Annotation and characterization of Cd-responsive metal transporter genes in rapeseed (Brassica napus)

Zhang, Xian Duo; Meng, Jin Guo; Zhao, Kai; Chen, Xi; Yang, Zhi Min

doi:10.1007/s10534-017-0072-4

Cited by 51 publications

(19 citation statements)

References 57 publications

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“…Alternatively, some plants that accumulate a high level of Cd in shoots may show a low level of Cd in roots. In this regard, the highly efficient transport of Cd from roots to shoots occurred through some specific metal transporters such as OsHMA2 (P‐Type Heavy Metal ATPase) ( Takahashi et al, ) and other transporter families ( Zhang et al, ). Nevertheless, a recent study has shown that a rapeseed genotype L338 had a higher capacity for Cd uptake by roots but a lower Cd concentration in shoots when compared to the control (genotype L351; Wu et al, ).…”

Section: Discussionmentioning

confidence: 99%

Isolation and identification of rapeseed (Brassica napus) cultivars for potential higher and lower Cd accumulation

Zhang

Wang

et al. 2018

J. Plant Nutr. Soil Sci.

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Cadmium (Cd) as a non-essential toxic metal has become one of the seriously environmental problems. Overload of Cd into plant shoots, particularly the addible parts (i.e., grains), jeopardizes crop production and food safety. Isolating and identifying genotypic variations in Cd accumulation of rapeseed (Brassica napus) cultivars is an efficient approach for phytoremediation and developing lower Cd-accumulating plants. In this study, a trial was conducted under natural condition in Nanjing, China, from 2014 to 2017, and identified 64 rapeseed cultivars collected from the areas of Gui Zhou province. Rapeseed grew under moderate Cd exposure (5 mg kg -1 ) for 5 months, and shoots were harvested for Cd quantification. A great variation of total Cd concentrations in shoots, ranking from 0.16 to 17.03 mg Cd kg -1 , was found. Following the initial examination of all cultivars, two sets of plants with high (#138 and #177) and low (#208 and #244) Cd concentrations were further investigated. Throughout the growth period, cultivars #138 and #177 accumulated more Cd during vegetative (30, 60, and 120 d) and late developmental (180 d) stages than cultivars #208 and #244. The higher Cd concentration in shoots of #138 and #177 was associated with the higher Cd concentration in xylem sap, suggesting the greater capability of Cd translocation from roots to shoots. Compared to #208 and #244, Cd exposure moderately reduced zinc and iron concentrations in some tissues of #138 and #177, whereas the manganese and magnesium concentrations showed no change. Although #138 and #177 cultivars accumulated more Cd in their shoots, no Cd toxicity was detected. Moreover, both #138 and #177 cultivars had a similar biomass to #208 or #244. These results suggest that #138 and #177 rapeseeds are tolerant to Cd stress.

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Section: Discussionmentioning

confidence: 99%

Isolation and identification of rapeseed (Brassica napus) cultivars for potential higher and lower Cd accumulation

Zhang

Wang

et al. 2018

J. Plant Nutr. Soil Sci.

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“…Metal homeostasis is a mechanism essential for plant growth, development and adaptation to diverse environmental stresses [1–3]. It is built up by metal uptake and transport across cells or within the cells, which largely relies on a variety of metal transporters [4, 5]. The plant ZIP ( Z n-regulated transporter, I ron-regulated transporter-like P rotein) proteins belong to the metal transporter family involved in uptake and allocation of Zn, Cu, Cd, iron (Fe) or manganese (Mn) [1, 2, 6–8].…”

Section: Introductionmentioning

confidence: 99%

OsZIP1 functions as a metal efflux transporter limiting excess zinc, copper and cadmium accumulation in rice

et al. 2019

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Background Metal homeostasis is critical for plant growth, development and adaptation to environmental stresses and largely governed by a variety of metal transporters. The plant ZIP ( Z n-regulated transporter, I ron-regulated transporter-like P rotein) family proteins belong to the integral membrane transporters responsible for uptake and allocation of essential and non-essential metals. However, whether the ZIP family members mediate metal efflux and its regulatory mechanism remains unknown. Results In this report, we provided evidence that OsZIP1 is a metal-detoxified transporter through preventing excess Zn, Cu and Cd accumulation in rice. OsZIP1 is abundantly expressed in roots throughout the life span and sufficiently induced by excess Zn, Cu and Cd but not by Mn and Fe at transcriptional and translational levels. Expression of OsZIP-GFP fusion in rice protoplasts and tobacco leaves shows that OsZIP1 resides in the endoplasmic reticulum (ER) and plasma membrane (PM). The yeast ( Saccharomyces cerevisiae ) complementation test shows that expression of OsZIP1 reduced Zn accumulation. Transgenic rice overexpressing OsZIP1 grew better under excess metal stress but accumulated less of the metals in plants. In contrast, both oszip1 mutant and RNA interference (RNAi) lines accumulated more metal in roots and contributed to metal sensitive phenotypes. These results suggest OsZIP1 is able to function as a metal exporter in rice when Zn, Cu and Cd are excess in environment. We further identified the DNA methylation of histone H3K9me2 of OsZIP1 and found that OsZIP1 locus, whose transcribed regions imbed a 242 bp sequence, is demethylated, suggesting that epigenetic modification is likely associated with OsZIP1 function under Cd stress. Conclusion OsZIP1 is a transporter that is required for detoxification of excess Zn, Cu and Cd in rice. Electronic supplementary material The online version of this article (10.1186/s12870-019-1899-3) contains supplementary material, which is available to authorized users.

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“…They play a significant role in abiotic stress response as they control the traffic of ions and other biomolecules such as hormones and compatible solutes during stress to sustain vital cellular processes such as ion homeostasis, osmotic adjustment, signal transduction, and detoxification (Vishwakarma et al, 2019). Several transporter gene families ( Table 1) have been identified and characterized in B. napus such as aquaporins (Yuan et al, 2017), metal transporter genes [MTGs;Zhang et al (2018a)], ATP-binding cassette (ABC) transporter (Zhang et al, 2018b), sucrose transporters or sucrose carriers (SUT/ SUCs) and Sugars Will Eventually be Exported Transporters [SWEET;Jian et al (2016a)]. Most of these gene families are involved in response to drought, salt, low/high temperatures, heavy metal, and hormone treatments.…”

Section: Transportersmentioning

confidence: 99%

Engineering Multiple Abiotic Stress Tolerance in Canola, Brassica napus

et al. 2020

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Impacts of climate change like global warming, drought, flooding, and other extreme events are posing severe challenges to global crop production. Contribution of Brassica napus towards the oilseed industry makes it an essential component of international trade and agroeconomics. Consequences from increasing occurrences of multiple abiotic stresses on this crop are leading to agroeconomic losses making it vital to endow B. napus crop with an ability to survive and maintain yield when faced with simultaneous exposure to multiple abiotic stresses. For an improved understanding of the stress sensing machinery, there is a need for analyzing regulatory pathways of multiple stressresponsive genes and other regulatory elements such as non-coding RNAs. However, our understanding of these pathways and their interactions in B. napus is far from complete. This review outlines the current knowledge of stress-responsive genes and their role in imparting multiple stress tolerance in B. napus. Analysis of network cross-talk through omics data mining is now making it possible to unravel the underlying complexity required for stress sensing and signaling in plants. Novel biotechnological approaches such as transgene-free genome editing and utilization of nanoparticles as gene delivery tools are also discussed. These can contribute to providing solutions for developing climate change resilient B. napus varieties with reduced regulatory limitations. The potential ability of synthetic biology to engineer and modify networks through fine-tuning of stress regulatory elements for plant responses to stress adaption is also highlighted.

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Annotation and characterization of Cd-responsive metal transporter genes in rapeseed (Brassica napus)

Cited by 51 publications

References 57 publications

Isolation and identification of rapeseed (Brassica napus) cultivars for potential higher and lower Cd accumulation

Isolation and identification of rapeseed (Brassica napus) cultivars for potential higher and lower Cd accumulation

OsZIP1 functions as a metal efflux transporter limiting excess zinc, copper and cadmium accumulation in rice

Engineering Multiple Abiotic Stress Tolerance in Canola, Brassica napus

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