Anterior cruciate ligament constructs fabricated from human mesenchymal stem cells in a collagen type I hydrogel

Nöth, Ulrich; Schupp, K.; Heymer, A; Kall, S.; Jakob, Franz; Schütze, Norbert; Baumann, Bernd; Barthel, Thomas; Eulert, J.; Hendrich, Christian

doi:10.1080/14653240500319093

Cited by 117 publications

(87 citation statements)

References 22 publications

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“…109 Similarly, human mesenchymal stem cells embedded in a collagen gel form a wavy orientated tissue containing ligament-like ECM when subjected to cyclic stretching. 110 Stem cells can distinguish between different types of mechanical stretching. Uniaxial stretching transiently enhances the expression of smooth muscle markers like smooth muscle a-actin in human BMSCs, whereas equilateral stretching inhibits smooth muscle differentiation.…”

Section: Mechanical Forcesmentioning

confidence: 99%

Review:Ex VivoEngineering of Living Tissues with Adult Stem Cells

et al. 2006

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Adult stem cells have the potential to revolutionize regenerative medicine with their unique abilities to self-renew and differentiate into various phenotypes. This review examines progress and challenges in ex vivo tissue engineering with adult stem cells. These rare cells are harvested from a variety of tissues, including bone marrow, adipose, skeletal muscle, and placenta, and differentiate into cells of their own lineage and in some cases atypical lineages. Insight into the stem cell niche leads to the identification of matrix components, soluble factors, and physiological conditions that enhance the ex vivo amplification and differentiation of stem cells. Scaffolds composed of metals, naturally occurring materials, and synthetic polymers organize stem cells into complex spatial groupings that mimic native tissue. Cell signals from covalently bound ligands and slowly released regulatory factors in scaffolds direct stem cell fate. Future advances in stem cell biology and scaffold design will ultimately improve the efficacy of tissue substitutes as implants, in research, and as extracorporeal devices.

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Section: Mechanical Forcesmentioning

confidence: 99%

Review:Ex VivoEngineering of Living Tissues with Adult Stem Cells

et al. 2006

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“…Different synthetic and natural polymers were used for this purpose, including polyethylene glycol (PEG), and copolymers containing PEG [486,510], hyaluronic acid (HA) [511] after an oxidation reaction through HA-tyramine conjugates [505] and as a result of the formation between HA-SH [492,512] and Michael addition [491,513], collagen and gelatin hydrogels mostly cross-linked using glutaraldehyde, genipin or water-soluble carbodiimides [513][514][515], chitosan [516][517][518][519], dextran 192 [520,521] and alginate [522]. Hydrogels were used for reconstruction of the retina [523], ligament [524], fatty tissue [465], kidneys [525], muscles [526], blood vessels [527,528], and also heart, neural cells, invertebral discs, bones and gristle [459]. Hydrogels were used to prevent adhesions [529,530,531], to promote cellular adhesion [490,532,533].…”

Section: Selection Of Technologies Of Implantable Devices In Regeneramentioning

confidence: 99%

Introductory Chapter: Multi-Aspect Bibliographic Analysis of the Synergy of Technical, Biological and Medical Sciences Concerning Materials and Technologies Used for Medical and Dental Implantable Devices

Dobrzański¹

2018

Biomaterials in Regenerative Medicine

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“…However, differentiation of human MSC (hMSCs) into fibroblasts has been assumed to be the default pathway, yet with little experimental evidences. Altman et al [2] and Noth et al [3] showed that hMSCs are differentiated into ligament fibroblasts by cyclic mechanical stimulation. Also, it was reported that various growth factors and their combinations could promote the differentiation of hMSCs into fibroblasts [4], [5].…”

Section: Introductionmentioning

confidence: 99%

Fibroblastic Differentiation of Human Mesenchymal Stem Cells using Connective Tissue Growth Factor

Lee¹,

Moioli²,

Mao³

2006

2006 International Conference of the IEEE Engineering in Medicine and Biology Society

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The present study was designed to explore an ex vivo culturing protocol for fibroblastic differentiation of human mesenchymal stem cells (hMSCs) using connective tissue growth factor (CTGF). Fibroblastic differentiation from stem cells is of widespread significance in the engineering of virtually all tissues including tendons, ligaments, periodontal ligament, cranial sutures and as interstitial filler of all organs. The treatment with 100 ng/ml of recombinant human CTGF and 50 μg/ml ascorbic acids on monolayer cultured hMSCs showed significant increases in type I collagen and tenascin-C (Tn-C) contents by 2 and 4 wks. In addition, CTGF-treated hMSCs failed to show osteogenic or chondrogenic differentiation. The present data show that CTGF is an effective induction factor for fibroblastic differentiation of hMSCs. These findings have implications for engineering fibrous tissue by providing the initial evidence of a reproducible protocol for fibroblastic differentiation of hMSCs.

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Anterior cruciate ligament constructs fabricated from human mesenchymal stem cells in a collagen type I hydrogel

Cited by 117 publications

References 22 publications

Review:Ex VivoEngineering of Living Tissues with Adult Stem Cells

Review:Ex VivoEngineering of Living Tissues with Adult Stem Cells

Introductory Chapter: Multi-Aspect Bibliographic Analysis of the Synergy of Technical, Biological and Medical Sciences Concerning Materials and Technologies Used for Medical and Dental Implantable Devices

Fibroblastic Differentiation of Human Mesenchymal Stem Cells using Connective Tissue Growth Factor

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