Antagonism of Selected Bacterial Strains to<i>Stemphylium vesicarium</i>and Biological Control of Brown Spot of Pear Under Controlled Environment Conditions

Seguí, Emilio Montesinos; Bonterra, A.; Ophir, Yakir; Beer, Steven V.

doi:10.1094/phyto-86-856

Cited by 32 publications

(17 citation statements)

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“…The strains DTBS-5, METBS-31, METBE-35, UTBE-26 and UTBE-30 have potential for highest inhibition effect against bacterial pathogen. These results confirm the previous studies reporting the antagonizing activity of Bacillus species against R. solanacerarum (Li et al, 2008; Almoneafy et al, 2012; In this study, CPG medium was used for in vitro screening assay, because the medium was suitable for R. solanacearum growth and agrees with other earlier studies, which reported that the type of culture medium strongly affects antagonistic activity mediating the production of chemical substances responsible for inhibition (Montesinos et al, 1996). Bacillus species are known as very efficient producers of antibiotic molecules of three main family, surfactins, iturins and fengycins.…”

Section: Discussionsupporting

confidence: 92%

Genetic diversity of iturin producing strains of Bacillus species antagonistic to Ralstonia solanacerarum causing bacterial wilt disease in tomato

Singh¹,

Yadav²,

Sinha³

et al. 2013

Afr. J. Microbiol. Res.

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Bacillus spp. is a potential bacterial antagonist to manage bacterial wilt disease of tomato incited by Ralstonia solanacearum, which is one of the most threatening diseases of tomato in India. Genetic diversity of Bacillus strains and their potentiality to control bacterial wilt of tomato isolated from rhizospheric soil and endophytic tomato plants from different agro-climatic regions of India were studied. Rhizospheric soil and plants of tomato were pasteurized at 80°C for 15 min before dilution and then inoculated onto the Petri plates containing tryptic soy agar medium and incubated at 28± 2°C. Out of 250 isolates of Bacillus species, 47 strains showed antagonistic ability against R. solanacearum. Maximum growth of R. solanacearum was inhibited by strain DTBS-5 to form inhibition zone of 5.5 cm 2 in vitro and lowest wilt incidence of 14.3 and 7.6 % in Pusa Ruby and Arka Abha cultivars under glass house conditions, respectively. Plants treated with strain JTBS-9 had maximum fresh weight of 42.0 and 49.0 g and dry weight 6.1 and 6.6 g in tomato cultivars Pusa Ruby and Arka Abha after 45 days of transplanting, respectively. Out of 47 strains, 11 strains of Bacillus spp. were detected as iturin antibiotic producing strains by using iturin gene based marker. Genetic variability was found in Bacillus spp., which was made 5 clusters at 50% similarity coefficient. However, iturin producing and iturin nonproducing strains as well as rhizospheric and endophytic Bacillus spp. could not be distinguished by using 16S rRNA sequence analysis and genetic fingerprinting.

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Section: Discussionsupporting

confidence: 92%

Genetic diversity of iturin producing strains of Bacillus species antagonistic to Ralstonia solanacerarum causing bacterial wilt disease in tomato

Singh¹,

Yadav²,

Sinha³

et al. 2013

Afr. J. Microbiol. Res.

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“…Strains of Pseudomonas fluorescens and Pantoea agglomerans antagonistic to S. vesicarium were found to be effective in preventing brown spot infections when sprayed on potted pear plants in the greenhouse (Montesinos et al 1996). However, the same strains were ineffective when tested as spray Table 5 A global analysis of the efficacy of different treatments aimed at controlling brown spot of pear by sanitation expressed as reduction of the disease incidence at harvest or of area under disease progress curve (AUDPC-incidence) compared to a non-treated control on fruit and leaves Treatment a Number of trials performed Incidence at harvest AUDPC-incidence Efficacy range (%) Efficacy range (%)…”

Section: Discussionmentioning

confidence: 99%

Control of brown spot of pear by reducing the overwintering inoculum through sanitation

Llorente

Vilardell

et al. 2010

Eur J Plant Pathol

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Stemphylium vesicarium, the causal agent of brown spot of pear, overwinters in the leaf residues of pear and herbaceous plants of the orchard floor. Pseudothecia of the teleomorph, Pleospora allii, are formed on these residues where they produce ascospores. New methods were tested aimed at reducing this overwintering inoculum and increasing the efficacy of control of brown spot of pear. Sanitation methods were evaluated in nine trials in Girona (Spain) and Ferrara (Italy) over a 4-year period. The sanitation methods were leaf litter removal in December to February, and application of biological control agents (commercial formulates of Trichoderma spp.) to the orchard ground cover from February to May. Fungicides were also applied to the trees during the pear-growing season, scheduled according to the BSPcast model. The different methods were tested as stand-alone applications or in combination. All methods consistently reduced the disease incidence at harvest on fruit with an efficacy between 30 to 60% for leaf litter removal and more than 60% for the combination of leaf litter removal and biological control. Efficacy of sanitation alone (leaf litter removal and biological control) in reducing the brown spot level on fruit was similar in most of the trials to the efficacy obtained when fungicides were applied alone. However, integration of sanitation methods and fungicides did not improve the efficacy of disease control over the level provided by fungicides alone.

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“…Evaluation of bacteria in vitro as potential antagonists from a wide range of agricultural crops has been reported (1,5,11,23,27). This is, however, the first report in which bacteria from pistachio leaves, buds, and fruits have been isolated and their antagonistic potential evaluated against the panicle and shoot blight pathogen.…”

Section: Discussionmentioning

confidence: 99%

Antagonism of Paenibacillus lentimorbus to Botryosphaeria dothidea and Biological Control of Panicle and Shoot Blight of Pistachio

Chen¹,

Morgan

Felts

et al. 2003

Plant Disease

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A potential microbial fungicide, Paenibacillus lentimorbus isolate CBCA-2, against Botryosphaeria dothidea, the pistachio panicle and shoot blight fungus, was obtained from healthy pistachio leaves by both in vitro and in vivo screening techniques. CBCA-2 caused 100% inhibition of pycnidiospore germination after 24 h incubation at 25°C. Malformation of pycnidiospores and hyphae, and lysis and swollen pycnidiospores of B. dothidea occurred in the presence of cell suspensions of CBCA-2. Among the five media tested, nutrient yeast dextrose broth significantly increased the production of antifungal compounds. Application of culture filtrates of CBCA-2 suppressed disease on detached pistachio leaves, but washed bacterial cells did not inhibit lesion development. Development of lesions on excised dormant stems was inhibited only when the culture filtrate was applied before fungal inoculation. Survival of the CBCA-2 after treatment with azoxystrobin (Abound), benomyl (Benlate), tebuconazole (Elite), propiconazole (Break), or trifloxystrobin (Flint) at the highest recommended concentration was not affected, but survival was affected by iprodione (Rovral). Spraying a suspension of CBCA-2 on pruning wounds before inoculation with pycnidiospores of B. dothidea significantly reduced infection compared with the unsprayed, inoculated controls.

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Antagonism of Selected Bacterial Strains toStemphylium vesicariumand Biological Control of Brown Spot of Pear Under Controlled Environment Conditions

Cited by 32 publications

References 0 publications

Genetic diversity of iturin producing strains of Bacillus species antagonistic to Ralstonia solanacerarum causing bacterial wilt disease in tomato

Genetic diversity of iturin producing strains of Bacillus species antagonistic to Ralstonia solanacerarum causing bacterial wilt disease in tomato

Control of brown spot of pear by reducing the overwintering inoculum through sanitation

Antagonism of Paenibacillus lentimorbus to Botryosphaeria dothidea and Biological Control of Panicle and Shoot Blight of Pistachio

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