ANRIL overexpression globally induces expression and alternative splicing of genes involved in inflammation in HUVECs

Alimu, Wufuer; Luohemanjiang, Xiemusiye; Du, Lei; Lei, Jing; Shabier, Mayila; Han, Deng Feng; Ma, Junwu

doi:10.3892/mmr.2022.12915

Cited by 9 publications

(5 citation statements)

References 48 publications

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“…[30][31][32][33] The study revealed that ANRIL exerted an influence on the transcription levels of genes associated with the inflammatory response, NF-κB signaling pathway, type I interferon-mediated signal transduction pathway, and innate immune response in HUVEC. 34 Consistent with the literature, this research found that LPS caused increases in IL-1β, IL-6, and TNF-α expressions, and si-ANRIL could alleviate this phenomenon, which meant the alleviation of inflammation. Moreover, the knockdown of lnc-ANRIL released the oxidative stress induced by LPS.…”

Section: Discussionsupporting

confidence: 88%

Lnc‐ANRIL modulates the immune response associated with NF‐κB pathway in LPS‐stimulated bovine mammary epithelial cells

Lu,

Gu,

et al. 2023

Immunity Inflam & Disease

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BackgroundThe antisense noncoding RNA in the INK4 locus (ANRIL) has been confirmed related to multiple disease progression, but the role and exact mechanisms of lnc‐ANRIL in lipopolysaccharide (LPS)‐induced inflammation of bovine mammary epithelial cells (MAC‐T) remain unclear.AimsThis manuscript focused on expounding the functional role of lnc‐ANRIL through experiments performed in MAC‐T.MethodsAt the in vitro level, we established a Bovine mammary epithelial cell (BMEC) cell model of mastitis by LPS treatment. Transfection of siRNA was examined by immunofluorescence localization and RT‐qPCR. CCK8, clonogenic assay and EdU were used to detect the proliferation ability of the cells. Cell cycle and apoptosis were detected by flow cytometry and Western blot. The levels of inflammatory factors and oxidative stress markers were detected by ELISA kits.ResultsCell Counting Kit‐8, colony formation, and 5‐ethynyl‐20‐deoxyuridine were adopted and the data illustrated that LPS could significantly suppress the cell proliferation, while knockdown of lnc‐ANRIL expression obviously promoted MAC‐T cell proliferation compared with LPS or LPS + si‐NC group. Flow cytometry analysis demonstrated that lnc‐ANRIL could induce MAC‐T cell apoptosis. In addition, downregulation of lnc‐ANRIL affected LPS‐induced immune response by regulating inflammatory factor expressions and modulating the nuclear factor kappa B (NF‐κB) axis in MAC‐T cells.ConclusionOur results suggest that lnc‐ANRIL is involved in the regulation of cell proliferation, cell cycle, and cell apoptosis of MAC‐T cells, and plays an important role in the inflammatory and immune response of MAC‐T cells through the regulation of the NF‐κB pathway, proposing new therapeutic strategies for the treatment of innate immune response‐related disease such as bovine mastitis.

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Section: Discussionsupporting

confidence: 88%

Lnc‐ANRIL modulates the immune response associated with NF‐κB pathway in LPS‐stimulated bovine mammary epithelial cells

Lu,

Gu,

et al. 2023

Immunity Inflam & Disease

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“…ANRIL overexpression is associated with in ammatory response in IS [29], The possible mechanism involved in pro-in ammatory effect of ANRIL include; increase in NF-κB expression [30], type I interferon-mediated signal transduction pathway and innate immune response [31]. On the other hand, the ANRIL downregulation with anti-in ammatory effects has been suggested in in ammatory bowel disease (IBD) [32], attenuating the atherosclerosis progression in CAD patients[8] ,and acute ischemic stroke [33].…”

Section: Discussionmentioning

confidence: 99%

Diagnostic potential of LncRNAs-ANRIL and MIAT in the blood of patients with cerebral venous thrombosis

Zayani,

Hooshmandi,

Borhani-Haghighi

et al. 2023

Preprint

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Background: Long non-coding RNAs (LncRNAs) have been implicated as possible circulating stroke indicators. This study focused on the expression status of ANRILand MIAT in cerebral venous thrombosis (CVT) patients. Methods: In this study, fifty CVT patients and one hundred age/gender-matched individuals as controls were included. The circulating levels of ANRIL and MIATin the first 24 hours after admission were evaluated using the quantitative real-time polymerase chain reaction (RT-PCR) method. We compared the expression levels of ANRIL and MIAT between patients and controls using the independent two-sample t-test. Subgroup analysis was used toinvestigate the association of lncRNAs with clinical characteristics in CVT patients. ROC curve analyses conducted the diagnostic value of two lncRNAs in patient assessment. Results: The relative expression of lncRNAs ANRILand MIAT significantly decreased in patients compared to the control. ANRILand MIAT showed potential markers for discriminating CVT patients from the healthy controls with an AUC of 0.98 and 0.99, respectively. Conclusion: For the first time we found downregulation and diagnostic potential of LncRNAs-ANRILand MIAT in the blood of patients with CVT.

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“…Furthermore, ANRIL negatively and positively influences gene expression at the chromatin level [247]. ANRIL functions as a scaffold for PRC2 and, therefore, participates in epigenetic gene silencing [250] and is involved in alternative splicing in HEK293 and HUVEC cells [251]. Through these mechanisms, ANRIL contributes to tumourigenesis processes, increasing cell proliferation, migration, invasion, and metastasis and suppressing apoptosis and senescence [248].…”

Section: Emerging Lncrna Biomarkers Of Gbmmentioning

confidence: 99%

lncRNA Biomarkers of Glioblastoma Multiforme

Pokorná,

Černá,

Boussios

et al. 2024

Biomedicines

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Long noncoding RNAs (lncRNAs) are RNA molecules of 200 nucleotides or more in length that are not translated into proteins. Their expression is tissue-specific, with the vast majority involved in the regulation of cellular processes and functions. Many human diseases, including cancer, have been shown to be associated with deregulated lncRNAs, rendering them potential therapeutic targets and biomarkers for differential diagnosis. The expression of lncRNAs in the nervous system varies in different cell types, implicated in mechanisms of neurons and glia, with effects on the development and functioning of the brain. Reports have also shown a link between changes in lncRNA molecules and the etiopathogenesis of brain neoplasia, including glioblastoma multiforme (GBM). GBM is an aggressive variant of brain cancer with an unfavourable prognosis and a median survival of 14–16 months. It is considered a brain-specific disease with the highly invasive malignant cells spreading throughout the neural tissue, impeding the complete resection, and leading to post-surgery recurrences, which are the prime cause of mortality. The early diagnosis of GBM could improve the treatment and extend survival, with the lncRNA profiling of biological fluids promising the detection of neoplastic changes at their initial stages and more effective therapeutic interventions. This review presents a systematic overview of GBM-associated deregulation of lncRNAs with a focus on lncRNA fingerprints in patients’ blood.

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ANRIL overexpression globally induces expression and alternative splicing of genes involved in inflammation in HUVECs

Cited by 9 publications

References 48 publications

Lnc‐ANRIL modulates the immune response associated with NF‐κB pathway in LPS‐stimulated bovine mammary epithelial cells

Lnc‐ANRIL modulates the immune response associated with NF‐κB pathway in LPS‐stimulated bovine mammary epithelial cells

Diagnostic potential of LncRNAs-ANRIL and MIAT in the blood of patients with cerebral venous thrombosis

lncRNA Biomarkers of Glioblastoma Multiforme

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