Annealing multicomponent supramolecular gels

Fuentes-Caparrós, Ana M.; Gómez-Franco, Francisco de Paula; Dietrich, Benjamin; Wilson, Claire; Brasnett, Christopher; Seddon, Annela M.; Adams, Dave J.

doi:10.1039/c8nr09423c

Cited by 31 publications

(28 citation statements)

References 44 publications

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“… 22 , 23 Furthermore, coassembly of two different building blocks into one ordered structure demonstrated improvements in the mechanical properties, stability, and biofunctionality as compared to hydrogels assembled from each of the individual components. 24 − 31 …”

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confidence: 99%

Collagen-Inspired Helical Peptide Coassembly Forms a Rigid Hydrogel with Twisted Polyproline II Architecture

et al. 2020

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Collagen, the most abundant protein in mammals, possesses notable cohesion and elasticity properties and efficiently induces tissue regeneration. The Gly-Pro-Hyp canonical tripeptide repeating unit of the collagen superhelix has been well-characterized. However, to date, the shortest tripeptide repeat demonstrated to attain a helical conformation contained 3–10 peptide repeats. Here, taking a minimalistic approach, we studied a single repeating unit of collagen in its protected form, Fmoc-Gly-Pro-Hyp. The peptide formed single crystals displaying left-handed polyproline II superhelical packing, as in the native collagen single strand. The crystalline assemblies also display head-to-tail H-bond interactions and an “aromatic zipper” arrangement at the molecular interface. The coassembly of this tripeptide, with Fmoc-Phe-Phe, a well-studied dipeptide hydrogelator, produced twisted helical fibrils with a polyproline II conformation and improved hydrogel mechanical rigidity. The design of these peptides illustrates the possibility to assemble superhelical nanostructures from minimal collagen-inspired peptides with their potential use as functional motifs to introduce a polyproline II conformation into hybrid hydrogel assemblies.

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confidence: 99%

Collagen-Inspired Helical Peptide Coassembly Forms a Rigid Hydrogel with Twisted Polyproline II Architecture

et al. 2020

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“…Such behavior is suggestive of the extremely flexible nature of these bio-filaments. The form-factor modelling approach to analyze the small-angle scattering data for hydrogel-forming systems was demonstrated earlier (Draper et al 2017 ; Fuentes-Caparrós et al 2019 ). These developments could be relevant for Caf1 polymers to some extent, owing to Caf1’s ability to form a hydrogel (Dura et al 2018 ).…”

Section: Resultsmentioning

confidence: 99%

Probing the oligomeric re-assembling of bacterial fimbriae in vitro: a small-angle X-ray scattering and analytical ultracentrifugation study

et al. 2021

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Capsular antigen fragment 1 (Caf1) is an oligomeric protein consisting of 15 kDa monomeric subunits that are non-covalently linked through exceptionally strong and kinetically inert interactions into a linear polymer chain. It has been shown that after its thermal depolymerisation into unfolded monomeric subunits, Caf1 is able to efficiently repolymerise in vitro to reform its polymeric structure. However, little is known about the nature of the repolymerisation process. An improved understanding of this process will lead to the development of methods to better control the lengths of the repolymerised species, and ultimately, to better design of the properties of Caf1-based materials. Here we utilize small-angle X-ray scattering to estimate the size of Caf1 polymers during the first 24 h of the re-polymerisation process. Analytical ultracentrifugation measurements were also used to investigate the process post-24 h, where the rate of repolymerisation becomes considerably slower. Results show that in vitro polymerisation proceeds in a linear manner with no evidence observed for the formation of a lateral polymer network or uncontrolled aggregates. The rate of Caf1 in vitro repolymerisation was found to be concentration-dependent. Importantly, the rate of polymer growth was found to be relatively fast over the first few hours, before continuing at a dramatically slower rate. This observation is not consistent with the previously proposed step-growth mechanism of in vitro polymerisation of Caf1, where a linear increase in polymer length would be expected with time. We speculate how our observations may support the idea that the polymerisation process may be occurring at the ends of the chains with monomers adding sequentially. Our findings will contribute towards the development of new biomaterials for 3D cell culture and bio-printing.

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“…Moreover, the mechanical properties of the gel such as rheology are often dependent on its heating-cooling process. 33,34 There are, however, some rare cases, 35 in which they show a reverse trend. These are so-called heatset gelators that self-assemble at higher temperatures.…”

Section: Heatmentioning

confidence: 99%

Recent Advances on Supramolecular Gels: From Stimuli-Responsive Gels to Co-Assembled and Self-Sorted Systems

Chu

Schalley

2021

Organic Materials

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Gels prepared from low-molecular-weight gelators (LMWGs) represent versatile soft materials. Self-assembly of LMWGs forms nanofibers and above critical gelation concentrations, the entanglement of which leads to self-supporting gels. Owing to the dynamic properties of the self-assembly process, stimuli-responsive LMWGs have prospered in the last decade. In addition, incorporating multiple LMWGs into one system brings the opportunity to achieve sophisticated designs and functions. This review covers recent advances in the field of supramolecular gels, from stimuli-responsive gelators to multicomponent systems that are self-sorting and/or co-assembling.

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Annealing multicomponent supramolecular gels

Abstract: Annealing in a two-component supramolecular gel leads to a self-sorted network, which has significantly different mechanical properties to the as-prepared gels.

Cited by 31 publications

References 44 publications

Collagen-Inspired Helical Peptide Coassembly Forms a Rigid Hydrogel with Twisted Polyproline II Architecture

Collagen-Inspired Helical Peptide Coassembly Forms a Rigid Hydrogel with Twisted Polyproline II Architecture

Probing the oligomeric re-assembling of bacterial fimbriae in vitro: a small-angle X-ray scattering and analytical ultracentrifugation study

Recent Advances on Supramolecular Gels: From Stimuli-Responsive Gels to Co-Assembled and Self-Sorted Systems

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