

Electrocardiographic Characterization of Rhesus Monkey Model of Ischemic Myocardial Infarction Induced by Left Anterior Descending Artery Ligation

et al. 2011

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Myocardial infarction is a leading cause for morbidity and mortality in the modern society. Rhesus monkeys are excellent animal models for experimental and translational studies of cardiovascular diseases in humans. However, some detailed characterizations of cardiovascular disease, such as myocardial infarction, in Rhesus monkeys have not been available. The present study was undertaken to examine the progressive electrocardiographic changes in Rhesus monkeys after left anterior descending (LAD) artery ligation. Male Rhesus monkeys, aged 2-3 years and weighed 4.5-6.0 kg, were subjected to LAD ligation along with sham-operated controls. At 1 week, 1 month, and 6 months after the LAD ligation, ECG recording was performed to detect the progressive changes in ECG. In addition, cardiac magnetic resonance imaging (MRI) and echocardiography were applied to detect the myocardial infarction induced by LAD ligation, and histopathological examination was performed at the end of the experiment to measure the morphological changes. The results showed that QRS and ST-T changed significantly within 1 month after LAD ligation, but recovered to normal at the end of the experiment. The most significant change was a progressive QTc prolongation, which occurred corresponding to the development of myocardial infarction. Both cardiac MRI and echocardiography detected the myocardial infarction that was confirmed by the histopathological examination. This detailed characterization of ECG changes along with the development of myocardial infarction induced by LAD ligation thus demonstrated that the Rhesus monkey model of ischemic myocardial infarction would be an excellent surrogate for human myocardial infarction. This model would also provide an excellent tool for drug discovery and development for cardiac disease.

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“…We believe the selective CVBG should be considered in cases of coronary artery disease not amenable to traditional revascularization strategies [42][43][44][45]. Indications of selective CVBG include the patients with tenuous right coronary artery or diffuse lesions.…”

Section: Resultsmentioning

confidence: 99%