Anther and microspore culture of Hordeum vulgare L. cv. Igri

Hoekstra, S.; Zijderveld, M.H. van; Louwerse, Jeanine; Heidekamp, F.; Mark, F. van der

doi:10.1016/0168-9452(92)90182-l

Cited by 130 publications

(80 citation statements)

References 28 publications

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“…The regenerated plants were completely fertile doubled haploids (Hoekstra et al, 1992;1993;Hu and Kasha, 1999). The high frequency of completely fertile plants indicates that chromosome doubling must occur very early, most likely at the time of induction (Kasha et al, 2001;Seguí-Simarro and Nuez, 2008).…”

Section: Discussionmentioning

confidence: 99%

Study of androgenesis and spontaneous chromosome doubling in barley ( Hordeum vulgare L.) genotypes using isolated microspore culture

Kahrizi

Mohammadi²

2009

Acta Agronomica Hungarica

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This research aimed to study the androgenesis and spontaneous chromosome doubling of five barley genotypes using an isolated in vitro microspore culture technique, involving a completely randomized design (CRD) with three replications. Statistical analysis of embryogenesis and cytogenetic results showed that genotype had a significant effect on haploid embryogenesis (P<0.01) and on spontaneous chromosome doubling (P<0.05). The genotype Igri was found to have the highest potential to produce haploid embryos (1577 embryos from 100 anthers), followed by the genotypes Boyer/Rojo, Afzal/Turkman/Kavir, Ashar/Hebo and Agrigashar/Matico with 369, 304, 278 and 150 embryos from 100 anthers, respectively. The highest percentage of spontaneous chromosome doubling (76%) was observed for the genotype which had the lowest embryogenesis (Agrigashar/Matico) and the lowest (65%) for the genotype with the highest androgenic capacity (Igri). Microspore embryogenesis also showed comparatively higher genotypic (109.2) and phenotypic (109.5) coefficients of variation, heritability (99.62) and genetic advance (1206.77), indicating the pre-dominance of additive gene action in the control of this character in the material studied. Estimates of genetic parameters (PCV, GCV and heritability) for microspore embryogenesis were higher than for spontaneous doubled haploids. These results indicated that selection for androgenic capacity would be more effective than for spontaneous doubled haploids. The findings showed a negative relationship (r= -0.68) between embryogenesis and spontaneous chromosome doubling in the barley genotypes studied. All the large embryos used had high regenerability and good plantlet formation.

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Section: Discussionmentioning

confidence: 99%

Study of androgenesis and spontaneous chromosome doubling in barley ( Hordeum vulgare L.) genotypes using isolated microspore culture

Kahrizi

Mohammadi²

2009

Acta Agronomica Hungarica

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“…Embryogenesis was induced in microspores isolated at the late-uninucleate to early binucleate stage (Ferrie and Keller, 1995) using a defined media containing 13% Suc and heat stress at 32°C for 3 d, followed by incubation at 24°C. The characteristic enlargement of microspores that occurs during induction has been correlated with acquisition of embryogenic potential in many species (Hoekstra et al, 1992;Touraev et al, 1996aTouraev et al, , 1996b. Consequently, the 3, 5, and 7 d induced microspores were size selected on mesh screens to collect the microspores and/or cell clusters most advanced in embryogenic development and to discard the smaller physiologically unresponsive microspores.…”

Section: Plant Materialsmentioning

confidence: 99%

Transcript Profiling and Identification of Molecular Markers for Early Microspore Embryogenesis inBrassica napus

et al. 2007

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Isolated microspores of Brassica napus are developmentally programmed to form gametes; however, microspores can be reprogrammed through stress treatments to undergo appropriate divisions and form embryos. We are interested in the identification and isolation of factors and genes associated with the induction and establishment of embryogenesis in isolated microspores. Standard and normalized cDNA libraries, as well as subtractive cDNA libraries, were constructed from freshly isolated microspores (0 h) and microspores cultured for 3, 5, or 7 d under embryogenesis-inducing conditions. Library comparison tools were used to identify shifts in metabolism across this time course. Detailed expressed sequence tag analyses of 3 and 5 d cultures indicate that most sequences are related to pollen-specific genes. However, semiquantitative and real-time reverse transcription-polymerase chain reaction analyses at the initial stages of embryo induction also reveal expression of embryogenesis-related genes such as BABYBOOM1, LEAFY COTYLEDON1 (LEC1), and LEC2 as early as 2 to 3 d of microspore culture. Sequencing results suggest that embryogenesis is clearly established in a subset of the microspores by 7 d of culture and that this time point is optimal for isolation of embryo-specific expressed sequence tags such as ABSCISIC ACID INSENSITIVE3, ATS1, LEC1, LEC2, and FUSCA3. Following extensive polymerase chain reaction-based expression profiling, 16 genes were identified as unequivocal molecular markers for microspore embryogenesis in B. napus. These molecular marker genes also show expression during zygotic embryogenesis, underscoring the common developmental pathways that function in zygotic and gametic embryogenesis. The quantitative expression values of several of these molecular marker genes are shown to be predictive of embryogenic potential in B. napus cultivars (e

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“…Sucrose starvation, in particular, appears to be the major signal controlling the developmental fate of tobacco microspores, as it is the most effective treatment for embryogenic induction from different developmental stages, uninucleate microspores as well as mid-bicellular pollen. Starvation may also be a general trigger of pollen embryogenesis in higher plants, as it is effective not only in tobacco but also in other species such as barley (Hoekstra et al, 1992), wheat (Indrianto et al, 1999); rice (Ogawa et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

Induction of Sporophytic Division in Orchids Microspores by Stress

Indrianto¹,

Siregar

Linuhung

et al. 2015

KLS

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Orchid is one of the important ornamental plants in Indonesia this plant generally propagated by seed. Enhancing quality of this plant through breeding technology by various plant tissue culture methods and biotechnology, including doubled haploid technology are necessary. The most efficient method in creating doubled haploids plant is via microspore embryogenesis. We have develop new, innovative doubled haploid technology using the technique of isolated microspore culture. The goals are to obtain data on the male gametophyte development, viable embryogenic microspores, microspores derived embryos and double haploid plants of Orchid.Development of male gametophytes were analysed by isolation of microspores and pollen at various stages and staining with DAPI. Isolated orchid buds of Dendrobium hybrid 1, Vanda tricolor and Spathoglotis plicata were subjected to cold temperatures (4 o C) for 7 days, microspores were then isolated by crushing the pollinia using glass rod and cultured them in embryogenesis A2, NP, MS and VW medium, viability of the microspores were determine by using Flourescein diacetate (FDA). Isolated Orchid pollinia were cultured in starvation medium B at various temperatures and duration of time to evaluate embryogenic response, isolated microspores then were cultured further in the basic embryogenesis medium and incubated at 25 o C in the darkness.The result showed that floral characteristics for the late-uninucleate stage of the microspores were different for every orchid spesies. Ovulum lenght was used for Vanda, while in Dendrobium, Phalaenopsis, Arachnis, Spathoglottis plicata and Cattleya, varied length of flower bud was used. Isolated microspores of Dendrobium hybrid 1, Vanda tricolor and Spathoglotis plicata at 7 th days of culture in different media formulation showing different respond of viability. Medium A2 keeping viability of Dendrobium hybrid 1 microspores better than any other medium, while in Vanda tricolor and Spathoglotis plicata embryogenesis NP medium was superior. Incubation of orchid pollinia at 4 and 25 o C were successfully maintain viability of the microspores during starvation periods but not able to block gametophytic development. In contrast starved pollinia at 33 o C were succesfully block gametophytic development, percentage of embryogenic microspores after starvation of isolated pollinia at 33°C for 4 days was superior compare to any other treatments. Symmetrical divisions and some multicellular structures were observed, which were clear indication for the sporophytic development of microspore-derived embryos, they had developed and after a few weeks they degenerated and died.

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Anther and microspore culture of Hordeum vulgare L. cv. Igri

Cited by 130 publications

References 28 publications

Study of androgenesis and spontaneous chromosome doubling in barley ( Hordeum vulgare L.) genotypes using isolated microspore culture

Study of androgenesis and spontaneous chromosome doubling in barley ( Hordeum vulgare L.) genotypes using isolated microspore culture

Transcript Profiling and Identification of Molecular Markers for Early Microspore Embryogenesis inBrassica napus

Induction of Sporophytic Division in Orchids Microspores by Stress

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