ant(6)-I Genes Encoding Aminoglycoside O-Nucleotidyltransferases Are Widely Spread Among Streptomycin Resistant Strains of Campylobacter jejuni and Campylobacter coli

Hormeño, Lorena; Ugarte‐Ruiz, María; Palomo, Gonzalo; Borge, Carmen; Florez‐Cuadrado, Diego; Vadillo, S.; Píriz, Segundo; Domı́nguez, Lucas; Campos, Maria Jorge; Quesada, Alberto

doi:10.3389/fmicb.2018.02515

Cited by 18 publications

(20 citation statements)

References 35 publications

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“…On another hand, even though our study indicates a low occurrence of determinants associated with aminoglycoside resistance in the Campylobacter genomes tested, these determinants displayed a great diversity, since 9 different aminoglycoside resistance genes, as well as point mutations in rpsL , were found ( Figure 1 ). These findings are in agreement with those reported previously ( Qin et al, 2012 ; Zhao et al, 2015 , 2016 ; Cantero et al, 2018 ; Fabre et al, 2018 ; Hormeno et al, 2018 ; Whitehouse et al, 2018 ).…”

Section: Discussionsupporting

confidence: 94%

Genomic Analysis Reveals the Genetic Determinants Associated With Antibiotic Resistance in the Zoonotic Pathogen Campylobacter spp. Distributed Globally

Rivera-Mendoza¹,

Martínez-Flores²,

Santamaría³

et al. 2020

Front. Microbiol.

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The genus Campylobacter groups 32 Gram-negative bacteria species, several being zoonotic pathogens and a major cause of human gastroenteritis worldwide. Antibiotic resistant Campylobacter is considered by the World Health Organization as a high priority pathogen for research and development of new antibiotics. Genetic elements related to antibiotic resistance in the classical C. coli and C. jejuni species, which infect humans and livestock, have been analyzed in numerous studies, mainly focused on local geographical areas. However, the presence of these resistance determinants in other Campylobacter species, as well as in C. jejuni and C. coli strains distributed globally, remains poorly studied. In this work, we analyzed the occurrence and distribution of antibiotic resistance factors in 237 Campylobacter closed genomes available in NCBI, obtained from isolates collected worldwide, in different dates, from distinct hosts and comprising 22 Campylobacter species. Our data revealed 18 distinct genetic determinants, genes or point mutations in housekeeping genes, associated with resistance to antibiotics from aminoglycosides, β-lactams, fluoroquinolones, lincosamides, macrolides, phenicols or tetracyclines classes, which are differentially distributed among the Campylobacter species tested, on chromosomes or plasmids. Three resistance determinants, the bla OXA−493 and bla OXA−576 genes, putatively related to β-lactams resistance, as well as the lnu(AN2) gene, putatively related to lincosamides resistance, had not been reported in Campylobacter; thus, they represent novel determinants for antibiotic resistance in Campylobacter spp., which expands the insight on the Campylobacter resistome. Interestingly, we found that some of the genetic determinants associated with antibiotic resistance are Campylobacter species-specific; e.g., the bla OXA−493 gene and the T86V mutation in gyrA were found only in the C. lari group, whereas genes associated with aminoglycosides resistance were found only in C. jejuni and C. coli. Additional analyses revealed how are distributed the

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Section: Discussionsupporting

confidence: 94%

Genomic Analysis Reveals the Genetic Determinants Associated With Antibiotic Resistance in the Zoonotic Pathogen Campylobacter spp. Distributed Globally

Rivera-Mendoza¹,

Martínez-Flores²,

Santamaría³

et al. 2020

Front. Microbiol.

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“…, Table ). The genes ant‐like A and ant‐like B have been described before as separate genes (Olkkola et al ., ) and later revised as ant(6)‐Ie (Hormeño et al ., ). To avoid the issues of gene duplication and gene paralogues they are considered as separate genes in this study.…”

Section: Resultsmentioning

confidence: 97%

“…Antibiotics resistance to: C, ciprofloxacin; T, tetracycline; E, erythromycin; S, streptomycin; G, gentamicin. (Olkkola et al, 2016) and later revised as ant(6)-Ie (Hormeño et al, 2018). To avoid the issues of gene duplication and gene paralogues they are considered as separate genes in this study.…”

Section: Amr Isolates Are Distributed Across Highly Structured Populamentioning

confidence: 99%

Gene pool transmission of multidrug resistance among Campylobacter from livestock, sewage and human disease

Mourkas

Florez‐Cuadrado

Pascoe

et al. 2019

Environmental Microbiology

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Summary The use of antimicrobials in human and veterinary medicine has coincided with a rise in antimicrobial resistance (AMR) in the food‐borne pathogens Campylobacter jejuni and Campylobacter coli. Faecal contamination from the main reservoir hosts (livestock, especially poultry) is the principal route of human infection but little is known about the spread of AMR among source and sink populations. In particular, questions remain about how Campylobacter resistomes interact between species and hosts, and the potential role of sewage as a conduit for the spread of AMR. Here, we investigate the genomic variation associated with AMR in 168 C. jejuni and 92 C. coli strains isolated from humans, livestock and urban effluents in Spain. AMR was tested in vitro and isolate genomes were sequenced and screened for putative AMR genes and alleles. Genes associated with resistance to multiple drug classes were observed in both species and were commonly present in multidrug‐resistant genomic islands (GIs), often located on plasmids or mobile elements. In many cases, these loci had alleles that were shared among C. jejuni and C. coli consistent with horizontal transfer. Our results suggest that specific antibiotic resistance genes have spread among Campylobacter isolated from humans, animals and the environment.

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“…of human origin were isolated between 2010 and 2012 (139 strains; 132 C. jejuni and seven C. coli) and were described previously [21][22][23]. Cultivation procedures of the isolates included culture on blood agar in a microaerophilic atmosphere (CampyGen™; Thermo Scientific, Lenexa, KS, USA) at 42 • C for 24-48 h [21,22]. Tetracycline minimal inhibitory concentrations (MICs) of the isolates were determined by agar dilution methods according to the Clinical and Laboratory Standards Institute CLSI [24].…”

Section: Microbial Growth and Antibiotic Resistance Testingmentioning

confidence: 99%

Occurrence of tet(O/M/O) Mosaic Gene in Tetracycline-Resistant Campylobacter

et al. 2020

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Campylobacter is one of the most important microorganisms responsible for foodborne diseases in the EU. In this study, we investigated resistance to tetracycline in 139 Campylobacter jejuni and Campylobacter coli samples isolated from human clinical cases. From these, 110 were resistant to tetracycline, with MIC (minimal inhibitory concentration) varying in a range of 1 to >512 μg/mL, and 109 (78.4%) carried tet(O), a gene that confers resistance to tetracycline through the expression of a protein that confers protection to the ribosome. Amongst the tetracycline-resistant isolates, one C. jejuni (HCC30) was the only tet(O)-negative sample, presenting an MIC of 256 μg/mL. Instead, the mosaic gene tet(O/M/O) was found in HCC30 and, as far as we know, this is the first description of this chimeric gene originating from homologous recombination between tet(O) and tet(M). The previously described mosaic gene tet(O/32/O), also found in Campylobacter, presents a chimeric structure very similar to that of tet(O/M/O), affecting domains II and III of encoded proteins distantly related to the elongation factor G (EF-G). The tet(O/M/O) mosaic gene has been found in nucleotide databases in several genomes of Campylobacter isolated from different origins, indicating its frequent acquisition, even though it can be undetected through screening by PCR with specific tet(O) primers. In this work, we address the improvement of classical PCR to efficiently diagnose the most prevalent tetracycline resistance determinants in Campylobacter, including tet(O/M/O), which should be taken into account in the optimization of campylobacteriosis treatments.

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ant(6)-I Genes Encoding Aminoglycoside O-Nucleotidyltransferases Are Widely Spread Among Streptomycin Resistant Strains of Campylobacter jejuni and Campylobacter coli

Cited by 18 publications

References 35 publications

Genomic Analysis Reveals the Genetic Determinants Associated With Antibiotic Resistance in the Zoonotic Pathogen Campylobacter spp. Distributed Globally

Genomic Analysis Reveals the Genetic Determinants Associated With Antibiotic Resistance in the Zoonotic Pathogen Campylobacter spp. Distributed Globally

Gene pool transmission of multidrug resistance among Campylobacter from livestock, sewage and human disease

Occurrence of tet(O/M/O) Mosaic Gene in Tetracycline-Resistant Campylobacter

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