Annotated expressed sequence tags and xenobiotic detoxification in the aphid Myzus persicae (Sulzer)

Abstract

Aphids (Hemiptera: Aphididae) are phytophagous insects that are important agricultural pests. The enormous negative economic impacts caused by aphids worldwide are well known, and are mostly due to their high multiplication rate and the transmission of phytopathogenic viruses. Aphid management strategies mainly involve chemical treatments which are pollutants and are increasingly inefficient, since aphids have developed multiple insecticide-resistant mechanisms. Among the most economically important species is the green peach aphid Myzus persicae Sulzer (Aphididae: Macrosiphini), which is able to colonize a wide range of host plants belonging to many different families, and transmits numerous plant viruses. Because of its large prevalence, M. persicae has been the target of massive insecticide treatments; consequently, it has evolved several insecticide-resistant mechanisms. In this work, a collection of expressed genes from M. persicae is presented in order to identify putative genes involved in xenobiotic detoxification. After cDNA cloning and sequencing, 959 expressed sequence tags (EST) were annotated. Most sequences matched known genes corresponded to metabolism proteins (26%), ribosomal proteins (23%) and structural proteins (8%). Among them, several sequences corresponded to proteins putatively involved in sensing, degradation or detoxification of plant xenobiotic products.

Sex versus parthenogenesis: A transcriptomic approach of photoperiod response in the model aphid Acyrthosiphon pisum (Hemiptera: Aphididae)

“…Some cuticular as well as some signalling proteins have been shown to be regulated by the photoperiod in the pea aphid (Le Trionnaire et al, 2007). Recently, in the frame of the International Aphid Genomic Consortium, a large number of expressed sequence tags (ESTs) have been obtained from standard cDNA libraries (Hunter et al, 2003;Tagu et al, 2004;Figueroa et al, 2007), with a particular effort on the pea aphid (A. pisum) (SabaterMuñoz et al, 2006) for which the full assembled genome sequence will be soon available.…”

Comparative analysis of the Acyrthosiphon pisum genome and expressed sequence tag‐based gene sets from other aphid species

“…With regard to other aphid species, different collections of ESTs have been obtained in Myzus persicae (tribe: Macrosiphini) (Figueroa et al, 2007;Ramsey & Al, 2007) and also in two species of the Aphidini tribe, Toxoptera citricida and Aphis gossypii (Hunter et al, 2003). Most of these ESTs have already been described in order to identify organ-specific expression patterns and, in particular, the genes involved in feeding.…”

The secreted salivary proteome of the pea aphid Acyrthosiphon pisum characterised by mass spectrometry

“…In particular, EST libraries have been constructed for several species [11,12], and the full genome of the pea aphid Acyrthosiphon pisum has recently been sequenced and assembled, with the annotation in progress (http://genouest.org/cgi-bin/gbrowse/gbrowse/aphidbase). Furthermore, the essential role of one gene expressed in the salivary glands has been revealed by the premature death of plant-reared pea aphids in which the transcript of this gene was selectively suppressed by RNAi [13].…”