Antagonistic properties and biocompatibility as important principles for development of effective and biosafety probiotic drugs

Abstract

Antagonistic properties and peculiarities of inter-strain interactions of probiotic microorganisms were investigated. The study of the antagonistic activity of probiotic strains was carried out by the methods of perpendicular strokes (or delayed antagonism) and the diffusion method (block modification) in relation to opportunistic and pathogenic microorganisms. Most probiotic strains had moderate to high antagonistic activity against both gram-positive and gram-negative pathogens. The results obtained allowed us to select the most active strains, namely L. plantarum AS-41, L. acidophilus A-14, B. subtilis S-2 and B. subtilis S-18, which have a high probiotic potential. To assess biocompatibility, the method of co-cultivation of the selected strains on a modified agar MRS medium was used. The strains that showed biocompatibility of the type “mutual neutrality” or “contact progression” were selected to create a consortium of probiotic microorganisms. Further study of the investigated strains of probiotic bacteria opens up the possibilities of their use for the manufacture of a combined preparation for veterinary purposes, for the prevention and treatment of diseases of the gastrointestinal tract of farm animals.

Microorganism Isolates Stimulate Forest Crop Growth and Protection

“…A determination of antifungal properties of the biologically active metabolites of isolated microorganisms in relation to pathogens of various diseases of agricultural plants was carried out by the method described in [25][26][27][28][29].…”

Antagonistic Activity of Lactobacilli Probiotic Strains in Co-Cultivation

“…1. В аналогічних дослідженнях інших авторів показано як механізми, що засновані на харчових взаємодіях, можуть бути використані для створення ефективних комбінацій молочнокислих бактерій [6,10] і доведено можливість спільного культивування окремих штамів молочнокислих бактерій у вдало підібраних парах, а також отримання багатокомпонентних пробіотичних препаратів [15].…”

Parameters of acute toxicity and sensitizing properties of a microbiological preparation based on L. acidophilus IV138 and B. subtilis GA24 strains

“…In conditions of intensive industrial animal husbandry in limited area industrial premises with a high density of livestock, the use of feed containing antibiotics in diets has led to the emergence of opportunistic and pathogenic microorganism's resistant to the means of this group [1][2][3]. In order to obtain high-quality products, primarily from the point of view of their environmental safety, from January 1, 2006, the use of growth stimulants based on antibiotics in raising farm animals was banned in the animal husbandry of the European Union.…”

“…It has been shown that the normal intestinal microbiota performs a number of significant functions, among which, it should be noted, providing colonization resistance, bactericidal, bacteriostatic and antitoxic effects, maintaining the optimal level of metabolism and enzymatic processes, and immune status [2,[4][5][6][7][8][9]. The importance of intestinal microecology in ensuring the health of the organism of farm animals as a whole is not in doubt, since minimal violations of its composition lead to the appearance and development of various infectious diseases.…”

“…The importance of intestinal microecology in ensuring the health of the organism of farm animals as a whole is not in doubt, since minimal violations of its composition lead to the appearance and development of various infectious diseases. Currently, for the correction of microecological disorders of the intestines of young farm animals caused by the action of pathogenic factors, substitution therapy aimed at the regular introduction of live probiotic microorganisms has acquired particular importance [1,2,[10][11][12]. According to the definition, which was formulated by members of the FAO and WHO expert group, "probiotics" are "live active strains of various microorganisms that benefit the health of the host when used in appropriate quantities" [13].…”