Anthelminthic activity of Asta Churna

Devi, K H Bijubala; Indumathy, Sivanjah; Rathinambal, V; Uma, K.; Kavimani, S; Balu, V.

doi:10.4314/ijhr.v2i1.55399

Cited by 10 publications

(8 citation statements)

References 2 publications

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“…The use of chemotherapeutic drugs to control internal and external parasites is a widespread practice in livestock production and several broad-spectrum anthelmintics are available in the market for the control of helminthosis. Currently, failure of anthelmintics and reduced efficacy due to resistance of nematodes in sheep and goats are becoming a threat in some countries and are of increasing importance in certain African countries [ 2 , 3 ]. According to Waller [ 4 ], most of the nematodes of domestic animals have shown resistance to common anthelmintics especially in warm and humid parts of the world and this has been suspected to be due to frequent dosing and poor therapeutic strategies [ 5 – 8 ].…”

Section: Introductionmentioning

confidence: 99%

Effectiveness Evaluation of Levamisole, Albendazole, Ivermectin, andVernonia amygdalinain West African Dwarf Goats

Adediran

Uwalaka

2015

Journal of Parasitology Research

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Anthelmintic drug resistance has led to the search for alternatives in controlling helminth infections. Fifty West African Dwarf goats without history of anthelmintic treatment were divided equally into five groups. Group A was treated with ivermectin injection subcutaneously, group B with levamisole subcutaneously, group C with albendazole orally, and group D with aqueous extract of Vernonia amygdalina and group E was untreated control. Faecal samples were collected before treatment from each animal and larval culture was carried out. Faecal egg count reduction (FECR) test was carried out for each group and the data analysed using FECR version 4 to calculate percent reduction in faecal egg count. Predominant helminth infections from larval culture were Haemonchus contortus (70%), Trichostrongylus spp. (61%), and Oesophagostomum spp. (56%). Mixed infection was present in all the animals. From the FECR test Vernonia amygdalina extract was more effective against helminths (100%), compared to ivermectin 96%, levamisole 96%, and albendazole 99%. The lower 95% confidence limit was 89 for ivermectin and levamisole and 91 for albendazole. There is low resistance to ivermectin and levamisole and susceptibility to albendazole while V. amygdalina has great potentials that could be explored for the treatment of helminth diseases in goats.

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Section: Introductionmentioning

confidence: 99%

Effectiveness Evaluation of Levamisole, Albendazole, Ivermectin, andVernonia amygdalinain West African Dwarf Goats

Adediran

Uwalaka

2015

Journal of Parasitology Research

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“…The time of paralysis of individual worms was noted. The paralyzed worms that not recover in normal saline were considered as dead [26,27].…”

Section: Petri Dish Methodsmentioning

confidence: 99%

“…The anthelmintic activity was evaluated in Indian adult earthworm (Pheretima posthuma) due to its anatomical and physiological resemblance with the intestinal roundworm parasites of human beings [21,25] by Devi et al method [26]. Piperazine citrate served as a standard drug (10 mg/ml), dimethylformamide used as a co-solvent for the extract, saline as a solvent blank, student's organ bath (it's consist of an outer jacket, made up of Perspex; the inner organ bath made up of glass with a capacity 40 ml, length 40 cm and width 6 cm; thermostatically controlled heating rod; stirrer, glass coil, and tissue holder) Sherrington rotating drum and Frontal writing lever were used.…”

Section: In Vitro Anthelmintic Assaymentioning

confidence: 99%

Lead Molecule Identification From Vitex Trifolia Linn for Helminthiasis Using in Vitro and in Silico Methods

Muthukrishnan

Ragunath²,

Varghese³

2020

Int J Pharm Pharm Sci

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Objective: The study was an attempt to discover a lead molecule to treat helminthiasis using Vitex trifolia. Linn (V. folia Linn) through sterile effect, in vitro and in silico evaluation. Methods: The antibacterial activity was done by Kirby-Bauer disc diffusion method in three different concentrations of extract and in vitro anthelmintic activity was carried out by petri dish and organ bath method. Further, the in silico docking studies were carried out by 11 phytoconstituents against phosphoethanolamine methyltransferase (4FGZ) using Auto Dock 4.2, it was working based on the principle of Lamarckian genetic algorithm. In docking studies, three important parameters such as binding energy, inhibition constant and intermolecular energy are determined. Results: The extracts showed an antibacterial effect in three different concentrations. At 16 mcg/disc a significant effect was observed when compared to blank and ciprofloxacin 5 mcg/disc. The anthelmintic activity in the petri dish method, means paralyzing time of Pheretimaposthuma with the dose of 25, 50 and 100 mg/ml were 13.78, 5.79 and 4.57 min respectively and Piperazine citrate (10 mg/ml) showed paralysis in 21.58 min. In the organ bath method, the time for paralysis of the worm was recorded on a slow-moving Sherrington rotating drum and the study report showed that paralyzing time was decreased at increasing concentrations of the extract. The results of in silico studies exhibited a binding energy of-10.25kcal/mol, inhibitory constant (Ki) 30.91nM, intermolecular energy,-10.84kcal/mol for abietatriene-3-ol which is lesser than the standard ligand phosphoethanolamine (-6.03kcal/mol, 38.29µM,-7.82kcal/mol) respectively. Conclusion: The study reports conclude that the active constituents in V. folia Linn having better anthelmintic activity, thus the active constituents may be optimized and make way to a new moiety for the treatment of helminthiasis.

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“…Parasitic worms are also challenging pathogens for human beings, they pose a major threat to public health and contribute to prevalence of malnutrition, anaemia, eosinophilia, and pneumonia (Kulkarni et al, 2010). Unfortunately, currently being used anthelmintic drugs have adverse effects and their indiscriminate use is leading to resistance of parasites (Devi et al, 2009), therefore, there is a foremost problem in treatment of helminths diseases. Hence there is an increasing demand towards natural anthelmintics.…”

Section: Introductionmentioning

confidence: 99%

Phytochemical Screening of Leaves from Mangifera indica and Morus alba for Bioactive Compounds

Muhammadi¹,

Shafiq

Hussain

2020

J. Plant Environ.

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The aqueous, methanol and DMSO extracts from leaves of Mangifera indica and Morus alba were screened for bioactive compounds. Antimicrobial activity and MIC (mg/ml) were determined using agar-well diffusion and serial dilution methods respectively against human pathogenic bacteria and fungi. Antioxidant activities (%) were determined against DPPH free radicals, superoxide anion and Fe+2 whereas antiurease activity (%) was by indophenol method. The time taken to set paralysis and death of Pheritima posthuma was observed after released into extract. Phytochemical analysis detected carbohydrates, alkaloids, phenols, flavonoids, terpenoids, saponins, tannins, steroids, resins, anthraquinones, cardiac glycosides and phlobatannins. All extracts more or less showed the activity against Gram-positive and -negative bacteria producing zone of inhibition 15±0.57 to 43.66±0.66 mm and MIC values 13±1.64 to 100±0.35 mg/ml. Similarly, for fungi, zone of inhibition and MIC values were 9±0.57 to 34±0.57 mm and 23±1.25 115±0.55 g/ml respectively. Activities against DPPH, superoxide anion and Fe+2 free radicals were found 13.68±0.74 to 93.70±0.76 %, 50.23±0.43 to 95.7±0.21 % and 52.76±0.23 to 98.82±0.42% respectively, of these, the maximum activities surpassed the n-propyl gallate (91.31 %) and EDTA (98.05 %). Similarly, urease was also inhibited (33 to 95.23%) by all extracts. All extracts were also caused paralysis followed by subsequent death (13.5/26.87 to 39/60.06 min) of P. posthuma, of these methanolic extract from M. indica was least than that by albendazole (30.45/52 min) and piperazine citrate (14.35/36 min). The results provide scientific basis for traditional use of these extracts as affordable strategies for treatments of infectious, inflammatory and degenerative diseases, caused by bacteria, fungi, free radicals, urease and helminths.

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Anthelminthic activity of Asta Churna

Cited by 10 publications

References 2 publications

Effectiveness Evaluation of Levamisole, Albendazole, Ivermectin, andVernonia amygdalinain West African Dwarf Goats

Effectiveness Evaluation of Levamisole, Albendazole, Ivermectin, andVernonia amygdalinain West African Dwarf Goats

Lead Molecule Identification From Vitex Trifolia Linn for Helminthiasis Using in Vitro and in Silico Methods

Phytochemical Screening of Leaves from Mangifera indica and Morus alba for Bioactive Compounds

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