Annona muricata assisted biogenic synthesis of silver nanoparticles regulates cell cycle arrest in NSCLC cell lines

Meenakshisundaram, Shanmugapriya; Varunkumar, Krishnamoorthy; Jagadeesan, Yogeswaran; Ravikumar, Vasulinga T.; Balaiah, Anandaraj

doi:10.1016/j.bioorg.2019.103451

Cited by 36 publications

(15 citation statements)

References 56 publications

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“…Furthermore, the AgNPs did not induce caspase-3 enzyme (result not shown), the final executioner enzyme for the induction of apoptosis. This is contrary to the previous finding that reported activation of apoptosis of the nanoparticles in NSCLC cell lines using different assay apart from the caspase-3 induction [29]. This implies either that the cells had already gone beyond apoptosis stage at the period of evaluation or the HaCaT cell line is not sensitive to the apoptotic effect induced by the nanoparticles.…”

Section: Cytotoxicity Potential Of Synthesized Agnpscontrasting

confidence: 98%

“…The aggregation in synthesized nanoparticles induces instability in the particles and hence obliterates its essential functional properties [42]. The DLS, PDI and zeta potential values of the work of Meenakshisundaram et al [29] and Gavamukulya et al [16] are different from the values obtained in this study. The disparity in values is likely to be due to the differences in the preparation of the nanoparticles.…”

Section: Physicochemical Characterization Of Synthesized Agnpscontrasting

confidence: 75%

“…Some of these phytochemicals have been shown to be responsible for the formation of nanoparticles by causing the excitation of surface plasmon resonance (SPR) of the particles [26,27,28]. Photo induced synthesis of the AgNPs as presented in this study has shown that introduction of heat is not necessary as reported by previous studies [15,29].…”

Section: Photo Aided Synthesis Of Annona Muricata-agnpssupporting

confidence: 66%

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Photo-assisted bio-fabrication of silver nanoparticles using Annona muricata leaf extract: exploring the antioxidant, anti-diabetic, antimicrobial, and cytotoxic activities

Badmus

Oyemomi

Adedosu

et al. 2020

Heliyon

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Green synthesis of metal nanoparticles is reputed to have a robust range of biomedical applications. Silver nanoparticles (AgNPs) bio-fabricated using aqueous leaf extract of Annona muricata were characterized and evaluated for in-vitro antioxidant, lipid peroxidation inhibition, anti-diabetic and antimicrobial activities as well as cytotoxicity in human keratinocyte cells (HaCaT).The extract induced colour change of silver salt solution which absorbed at 420 nm and confirmed the formation of AgNPs. FTIR showed that free amide and hydroxyl groups were responsible for the synthesized nanoparticles. Both XRD and SAED confirmed the crystalline nature of the particles with face centered cubic (FCC) phase. The zeta potential revealed -27.2 mV potential and average distribution size of 35 nm. DLS indicated that the majority of the particles were 86.78 nm size and with a polydispersity index (PDI) of 0.329.AgNPs displayed strong activities against DPPH (IC 50 ¼ 51.80 μg/ml), ABTS (IC 50 ¼ 30.78 μg/ml), α-amylase (IC 50 ¼ 0.90 μg/ml) and α-glucosidase (IC 50 ¼ 3.32 μg/ml). The particles exhibited a dose-dependent inhibition of Fe 2þ -induced lipid peroxidation with effective antimicrobial activity against a battery of bacterial strains and cytotoxicity in HaCaT cell line. These findings revealed the potential biomedical applications of the particles and further work will be required to establish its molecular mechanism of action.

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Section: Cytotoxicity Potential Of Synthesized Agnpscontrasting

confidence: 98%

Section: Physicochemical Characterization Of Synthesized Agnpscontrasting

confidence: 75%

Section: Photo Aided Synthesis Of Annona Muricata-agnpssupporting

confidence: 66%

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Photo-assisted bio-fabrication of silver nanoparticles using Annona muricata leaf extract: exploring the antioxidant, anti-diabetic, antimicrobial, and cytotoxic activities

Badmus

Oyemomi

Adedosu

et al. 2020

Heliyon

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“…A previous study 43 has confirmed an efficient induction of apoptotic protein expression in lung carcinoma cancer cell line via silver nanoparticle of soursop aqueous leaf extract. The programmed cell death involved mitochondrial-mediated intrinsic pathways.…”

Section: Analysis Of Apoptosis and Necrosis By Flowcytometrymentioning

confidence: 76%

Single-Dose and Combined-Dose of Nanoparticles from Soursop Leaves (Annona muricata L.) and Sappan Wood (Caesalpinia sappan L.) Induced Apoptosis and Necrosis in HeLA Cells

Astirin¹,

Prayitno²,

Artanti³

et al. 2021

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Introduction: Apart from the medical advancement of chemotherapy, various plants were known as beneficial for cancer therapy because they can kill cancer cells selectively without damaging the normal cells. Here, we showed that nanoparticles formulated from chloroform fraction of soursop (Annona muricata L.) leaves and ethyl acetate fraction of sappan wood (Caesalpinia sappan L.) have anti-proliferative and cytotoxic effects on HeLa cervical cancer cells. Methods: The cytotoxic effect was evaluated using a single dose of each nanoparticle and a combined dose to obtain a synergistic effect. The mechanism of induced cell death via apoptosis or necrosis pathway was evaluated using flow cytometry by incorporating Annexin V and propidium iodide. Results: Synthesis of nanoparticles from the extract of soursop leaves (nano-SL) and extract of sappan wood (nano-SW) yielded particle sizes ranging from 248 to 317 nm. Nano-SL and nano-SW decreased the viability of HeLa cervical cancer cells in a dose-dependent manner with IC 50 values of 63,32 μg/ml dan 40,88 μg/ml, respectively. The combined dose of 1/8 IC 50 from both nanoparticles showed a strong synergistic effect, as shown by the combination index value of 0.13 based on the same mode of action and different modes of action. In HeLa cells treated with a combined dose of nanoparticles, the total apoptotic cells increased two times greater than that in control cells. Conclusion:Nano-SL and nano-SW induce apoptosis and necrosis in HeLa cells. Combined-dose of both nanoparticles produced a synergistic effect that could reduce the amount of the required individual dose while increasing the total effect.

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“…Meenakshisundaram et al performed a green synthesis of AgNPs from Annona muricata leaf extract and studied their growth‐inhibitory activity against non‐small cell lung cancer cells. Their findings indicated that the prepared AgNPs arrest the cell cycle at the G0‐G1 phase, where the cell's viability is higher in the treated group than in the untreated group (Meenakshisundaram et al, 2020).…”

Section: Nps Types and Applicationmentioning

confidence: 99%

Application of nanoparticles in cancer therapy with an emphasis on cell cycle

Almanghadim

Nourollahzadeh

Khademi

et al. 2021

Cell Biology International

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Owing to their unique characteristics, nanoparticles (NPs) could be incorporated into valuable therapeutic modalities for different diseases; however, there are many concerns about risk factors in human applications. NPs carry therapeutic chemicals that could improve the outcome of cancer therapies. Nowadays, NPs are being recognized as important and strategic agents in treatment of several disorders due to their unique properties in targeting malignant cells in tumor sites. Numerous investigations have shown that the majority of chemotherapeutic agents can be modified through entrapment in submicron colloidal systems. Still, there are problems and limitations in application of NPs in cancer therapy. The aim of the present study is to focus on potential NPs usage in cancer treatment with an emphasis on the cell cycle of malignant cells.

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Annona muricata assisted biogenic synthesis of silver nanoparticles regulates cell cycle arrest in NSCLC cell lines

Cited by 36 publications

References 56 publications

Photo-assisted bio-fabrication of silver nanoparticles using Annona muricata leaf extract: exploring the antioxidant, anti-diabetic, antimicrobial, and cytotoxic activities

Photo-assisted bio-fabrication of silver nanoparticles using Annona muricata leaf extract: exploring the antioxidant, anti-diabetic, antimicrobial, and cytotoxic activities

Single-Dose and Combined-Dose of Nanoparticles from Soursop Leaves (Annona muricata L.) and Sappan Wood (Caesalpinia sappan L.) Induced Apoptosis and Necrosis in HeLA Cells

Application of nanoparticles in cancer therapy with an emphasis on cell cycle

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