Anisotropic Scattering by Single Starch Granules

Borch, Jens; Sarko, A.; Marchessault, R. H.

doi:10.1002/star.19690211102

Cited by 24 publications

(11 citation statements)

References 5 publications

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“…This may be due to the fact that FALS and absorbance of light by cells and cellular components has been shown to be affected by variables (i. e. particles asymmetry, refractive index, , and reflective properties) that are not directly related to size [ll]. In addition, it has been shown that some large particles scatter less light than theoretically expected [12]. Thus, the ' appearance of granules in a step or pulse-like pattern rather than a tight linear function my be due to differences in morphological or internal structure differences within each size class.…”

Section: Analysis Of Starch Granulesmentioning

confidence: 99%

Flow Cytometric Determination of Light Scattering and Absorbance Properties of Starch Granules

Jones

Srienc

Roessler³

et al. 1992

Starch Stärke

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Treatment of samples of digested endosperm with SDS resulted in a homogenous suspension of singlc starch granules that was suitable for analysis by flow cytometry without altering their refractive properties. Flow cytometry can be used for more rapid and accurate enumeration of starch granules than can be achieved with a hemocytometer as with the latter the number of starch granules seems to be underestimated. Moreover. flow cytometric analysis based on forward angle light scatter (FALS) and absorbance properties revealed that there are at least three unique subclasses of starch granules that appear during kernel development. The first subclass contained mainly small granules (2-5 km) whereas the second subclass generally consisted of large granules (> 5 krn). However, within a given size. there were two distinct subpopulations with significantly dillerent absorbance properties. The physiological or physicochemical significants of these subclasses warrant further investigation. FlieO-zytometrische Bestimmung der Lichtstreuungs-und -absorptionseigenschaften von Starkekornern. Die Behandlung von abgebauten Endospermproben mit SDS (Natrium-dodecylsulfat) ergab eine homogene Suspension einzelner Starkekorner. die fur die Analyse mittels FlieR-Zytometrie geeigiiet war. ohne ihre Refraktionseigenschaften zu verandern. FlieB-Zytometrie kann fur schnellere und gcnauere Auszahlung von Storkekornern angewendet werden als sie mit einem Haniozytometer erreicht werden kann. zumal mit letzterem die Anzahl an Starkekorncrn untcrschatzt zu werden scheint. Daruber hinaus basiert die flie8-zytometrische Analyse auf der Vorwartswinkel-Lichtstreuung (FALS), und die Absorptionseigenschaften zeigten. daB cs mindcstens drei einheilliche Unterklassen von Starkekornern gibt, die wahrend der Kornentwicklung auftreten. Die erste Unterklasse enthielt hauptsachlich kleine Korner (2-5 pn). wogegen die zweite Unterklassc allgcmein aus gro13en Kornern hestand (> 6 pn). Allerdings lagen innerhalb einer gegebenen GroBe zwei verschiedene linterpopulationen mit deutlich unterschiedlichen Absorptionseigenschaften vor. Die physiologischen und physikalisch-chemischen Merkmale dieser Unterklassen bedurfen weitere Untersuchung.

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Section: Analysis Of Starch Granulesmentioning

confidence: 99%

Flow Cytometric Determination of Light Scattering and Absorbance Properties of Starch Granules

Jones

Srienc

Roessler³

et al. 1992

Starch Stärke

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“…3 in Ref. 1). The presence and clarity of these maxima is important, because as will be shown later, the relative scattered intensity distribution among them is much more sensitive to internal morphology of the granule than is the primary cloverleaf.…”

Section: H Scatteringmentioning

confidence: 87%

“…The quantitative intensity distribution of the pattern in Figure 8 is shown in Figure 9, to be compared with the calculated theoretical distribution for a perfectly spherical and homogeneously anisotropic granule, shown in a,)(SiUsin U ) -(atar) cos2 (812) cos2 p X (4 sin U -U cos U -3SiU)I2 @) where as is the polarizability of the surrounding matrix and other quantities have the same significance as in cq. (1) .' It is apparcnt from the above equation that only the third term in brackets depends on the anisotropy of the spherulite.…”

Section: Scatteringmentioning

confidence: 99%

Anisotropic scattering by single starch granules. II. Layered granule structure

Finkelstein

Sarko

1972

Biopolymers

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SynopsisThe study of anisotropy light scattering from tapioca and potato starches has continued with the recording of more detailed experimental single-granule H , scattering patterns and, for the first time, singlegranule V , patterns. Quantitative analysis of the relative intensity distribution in experimental H , patterns reveals the sensitivity of the higher order scattering maxima to the granule morphology, permitting an analysis of the latter in terms of a layered structure. For tapioca starch, this analysis indicates that if layering is present a t all, the layer thickness is comparable to the wavelength of the incident radiation, and most likely is considerably less than 0.5 p in thickness. On the other hand, the potato starch morphology is characterized by a relatively coarse layering with few layers and considerable difference in the anisotropy between successive layers. The models for the two starches in best agreement with experimental data are as follows: almost perfectly spherulitic anisotropic structure with very thin shell-like layers-if any-for tapioca, and alternating layers of varying anisotropy several microns in thickness and probably simultaneously present with an isotropic center, for potato starch. The V , pattern for tapioca starch is in agreement with this model, although its information content is lower owing to the experimental difficulty of recording higher order maxima. Suggestions for further morphological study of starches are presented.

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“…Quantitative density measurements on the latter films were performed with a *Vertically polarized incident beam, analyzer with polarization direction horizontal. Joyce-Loebl microdensitometer as previously described [4] . All computations were performed on an IBM 1620-11 computer.…”

Section: Methodsmentioning

confidence: 99%

Light scattering by cellulose. V. Anisotropy scattering by wood fibers

Müggli

Marton

Sarko

1971

J. polym. sci., C Polym. symp.

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Solid state light scattering patterns from single wood fibers were recorded using a laser as the light source. The scattering was recorded in the Hv mode; i.e., with the incident light beam vertically polarized, the scattered light was passed through a horizontal analyzer, thus permitting the recording of only the scattering arising from anisotropy fluctuations. The scattering pattern was confined to a narrow line consisting of regularly spaced maxima and minima. The overall scattering envelope could be accounted for by a hollow cylindrical model with helically wound dipoles, while the periodicity in the intensity is a function of fiber diameter and cell wall thickness. Comparison of relative intensities led to the conclusions that cellulose of the wood fiber behaves optically as a biaxial crystal in that the cellulose crystallites are biaxially oriented in an axial‐tangentoplanar fashion in the cell wall.

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Anisotropic Scattering by Single Starch Granules

Cited by 24 publications

References 5 publications

Flow Cytometric Determination of Light Scattering and Absorbance Properties of Starch Granules

Flow Cytometric Determination of Light Scattering and Absorbance Properties of Starch Granules

Anisotropic scattering by single starch granules. II. Layered granule structure

Light scattering by cellulose. V. Anisotropy scattering by wood fibers

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