1991
DOI: 10.1073/pnas.88.14.6117
|View full text |Cite
|
Sign up to set email alerts
|

Anionic subsites of the catalytic center of acetylcholinesterase from Torpedo and from cobra venom.

Abstract: A peptide of acetylcholinesterase (AcChoEase; acetylcholine acetylhydrolase, EC 3.1.1.7) from the venom of the cobra Naja naja oxiana labeled by the affinity reagent N,N-dimethyl-2-phenylaziridinium (DPA) has been identified. The sequence is Gly-Ala-Glu-Met-Trp-Asn-Pro-Asn. In AcChoEase from Torpedo californica, a homologous peptide was labeled and isolated. Its sequence is Ser-Gly-Ser-Glu-Met-Trp-Asn-Pro-Asn, representing positions 79 through 87. In both cases labeling can be prevented by 0.1 mM edrophonium, … Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
2
1
1
1

Citation Types

3
19
1

Year Published

1991
1991
2012
2012

Publication Types

Select...
9
1

Relationship

0
10

Authors

Journals

citations
Cited by 45 publications
(23 citation statements)
references
References 46 publications
3
19
1
Order By: Relevance
“…At substrate concentrations greater than 1 mM krait venom AChE hydrolytic property was reduced ( Figure 1). Similar substrate inhibition was observed with other snake venom acetylcholinesterase (10,24,25).…”
Section: Discussionsupporting
confidence: 66%
“…At substrate concentrations greater than 1 mM krait venom AChE hydrolytic property was reduced ( Figure 1). Similar substrate inhibition was observed with other snake venom acetylcholinesterase (10,24,25).…”
Section: Discussionsupporting
confidence: 66%
“…In particular, AChE may be purified in significant quantities from the venom of Bungarus fasciatus. In this report, we show that this enzyme presents a strong sequence homology with AChEs from various invertebrate and vertebrate species, in agreement with previous studies on AChE from the venom of Naja naja oxiana [4,5]. It differs, however, from AChEs from other vertebrate tissues by the fact that it consists entirely of soluble, non-amphiphilic monomers.…”
Section: Ache Assaysupporting
confidence: 76%
“…This is in apparent contradiction to a previous report from Kreienkamp et al (1991) who claimed that cobra venom acetylcholinesterase (Nuju nuju oxiunu) is devoid of a peripheral site.…”
Section: Discussioncontrasting
confidence: 54%