antiferromagnetic materials with room-temperature magnetism and ambient stability are highly desirable for practical applications of antiferromagnetic spintronic nanodevices; however, they have been rarely explored. Here, a new family of antiferromagnetic transition-metal borate TMBO 3 (TM = V, Cr, Mn, Fe) nanosheets, 2D counterparts of calcite-type TMBO 3 materials, are reported based on first-principles calculations. 2D TMBO 3 crystals are antiferromagnetic semiconductors with band gaps ranging from 2.92 to 5.09 eV and high lattice thermodynamic stability up to 1500 K. Particularly, the estimated Neél temperature (T N ) of a 2D CrBO 3 crystal is 397 K, which can be further enhanced to 672 K by applying a biaxial strain. Strong chemical bonding between transition-metal cations and [BO 3 ] 3− anions endows 2D TMBO 3 crystals with ambient stability against oxidative degradation and molecular adsorption on the surface. This study provides a promising prototype of antiferromagnetic TMBO 3 nanosheets for practical applications of antiferromagnetic spintronic nanodevices, such as read-head devices and magnonic transport-based devices.