Annexin A2 Enhances the Progression of Colorectal Cancer and Hepatocarcinoma via Cytoskeleton Structural Rearrangements

Abstract

Annexin A2 (ANXA2) is reported to be associated with cancer development. To investigate the roles ANXA2 plays during the development of cancer, the RNAi method was used to inhibit the ANXA2 expression in caco2 (human colorectal cancer cell line) and SMMC7721 (human hepatocarcinoma cell line) cells. The results showed that when the expression of ANXA2 was efficiently inhibited, the growth and motility of both cell lines were significantly decreased, and the development of the motility relevant microstructures, such as pseudopodia, filopodia, and the polymerization of microfilaments and microtubules were obviously inhibited. The cancer cell apoptosis was enhanced without obvious significance. The possible regulating pathway in the process was also predicted and discussed. Our results suggested that ANXA2 plays important roles in maintaining the malignancy of colorectal and hepatic cancer by enhancing the cell proliferation, motility, and development of the motility associated microstructures of cancer cells based on a possible complicated signal pathway.

Knockdown of Annexin A2 Enhances Radiosensitivity by Increasing G2/M-Phase Arrest, Apoptosis and Activating the p38 MAPK-HSP27 Pathway in Nasopharyngeal Carcinoma

“…These data suggest that ANXA2 overexpression may be a predictor of poor prognosis in NPC patients. Previous studies have shown that ANXA2 overexpression is strongly associated with the progression of multiple malignancies ( 21 – 24 ), and promotes the metastatic potential of endometrial cancer ( 25 , 26 ), kidney cancer ( 27 , 28 ) and colorectal cancer ( 29 , 30 ). A recent meta-analysis to evaluate the prognostic value of ANXA2 overexpression in malignant tumors revealed that ANXA2 overexpression correlated with poor outcomes in patients with malignant tumors ( 31 ).…”

TAGLN2 promotes the proliferation, invasion, migration and epithelial‑mesenchymal transition of colorectal cancer cells by activating STAT3 signaling through ANXA2

“…The calponin homolog domain contained within the TAGLN2 protein structure implicates the possible involvement of TAGLN2 in cytoskeletal reorganization, and TAGLN2 was shown to be directly and indirectly involved in numerous cancer-related processes, such as migration, proliferation, differentiation or apoptosis ( 19 , 34 ). Previous studies reported a relationship between TAGLN2 expression and cancer ( 33 , 35 ).…”

“…High expression of ANXA2 has been shown to be associated with the progression of invasion, metastasis, and angiogenesis in cancer ( 17 ). Notably, multiple studies have elucidated that ANXA2 is expressed at higher levels in CRC than in normal colorectal tissues and is associated with malignancy in CRC, as evidenced by alterations associated with cell proliferation, motility progression, recurrence and survival ( 18 , 19 ), suggesting a significant and non-negligible role for ANXA2 in tumor treatment and prognosis.…”

Effect and mechanism of action of SLP‑2 on the apoptosis and autophagy of gastric cancer cells

“…ANXA2 is a multifunctional calcium-dependent phospholipid-binding protein that is widely distributed in the membrane, cytoplasm and nucleus of eukaryotic cells (29). ANXA2 plays important roles in cytoskeleton remodeling, cell phenotype change and cell movement by regulating the expression of connexin molecules (30)(31)(32). The abnormal expression of ANXA2 is closely associated type 2 diabetes, cancer and autoimmune diseases (33)(34)(35).…”