Annao Pingchong decoction alleviate the neurological impairment by attenuating neuroinflammation and apoptosis in intracerebral hemorrhage rats

Guo, Chunxian; Zhou, Xinrui; Wang, Xu; Wang, Haojie; Liu, Jian; Wang, Jinxi; Lin, Xiaoyuan; Lei, Shihui; Yang, Yi; Liu, Kai; Long, Hong‐Ping; Zhou, Desheng

doi:10.1016/j.jep.2023.116298

Cited by 5 publications

(8 citation statements)

References 45 publications

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“…A normal score is 0, and higher scores reflect more severe neurological deficits. 53 The behavioral tests were conducted in a double-blind manner.…”

Section: Methodsmentioning

confidence: 99%

Trifluoperazine regulates blood-brain barrier permeability via the MLCK/p-MLC pathway to promote ischemic stroke recovery

Zhang,

Chen,

et al. 2024

iScience

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“…A normal score is 0, and higher scores reflect more severe neurological deficits. 53 The behavioral tests were conducted in a double-blind manner.…”

Section: Methodsmentioning

confidence: 99%

Trifluoperazine regulates blood-brain barrier permeability via the MLCK/p-MLC pathway to promote ischemic stroke recovery

Zhang,

Chen,

et al. 2024

iScience

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“…Meanwhile, a network of disease-herb-active ingredient-target was obtained from symmap database (https://www.symmap.org/). Then, active ingredients were further screened based on 48 active plasma components of ANPCD found in a previous study, 11 and targets were further screened based on the filter conditions for Degree >1.…”

Section: Network Pharmacology Analysismentioning

confidence: 99%

“…The components of ANPCD were shown in Supplementary Table1, and its preparation was conducted as our previous study mentioned. 11 Edaravone and dexborneol concentrated solution for injection (ED), a clinical drug for alleviating oxidative stress damage in cerebral infarction, was obtained from Simcere Pharmaceutical Co. Ltd. (Nanjing, China).…”

Section: Drug Preparationmentioning

confidence: 99%

“…The clinical and in vivo experiments have demonstrated the efficacy of ANPCD in alleviating nerve function deficits in patients and reducing neuroinflammation and cerebral edema in ICH rats. [11][12][13][14][15][16] Modern pharmacological studies have identified 93 compounds in ANPCD, including baicalin, baicalein, wogonin, oroxylin A, geniposide, genipin, crocin, rhein, emodin, rhynchophylline, isorhynchophylline, and hirsutine. These compounds have exhibited neuroprotective effects through their antioxidant activity in various central nervous system diseases.…”

Section: Introductionmentioning

confidence: 99%

“…These compounds have exhibited neuroprotective effects through their antioxidant activity in various central nervous system diseases. 11,[17][18][19][20][21][22][23][24] These studies suggest that ANPCD may exert its therapeutic effects on ICH through antioxidative mechanisms, but the specific mechanisms have not been elucidated. So this study will investigate the effects of ANPCD on OS after ICH to elucidate its neuroprotection.…”

Section: Introductionmentioning

confidence: 99%

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Integrated Network Pharmacology and in vivo Experimental Validation Approach to Explore the Potential Antioxidant Effects of Annao Pingchong Decoction in Intracerebral Hemorrhage Rats

Zhou,

Wang,

et al. 2024

DDDT

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Background: Annao Pingchong decoction (ANPCD) is a traditional Chinese decoction which has definite effects on treating intracerebral hemorrhage (ICH) validated through clinical and experimental studies. However, the impact of ANPCD on oxidative stress (OS) after ICH remains unclear and is worth further investigating. Aim: To investigate whether the therapeutic effects of ANPCD on ICH are related to alleviating OS damage and seek potential targets for its antioxidant effects. Materials and Methods: The therapeutic candidate genes of ANPCD on ICH were identified through a comparison of the target genes of ANPCD, target genes of ICH and differentially expressed genes (DEGs). Protein-protein interaction (PPI) network analysis and functional enrichment analysis were combined with targets-related literature to select suitable antioxidant targets. The affinity between ANPCD and the selected target was verified using macromolecular docking. Subsequently, the effects of ANPCD on OS and the selected target were further investigated through in vivo experiments. Results: Forty-eight candidate genes were screened, in which silent information regulator sirtuin 1 (SIRT1) is one of the core genes that has antioxidant effects and ICH significantly affected its expression. The good affinity between 6 compounds of ANPCD and SIRT1 was also demonstrated by macromolecular docking. The results of in vivo experiments demonstrated that ANPCD significantly decreased modified neurological severity scoring (mNSS) scores and serum MDA and 8-OHdG content in ICH rats, while significantly increasing serum SOD and CAT activity, complicated with the up-regulation of ANPCD on SIRT1, FOXO1, PGC-1α and Nrf2. Furthermore, ANPCD significantly decreased the apoptosis rate and the expression of apoptosis-related proteins (P53, cytochrome c and caspase-3). Conclusion: ANPCD alleviates OS damage and apoptosis after ICH in rats. As a potential therapeutic target, SIRT1 can be effectively regulated by ANPCD, as are its downstream proteins.

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Synergistic promotion of angiogenesis after intracerebral hemorrhage by ginsenoside Rh2 and chrysophanol in rats

Li,

Zhou,

Pan

et al. 2024

Bioorganic Chemistry

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Annao Pingchong decoction alleviate the neurological impairment by attenuating neuroinflammation and apoptosis in intracerebral hemorrhage rats

Cited by 5 publications

References 45 publications

Trifluoperazine regulates blood-brain barrier permeability via the MLCK/p-MLC pathway to promote ischemic stroke recovery

Trifluoperazine regulates blood-brain barrier permeability via the MLCK/p-MLC pathway to promote ischemic stroke recovery

Integrated Network Pharmacology and in vivo Experimental Validation Approach to Explore the Potential Antioxidant Effects of Annao Pingchong Decoction in Intracerebral Hemorrhage Rats

Synergistic promotion of angiogenesis after intracerebral hemorrhage by ginsenoside Rh2 and chrysophanol in rats

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