Anthelmintic effect of plant extracts containing condensed and hydrolyzable tannins on Caenorhabditis elegans, and their antioxidant capacity

Katiki, Luciana Morita; Ferreira, Jorge; Gonzalez, Javier M.; Zajac, Anne M.; Lindsay, David S.; Chagas, A. C. de S.; Amarante, Alessandro Francisco Talamini do

doi:10.1016/j.vetpar.2012.09.030

Cited by 77 publications

(54 citation statements)

References 57 publications

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“…The total antioxidant capacity is the sum of the lipophilic (ORACLipo) and hydrophilic (ORACHydro) fractions extracted from plants by hexane (lipophilic) and 70:30 acetone:aqueous buffer (hydrophilic). Our ORAC data ( Table 4) confirmed those of others [63,64] who reported that the hydrophilic fractions of plant extracts contain most (68%-99%) of the total antioxidant capacity of shoots. Alfalfa shoots grown with saline water had 94%-96% of the total antioxidant capacity in the hydrophilic fraction with only 4%-6% in the lipophilic fraction, indicating that alfalfa shoots are low in lipophilic antioxidants such as tocopherols, carotenes, and fatty acids.…”

Section: Antioxidant Capacity Of Alfalfasupporting

confidence: 90%

“…Phenolic compounds (including flavonoids) protect plants against the damaging effects of excessive reactive oxygen species (ROS) triggered by abiotic stresses, including salinity [60,61]. Although oxygen radical absorbance capacity (ORAC) has been widely accepted by industry to gauge the total antioxidant capacity of fruits, vegetables, spices, and other items consumed by humans, ORAC has only recently been used to estimate the antioxidant capacity of plants destined for livestock consumption [62][63][64]. The total antioxidant capacity is the sum of the lipophilic (ORACLipo) and hydrophilic (ORACHydro) fractions extracted from plants by hexane (lipophilic) and 70:30 acetone:aqueous buffer (hydrophilic).…”

Section: Antioxidant Capacity Of Alfalfamentioning

confidence: 99%

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Nutrient Composition, Forage Parameters, and Antioxidant Capacity of Alfalfa (Medicago sativa, L.) in Response to Saline Irrigation Water

et al. 2015

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Although alfalfa is moderately tolerant of salinity, the effects of salinity on nutrient composition and forage parameters are poorly understood. In addition, there are no data on the effect of salinity on the antioxidant capacity of alfalfa. We evaluated four non-dormant, salinity-tolerant commercial cultivars, irrigated with saline water with electrical conductivities of 3.1, 7.2, 12.7, 18.4, 24.0, and 30.0 dS·m −1 , designed to simulate drainage waters from the California Central Valley. Alfalfa shoots were evaluated for nutrient composition, forage parameters, and antioxidant capacity. Salinity significantly increased shoot N, P, Mg, and S, but decreased Ca and K. Alfalfa micronutrients were also affected by salinity, but to a lesser extent. Na and Cl increased significantly with increasing salinity. Salinity slightly improved forage parameters by significantly increasing crude protein, the net energy of lactation, and the relative feed value. All cultivars maintained their antioxidant capacity regardless of salinity level. The results indicate that alfalfa can tolerate moderate to high salinity while maintaining nutrient composition, antioxidant capacity, and slightly improved forage parameters, thus meeting the standards required for dairy cattle feed. OPEN ACCESSAgriculture 2015, 5 578

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Section: Antioxidant Capacity Of Alfalfasupporting

confidence: 90%

Section: Antioxidant Capacity Of Alfalfamentioning

confidence: 99%

Nutrient Composition, Forage Parameters, and Antioxidant Capacity of Alfalfa (Medicago sativa, L.) in Response to Saline Irrigation Water

et al. 2015

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“…Throughout the last ten years, several studies have been carried out with plants rich in condensed tannins (CT), their main focus being the use of such in the control of GIN in small ruminants (MARLEY et al, 2003;MINHO et al, 2008;KATIKI et al, 2013). …”

Section: Introductionmentioning

confidence: 99%

Ultrastructural changes in the Haemonchus contortus cuticle exposed to Acacia mearnsii extract

et al. 2015

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The parasite Haemonchus contortus is one of the most pathogenic for small ruminants in tropical and subtropical regions worldwide, including Brazil. The objective of this study was to evaluate the structural changes induced in adult H. contortus after in vitro contact with Acacia mearnsii extract (AE), using scanning electron microscopy. Adult nematodes were collected from a naturally infected lamb. In the in vitro assay the parasites were placed in contact with AE (100 mg ml -1 ), for two hours at 37 o C. The nematodes used in the assays (exposed to AE and the negative controls) were analyzed using an electron scanning microscope (quadruplicate per treatment). In all replicates, similar morphological alterations were observed on the entire extension of the cuticle of the specimens that remained in contact with the EA in vitro assays, none significant lesion was observed in the negative control (not exposed to AE). These results indicate the direct action of EA on the cuticle of H. contortus in in vitro trials. Key words: Gastrointestinal nematodes, scanning electron microscopy, structural changes, tannins ResumoHaemonchus contortus é um dos parasitos mais patogênicos para pequenos ruminantes em regiões tropicais e subtropicais em todo o mundo, inclusive no Brasil. O objetivo do trabalho foi analisar alterações ultraestruturais na cutícula de H. contortus após contato com extrato de Acacia mearnsii. Os parasitos expostos ao extrato de Acacia mearnsii (EA) e os controles negativos foram analisados ao microscópio eletrônico de varredura. No teste in vitro, adultos de H. contortus foram coletados de um ovino naturalmente infectado, e os parasitos foram colocados em contato com o EA (100 mg mL -1 ), durante duas horas à 37 o C. Os nematódeos expostos ao EA apresentaram alterações morfológicas em toda a extensão da cutícula. As alterações morfológicas não foram observadas no controle negativo. Estes resultados indicam a ação direta do EA na cutícula de H. contortus.

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“…Con base en el contenido de polifenoles en estos extractos, también probaron el efecto de tres fenoles en cepas silvestres y resistentes a antihelmínticos comerciales de C. elegans, lo que les permitió ver que el ácido elágico era una opción potencial para el tratamiento de infecciones por nematodos, incluso en casos de resistencia a antihelmínticos comerciales (92) . Katiki et al (93) probaron diferentes extractos de plantas en C. elegans y encontraron que plantas ricas en taninos hidrolizables tienen mayor actividad antihelmíntica que plantas ricas en taninos condensados. C. elegans también ha permitido probar la mejor opción de solvente para preparar los extractos (94,95) .…”

Section: Elegans Como Modelo Para Estudios De Actividad Antihelmínunclassified

El nematodo Caenorhabditis elegans como modelo para evaluar el potencial antihelmíntico de extractos de plantas

Peña

Vazquez

Sánchez

et al. 2017

Rev. Mex. Cienc. Pecu.

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RESUMENLos nematodos parásitos afectan la productividad pecuaria, dando lugar a pérdidas económicas considerables. Sin embargo, son pocos los antihelmínticos disponibles en el mercado y la resistencia de los parásitos a los existentes va en incremento, por lo que su control es actualmente ineficiente. En este sentido, la búsqueda de nuevas estrategias para controlar la parasitosis es una necesidad actual. Una posibilidad para el control estriba en la investigación de metabolitos derivados de plantas, ya que el conocimiento sobre su uso tradicional es un referente de inicio. Sin embargo, la eficacia antihelmíntica, así como los mecanismos de acción de plantas con uso tradicional como antiparasitarios, poco se han investigado científicamente, y en la mayoría de los casos se desconocen los compuestos activos responsables del efecto anti-parasitario. Una de las limitantes del estudio experimental del potencial antihelmíntico es la dificultad de trabajar con modelos parásitos. En este sentido, el nematodo de vida libre C. elegans es un modelo útil para entender el mecanismo de acción de drogas antihelmínticas. Por un lado, este nematodo se puede mantener y manipular en laboratorio con facilidad; por otro, comparte características fisiológicas y homología genética con nematodos parásitos, lo que permite comparaciones válidas. En esta revisión se pretende mostrar el potencial de la búsqueda de compuestos activos derivados de plantas tradicionalmente utilizadas como antiparasitarios como punto de partida en el desarrollo de nuevos fármacos antihelmínticos, así como resaltar las ventajas que C. elegans proporciona para entender los mecanismos de acción de nuevos compuestos. PALABRAS CLAVE: Resistencia

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Anthelmintic effect of plant extracts containing condensed and hydrolyzable tannins on Caenorhabditis elegans, and their antioxidant capacity

Cited by 77 publications

References 57 publications

Nutrient Composition, Forage Parameters, and Antioxidant Capacity of Alfalfa (Medicago sativa, L.) in Response to Saline Irrigation Water

Nutrient Composition, Forage Parameters, and Antioxidant Capacity of Alfalfa (Medicago sativa, L.) in Response to Saline Irrigation Water

Ultrastructural changes in the Haemonchus contortus cuticle exposed to Acacia mearnsii extract

El nematodo Caenorhabditis elegans como modelo para evaluar el potencial antihelmíntico de extractos de plantas

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