Annexin A2 and S100A10 in the mammalian oviduct

Abstract

In many mammals upon entry on the female reproductive tract a subpopulation of sperm is stored in the oviduct forming a functional reservoir. In the oviducts of pig and cow, Annexin A2 (AnxA2) has been linked to the binding of sperm. This protein may exist as a monomer or bound to S100A10, and both forms are associated with different biological functions. S100A10 has not yet been reported in the oviduct. The objective of this work was to analyze for the presence of S100A10 in the oviduct and advance in the study of AnxA2 and S100A10 in this organ. This work shows the presence of both proteins, AnxA2 and S100A10, in the oviduct of human, pig, cow, cat, dog and rabbit. At least in pig, AnxA2 is found devoid of S100A10 in the outer surface of the apical plasma membrane of oviductal epithelial cells, indicating it binds to sperm as a monomer or in association with proteins different from S100A10. In the apical cytoplasm of pig oviductal epithelial cells, AnxA2 is associated to S100A10. In primary culture of porcine oviductal cells, the expression of ANXA2 is increased by progesterone, while the expression of S100A10 is increased by progesterone and estradiol. The widespread detection of both proteins in the oviduct of mammals indicates a probable conserved function in this organ. In summary, S100A10 and AnxA2 are widespread in the mammalian oviduct, but AnxA2 would bind sperm in vivo devoid of S100A10 and may be related to reservoir formation. IntroductionThe oviduct is a dynamic organ in which sperm selection, fertilization, and the first stages of embryo development take place. This organ is largely responsible for sperm selection. When spermatozoa enter the oviduct in pigs (Hunter, 1984), cattle (Hunter and Wilmut, 1984), sheep (Hunter et al., 1982), mice (Suarez, 1987), hamsters (Smith and Yanagimachi, 1991), horses (Thomas et al., 1994), dogs (Pacey et al., 2000), cats (Chatdarong et al., 2004), rabbits (Baranda-Avila et al., 2010) and humans (Pacey et al., 1995), a subpopulation of them binds to the epithelial cells forming a reservoir. This process is considered to select for high quality, help control polyspermy, lengthen lifespan, regulate capacitation and select normal sperm (for review see Talevi and Gualtieri, 2010). Sperm binding presents localization differences among diverse species, including sperm retention at the uterus and vagina in rabbit, cat and dog (Barberini et al., 1991, Chatdarong et al., 2004, England et al., 2013. At the oviduct, in vivo, sperm bind predominantly at the crypts of the uterotubal junction (UTJ) and caudal isthmus in sows (Tummaruk et al., 2008), at the caudal isthmus in cattle (Hunter and Wilmut, 1984), and at the ciliated 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 F o r P e e r R e v i e w 3 cells in the distal UTJ in bitches (England et al., 2013). In cats, the UTJ acts as sperm ...

Regulation of the bovine oviductal fluid proteome

“…In this study, a total of 30 and 24 proteins were found to be more abundant at Pre-ov compared with Post-ov in ipsi-and contralateral oviducts respectively. Annexins A1 (ANXA1) and A2 (ANXA2), previously recognized as membrane proteins expressed at the apical side of oviductal epithelial cells in the bovine (Teijeiro et al 2016), were among the most abundant proteins before ovulation. Antibodies directed against annexins A1 and A2 were shown to inhibit the binding of bull sperm to explants of oviductal epithelium, placing these proteins as strong candidates for interaction with spermatozoa in vivo (Ignotz et al 2007).…”

Extracellular Vesicles in the Intrauterine Environment: Challenges and Potential Functions

“…Indeed, proteins of oviductal origin, including oviductin (human) and Annexin A2 and S110A (human and a range of species), are transferred to the sperm cell surface rendering them competent for fertilization [3–6]. It is equally likely that proteins or other mediators, are transferred to sperm during their passage through the uterine cavity.…”

Matrix‐assisted laser desorption/ionization imaging mass spectrometry for the spatial location of feline oviductal proteins

“…) that are able to attach biological structures (Teijeiro et al. ). Annexins have also been related to exocytosis, endocytosis, linkage between membranes or between membranes and the cytoskeleton (Teijeiro et al.…”

“…Recently, Teijeiro et al. () reported the detection of annexin A2 and S100A10 in the oviduct of cow, rabbit, pig, dog and cat oviduct. Likewise, in the present study, the peak that possibly corresponds to annexin A2 was also detected in the cilia of epithelial cells, with different distribution in the infundibulum and ampulla and a minor abundance in the isthmus.…”

“…In the oviduct of sows, S100A10 and annexin A2 in association were found in the apical cytoplasm of epithelial cells, whereas in the outer surface of apical plasma membrane, only annexin A2 was found (Teijeiro et al. ). On the other hand, S100B proteins have never been reported in the oviduct of any species.…”