Anomalias meióticas de oócitos de pacientes com endometriose submetidas à estimulação ovariana

Abstract

the present study data did not show significant differences in the IVM or in the meiotic anomalies rate between the IVM oocytes from stimulated cycles of patients with endometriosis, as compared with controls. Nevertheless, they have suggested a delay in the outcome of oocyte meiosis I from patients with endometriosis, shown by the higher proportion of oocytes in telophase I observed in this group.

Recurrent germinal vesicle resistant to in vitro maturation: case report

“…A descoberta da expressão significativa da Areg no fluido folicular humano (Inoue et al, 2009) no caso em debate há que se considerar ainda a interferência da endometriose sobre a maturação in vivo dos gametas. Um grupo de pesquisadores brasileiros analisou por microscopia de fluorescência as características do fuso celular e cromossômicas dos oócitos classificados morfologicamente como MII de pacientes com endometriose e controles (fatores tubário e/ou masculino de infertilidade) após MIV e constatou tendência à ocorrência de proporção superior de oócitos em telófase I no grupo com endometriose (27,8% versus 9,1%), sugerindo que oócitos considerados maduros por critérios morfológicos, podem não ter concluído a primeira divisão meiótica (Barcelos et al, 2008;Barcelos et al, 2009). Diante do que foi exposto, fica claro que, apesar dos avanços na técnica de MIV, com registros de sucesso de ciclos, ainda existem lacunas referentes ao seu entendimento.…”

Peritoneal Fluid From Infertile Women With Minimal/Mild Endometriosis Compromises the Meiotic Spindle of Metaphase II Bovine Oocytes: A Pilot Study

“…The meiotic cell spindle of human oocytes in metaphase II (MII) is a temporary and dynamic structure consisting of microtubules, which are relevant to ensuring chromosome segregation during the meiotic process. 16,17 It is very sensitive to many factors, including oxidative stress (OS), [18][19][20] which results from a change in the balance of oxidants/antioxidants 2 and may cause meiotic abnormalities, chromosome instability, apoptosis, and impairment of preimplantation embryo development. 21,22 Some authors have associated endometriosis with OS, 5,23,24 suggesting that pelvic endometriosis is possibly related to an inflammatory response to ectopic endometrial implants, 25 promoting the loss of redox equilibrium and OS in the pelvic microenvironment.…”

“…The meiotic cell spindle of human oocytes in metaphase II (MII) is a temporary and dynamic structure consisting of microtubules, which are relevant to ensuring chromosome segregation during the meiotic process. 16,17 It is very sensitive to many factors, including oxidative stress (OS), 18 –20 which results from a change in the balance of oxidants/antioxidants 2 and may cause meiotic abnormalities, chromosome instability, apoptosis, and impairment of preimplantation embryo development. 21,22…”

O fluido peritoneal de mulheres inférteis com endometriose mínima/leve compromete o fuso meiótico de oócitos bovinos em metáfase II

“…A qualidade ooc m maturação citoplasmática e nuclear (FERREIRA et al, 2009), sendo que a última dependente da presença de um fuso celular normal. O fuso celular meiótico de oócitos em metáfase II (MII) é uma estrutura temporária e dinânima, em forma de barril, composta por microtúbulos formados por dímeros de tubulina que se polimerizam e despolimerizam nos diversos estágios do ciclo celular, sendo relevante para assegurar a segregação cromossômica durante o processo meiótico (CHOI et al, 2007;BARCELOS et al, 2008BARCELOS et al, , 2009. O fuso é bastante sensível a ações de diversos fatores, entre eles o estresse oxidativo (EO) (HU et al, 2001;MULLEN et al, 2004), capaz de causar anomalias meióticas, instabilidade cromossômica, indução de apoptose e comprometimento do desenvolvimento embrionário pré-implantação (NAVARRO et al, , 2006.…”

“…Oocyte quality depends on appropriate cytoplasmic and nuclear maturation (Ferreira et al, 2009), and the latter depends on the presence of a normal cell spindle. The meiotic cell spindle of human oocytes in metaphase II (MII) is a temporary and dynamic structure consisting of microtubules, which are relevant to ensuring chromosome segregation during the meiotic process (Barcelos et al, 2008(Barcelos et al, , 2009. The cell spindle of the oocyte is very sensitive to many factors, among them oxidative stress (OS) (Hu et al, 2001;Mullen et al, 2004), which results from a change in the balance of oxidants/antioxidants (Gupta et al, 2006) and is able to cause meiotic abnormalities, chromosome instability, induction of apoptosis, and impairment of preimplantation embryo development (Navarro et al, , 2006; it may also be involved in the etiopathogenesis of endometriosis-related infertility (Campos Petean et al, 2008;Gupta et al, 2008).…”