Annexin A4 and cancer

Wei, Bin; Guo, Chunmei; Liu, Shuqing; Sun, Ming‐Zhong

doi:10.1016/j.cca.2015.05.016

Cited by 51 publications

(35 citation statements)

References 67 publications

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“…Annexins are also involved in a wide variety of pathological conditions ranging from cancer (i.e. annexins A2, A4 and several others) ( Mussunoor and Murray, 2008;Wang and Lin, 2014;Wei et al, 2015), resistance to chemotherapy [annexin A13 (AnxA13)] (Reiske et al, 2010) to inflammation and autoimmunity (i.e. annexin A1, A2 or A5) (D'Acquisto et al, 2013;Misasi et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

Structural and lipid-binding characterization of human annexin A13a reveals strong differences with its long A13b isoform

Fernández‐Lizarbe

Lecona

Santiago-Gómez

et al. 2016

Biological Chemistry

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Annexin A13 is the founder member of the vertebrate family of annexins, which are comprised of a tetrad of unique conserved domains responsible for calcium-dependent binding to membranes. Its expression is restricted to epithelial intestinal and kidney cells. Alternative splicing in the N-terminal region generates two isoforms, A13a and A13b, differing in a deletion of 41 residues in the former. We have confirmed the expression of both isoforms in human colon adenocarcinoma cells at the mRNA and protein levels. We have cloned, expressed, and purified human annexin A13a for the first time to analyze its structural characteristics. Its secondary structure and thermal stability differs greatly from the A13b isoform. The only tryptophan residue (Trp 186 ) is buried in the protein core in the absence of calcium but is exposed to the solvent after calcium binding even though circular dichroism spectra are quite similar. Non-myristoylated annexin A13a binds in a calcium-dependent manner to acidic phospholipids but not to neutral or raft-like liposomes. Calcium requirements for binding to phosphatidylserine are around 6-fold lower than those required by the A13b isoform. This fact could account for the different subcellular localization of both annexins as binding to basolateral membranes seems to be calcium-dependent and myristoylation-independent.

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Section: Introductionmentioning

confidence: 99%

Structural and lipid-binding characterization of human annexin A13a reveals strong differences with its long A13b isoform

Fernández‐Lizarbe

Lecona

Santiago-Gómez

et al. 2016

Biological Chemistry

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“…[60, 61] Annexin 4 while typically found over expressed in most tumors was found to have the opposite relationship in prostate cancer. [62, 63] HSP70 expression is also higher in thymoma B3 than A, consistent with its activity promoting cell survival. [64, 65] We found that NFKB1, which controls genes associated with processes such as apoptosis inhibition and cell cycle progression, was expressed significantly higher in B3 thymoma.…”

Section: Discussionmentioning

confidence: 81%

“…[69, 70] High levels of proteins HSP90b1 and αB-crystallin are typically associated with cancer progression. [62, 71–73] Surprisingly, these proteins were found to be significantly more highly expressed in thymoma A over thymoma B3, despite B3 being the more severe disease. Differences in the site of origin of thymoma A and B3 may play a role in these unexpected results.…”

Section: Discussionmentioning

confidence: 99%

Proteomic Signatures of Thymomas

et al. 2016

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Based on the histological features and outcome, the current WHO classification separates thymomas into A, AB, B1, B2 and B3 subtypes. It is hypothesized that the type A thymomas are derived from the thymic medulla while the type B thymomas are derived from the cortex. Due to occasional histological overlap between the tumor subtypes creating difficulties in their separation, the aim of this study was to provide their proteomic characterization and identify potential immunohistochemical markers aiding in tissue diagnosis. Pair-wise comparison of neoplastic and normal thymus by liquid chromatography tandem mass spectrometry (LC-MS/MS) of formalin fixed paraffin embedded tissue revealed 61 proteins differentially expressed in thymomas compared to normal tissue. Hierarchical clustering showed distinct segregation of subtypes AB, B1 and B2 from that of A and B3. Most notably, desmoyokin, a protein that is encoded by the AHNAK gene, was associated with type A thymomas and medulla of normal thymus, by LC-MS/MS and immunohistochemistry. In this global proteomic characterization of the thymoma, several proteins unique to different thymic compartments and thymoma subtypes were identified. Among differentially expressed proteins, desmoyokin is a marker specific for thymic medulla and is potentially promising immunohistochemical marker in separation of type A and B3 thymomas.

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“…Family members including annexin A1, A2, A4, A5, A7, A10, and A11 have been suggested to be associated with specific clinicopathological characteristics, including metastatic status, in CRC and other cancers [8–16]. Surprisingly, ANXA13, which is highly expressed in the digestive system and believed to be the original founder gene of the annexin A family, has not been studied as a possible biomarker in CRC [17, 18].…”

Section: Introductionmentioning

confidence: 99%

Annexin A13 promotes tumor cell invasion in vitro and is associated with metastasis in human colorectal cancer

Jiang

Wang

et al. 2017

Oncotarget

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PurposeAberrantly upregulated expression of selected members of annexin, a group of calcium- and membrane-binding proteins, have been found to be associated with metastasis, poor prognosis, and other clinical characteristics in colorectal cancer (CRC), the third most diagnosed cancer. However, ANXA13 (encoding protein annexin A13), the original founder gene of the annexin A family, has not been studied carefully as a potential prognostic biomarker in CRC.MethodsThe protein level of annexin A13 was determined by western blot in a panel of CRC cell lines. Tumor cell invasion was determined by a Matrigel in vitro invasion assay in selected CRC cells with either upregulated (via plasmid transfection) or downregulated (via siRNA treatment) expression of ANXA13. The clinicopathological features and prognostic values associated with ANXA13 expression were also evaluated in a group of 125 CRC patients.ResultsANXA13 was expressed at a high level in HCT116 and HT29 cells but undetected or at a lower level in SW620, SW48, and Rko cells. CRC cell invasion was promoted by ANXA13 overexpression in SW620 or Rko cells and was reduced by ANXA13 downregulation in HCT116 or HT29 cells. In CRC patients, ANXA13 expression levels correlated with lymph node metastasis and were associated with poor overall survival.ConclusionsANXA13 is associated with CRC cell invasion in vitro, and with lymph node metastasis and poor survival in CRC patients. Our results indicate that ANXA13 can be exploited as a biomarker for its diagnostic and prognostic values.

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Annexin A4 and cancer

Cited by 51 publications

References 67 publications

Structural and lipid-binding characterization of human annexin A13a reveals strong differences with its long A13b isoform

Structural and lipid-binding characterization of human annexin A13a reveals strong differences with its long A13b isoform

Proteomic Signatures of Thymomas

Annexin A13 promotes tumor cell invasion in vitro and is associated with metastasis in human colorectal cancer

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