Anisotropic nanocrystal arrays organized on protein lattices formed by recombinant clathrin fragments

Abstract

Recombinant clathrin protein fragments form assemblies that template gold nanocrystals in an array across the latticed surface. The nanocrystals exhibit unusual anisotropic morphologies with long range ordering, both of which are dependent upon the presence of a hexahistidine tag on the clathrin heavy chain fragments.

Protein-aided formation of triangular silver nanoprisms with enhanced SERS performance

“…[62][63][64][65] In particular, the terminal polyhistidine and cysteine tag is often deliberately engineered into the protein or peptide for the controlled assembly or patterning of NPs. [66][67][68][69][70] To better understand the tolerance of the described synthetic strategy to modifications of CTPR3, we performed Ag NT synthesis using either CTPR3 with a N-terminal polyhistidine tag or CTPR3 with an engineered C-terminal cysteine. Although the UV-Vis spectra of colloidal solution containing Ag seeds are identical in all cases (Fig.…”

Repeat protein mediated synthesis of gold nanoparticles: effect of protein shape on the morphological and optical properties

“…1 An area of growing interest is the harnessing of biomolecules for the synthesis and assembly of nanoparticles under mild ambient conditions for biosensing and catalysis. 2,3 Bioenabled strategies draw inspiration from nature where materials with unique structures, compositions, and functions are achieved in the process of biomineralization through the interaction of inorganic material with bioscaffold such as peptides, 4-10 proteins [11][12][13][14][15][16][17] and nucleic acids. [18][19][20][21][22][23][24] In particular, extensive research has focused on the biomimetic synthesis of Au NPs as the fundamental building blocks for the construction of catalytic, optoelectronic and biosensing devices.…”

“…12,25,26 To date, a broad spectrum of natural and recombinant proteins were exploited as biotemplates for the synthesis of Au NPs with tailored structures. [11][12][13][14]16 In a representative example, anisotropic Au NPs arrays have been integrated on the self-assembled protein clathrin Hub-His6 through a two-step, seed-mediated growth process. In another study, a tetrapod shape was achieved through the coordination of in situ prepared Au nanoclusters with the trimeric gp5-His6 protein.…”

Tunable Conformation-Dependent Engineered Protein·Gold Nanoparticle Nanocomposites

“…31 Both C and Q contained an N-terminal hexa-histidine tag (6-His) that could be eliminated to produce Cx and Qx, respectively (Table 1). 14 Successful cleavage of the 6-His tag from C and Q by Factor Xa was confirmed by sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE, Figure S1), with a molecular weight of the 6-His proteins corresponding to 6.31 kDa for C and Q versus 4.45 kDa for the corresponding cleaved proteins Cx and Qx (Table 1).…”