Anorganic Bovine Bone Supports Osteoblastic Cell Attachment and Proliferation

Stephan, Eugena B.; Jiang, Di; Lynch, Samuel E.; Bush, Peter J.; Dziak, Rosemary

doi:10.1902/jop.1999.70.4.364

Cited by 60 publications

(53 citation statements)

References 18 publications

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“…34,35 Our findings supplement and extend a recent report on the early (up to 24 h) attachment and proliferation of neonatal rat calvaria cells on the same natural bone mineral (Bio-Oss) in particulate form. 36 We observed the formation and accumulation of mature collagen fibrils. This reflects the complete processing of procollagen to mature collagen ␣-chains, including the formation of two covalent nonreducible crosslinks (HP and LP) which are formed by a sequence of posttranslational modifications.…”

Section: Discussionmentioning

confidence: 89%

“…[26][27][28][29][30][31][32][33][34][35][36] In the present study, we investigated the attachment, proliferation, and matrix synthesis of human osteoblast-like cells onto highly porous natural bone mineral (Bio-Oss) by SEM, TEM, and biochemical analysis. SEM and TEM analysis after 6 weeks revealed that osteoblast-like cells were able to colonize the biomaterial and to synthesize collagen.…”

Section: Discussionmentioning

confidence: 99%

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Three-dimensional cultivation of human osteoblast-like cells on highly porous natural bone mineral

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Terheyden

Dunsche

et al. 2000

J. Biomed. Mater. Res.

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In this study, we investigated the growth and extracellular matrix synthesis of human osteoblast-like cells on highly porous natural bone mineral. Human bone cells were isolated from trabecular bone during routine iliac crest biopsies. Under conventional culture conditions, trabecular bone cells were able to assume the organization of a three-dimensional structure on a porous natural bone mineral (Bio-Oss(R) Block). Scanning electron microscopy examination after 6 weeks revealed multiple cell layers on the trabecular block. Transmission electron microscopy examination after 6 weeks revealed the accumulation of mature collagen fibrils in the intracellular and extracellular spaces, and showed multilayered, rough endoplasmic reticulum as well as mitochondria-rich cells surrounded by dense extracellular matrix. These morphological observations suggest that the cell layer may resemble the natural three-dimensional structure. Biochemical analysis revealed that the hydroxylysylpyridinoline, lysylpyridinoline, and hydroxyproline content of the cell layer increased in a time-dependent manner, whereas in monolayer culture without natural bone mineral, no measurable amounts of hydroxylysylpyridinoline or lysylpyridinoline, and a barely measurable amount of hydroxyproline, were noted. Mature collagen extracted by ethylenediaminetetraacetic acid-demineralization from the cell layer on natural bone mineral showed an identical electrophoretic pattern to that observed in human bone, as evaluated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The present study demonstrated an excellent biocompatibility of the highly porous natural bone mineral in a three-dimensional bone cell culture system, and thus its potential for tissue-engineered growth of human bone.

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Section: Discussionmentioning

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Three-dimensional cultivation of human osteoblast-like cells on highly porous natural bone mineral

Açil

Terheyden

Dunsche

et al. 2000

J. Biomed. Mater. Res.

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“…poly(P) stimulates bFGF-induced cell proliferation and vascularization, and these effects resulted in a significantly higher BIC in the Poly(P)/IP-CHA group than the IP-CHA group. A bovine-derived anorganic bone matrix such a Bio-Oss ® has been reported superior bone formation and provides scaffold for osteoblasts as an alternative to autologous bone [20][21][22] .…”

Section: Discussionmentioning

confidence: 99%

Inorganic polyphosphate adsorbed onto hydroxyapatite for guided bone regeneration: An animal study

et al. 2014

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Inorganic polyphosphate (poly(P)) is recognized as a therapeutic agent that promotes fibroblast growth factor and enhances osteogenic differentiation, and in vivo, when adsorbed onto interconnected porous calcium hydroxyapatite (IP-CHA) enhances bone regeneration. The present study focused on the effect of poly(P) adsorbed onto IP-CHA granules (Poly(P)/IP-CHA) in guided bone regeneration (GBR). Dental implants were placed into the edentulous mandibular areas of five Beagle-Labrador hybrid dogs with screw expose on the buccal side, and then bone defects were filled Poly(P)/IP-CHA (test) or IP-CHA (control). After 12 weeks, histological evaluation and histomorphometrical analysis were performed. Newly-bone formation around exposed implant screw was clearly detected in the test-group. The ratio for regenerated bone height in the test group versus the control-group was 85.6±20.2 and 62.6±23.8, respectively, with no significant difference, while, that for bone implant contact was significantly higher (67.9±11.8 and 48.8±14.1, respectively). These findings indicate that Poly(P)/IP-CHA enhances bone regeneration in GBR.

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“…Some of these xenografts further display an architectural organization with interconnected pores and biomechanical resistance similar to those of human bone (Stephan 1999). …”

Section: Introductionmentioning

confidence: 98%

Expression of Metalloproteinase 2 in the Cell Response to Porous Demineralized Bovine Bone Matrix

et al. 2005

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The purpose of the study was to analyze the involvement of metalloproteinase 2 (MMP-2) and macrophages in the tissue and cell response to the organic graft material produced from bovine cancellous bone. Thirty adult male white Wistar rats (Rattus norvegicus) received implants of blocks of demineralized bovine bone matrix between the fasciae of the quadriceps muscle. The specimens collected at 3, 7, 14, 21 and 28 days after implantation (n = 6/period). Sections of 6 microm thick were stained with hematoxylin and eosin and immunolabeled with anti-MMP-2 and anti-CD68 using standard avidin-biotin-peroxidase method. The tissue response to the material was initially mediated by polymorphonuclear neutrophils, evolving to a mononuclear inflammatory infiltrate with macrophages and few lymphocytes and plasma cells and presence of inflammatory multinucleated giant cells (GC) in contact with the material that exhibited signs of resorption. The number of cells immunolabeled to MMP-2 was highest at day 7 (103.2 +/- 39.1), but significantly decreased (F = 3.67; p = 0.044) until day 28 (45.9 +/- 13.1). CD68 immunostaining also significantly decreased (F = 6.75; p = 0.007) from day 7 (49.5 +/- 10.4) to day 28 (19.5 +/- 8.9). A positive and statistically significant correlation was observed between the evolutions of these two variables. The material had been almost completely resorbed at day 28. Among cells present at the granuloma, anti-MMP-2 immunostaining was predominant and more intense in macrophages, yet lightly immunolabeled multinucleated giant cells were found in close contact with the material. Thus, considering the experimental limitations of this study, we concluded that MMP-2 produced by macrophages participates in the resorption of demineralized bovine bone.

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Anorganic Bovine Bone Supports Osteoblastic Cell Attachment and Proliferation

Abstract: These results demonstrate that this anorganic bovine bone graft material is able to support the attachment and proliferation of osteoblastic cells.

Cited by 60 publications

References 18 publications

Three-dimensional cultivation of human osteoblast-like cells on highly porous natural bone mineral

Three-dimensional cultivation of human osteoblast-like cells on highly porous natural bone mineral

Inorganic polyphosphate adsorbed onto hydroxyapatite for guided bone regeneration: An animal study

Expression of Metalloproteinase 2 in the Cell Response to Porous Demineralized Bovine Bone Matrix

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