Anther culture and androgenetic plant regeneration in buckwheat (Fagopyrum esculentum Moench)

Abstract

Anther culture for haploid induction of buckwheat was studied over a period of five years. Approximately 24,000 anthers were isolated and cultured on different culture media. The regeneration capacity was generally very low. Data are presented for experiments that included 7278 anthers on which 99 calluses were formed and 20 buds regenerations were noted. Regeneration occurred most readily on gellan-gum solidified media, with 90g1-1 maltose, 2.5 mgl -I BA, 0.5 mg1-1 IAA, and preferably in darkness. Haploid cells, as established by chromosome counts, were observed in eight regenerants. Several abnormalities of pollen development in vitro were detected. Starch presence in pollen as a possible sign of androgenic capacity was studied. Microspores in uninucleate and early binucleate stages contained only proplastids, while in adult pollen grains a number of amyloplasts were present.

Effect of growth regulators on microspore embryogenesis in coconut anthers

“…The optimum yield of embryogenic structures has required different auxins in different crop species (Zhou and Konzak 1989;Ishizaka 1998;Han et al 2000). Among the auxins, 2,4-D has been employed in many anther culture systems (Ball et al 1993) and its optimum concentration for induction of androgenesis has been species dependent (Custódio et al 2005). Short-term exposure to high 2,4-D concentrations followed by reduced levels was more effective in wheat (Zheng and Konzak 1999).…”

“…Auxins have been essential for induction of callus from cultured anthers of wheat (Triticum aestivum; Ball et al 1993). The type and concentration of auxin has affected the frequency of callus yield, the proportion of albino plants regenerated from calli and the frequencies of dihaploids among green plants recovered (Ball et al 1993;Trejo-Tapia et al 2002).…”

Plantlet Regeneration via Somatic Embryogenesis from Hypocotyls of Common Buckwheat (Fagopyrum esculentum Moench.)

“…However, in our experiments, direct regeneration of shoots also was observed occasionally. In vitro regeneration of cultivated buckwheat has been reported from a range of explant sources including: hypocotyls (Lachmann and Adachi, 1990;Woo et al, 1997;Yamane, 1974), cotyledons (Miljus-Djukic et al, 1992;Srejovic and Neskovic 1981;Woo et al, 2000), immature inflorescences (Takahata, 1988), protoplasts (Adachi et al, 1989), and anthers (Bohanec et al, 1993). Somatic embryogenesis has occurred in cultures of immature embryos of common buckwheat (Lachmann and Adachi, 1990).…”

Evaluation of different approaches to buckwheat (Fagopyrum esculentum Moench.) micropropagation

“…Jin et al (2002) used NAA (1-naphthalene acetic acid) with IBA for root initiation. Other reports described the addition of only IBA to the media to improve rooting (Nešković et al 1987;Srejović & Nešković 1981;Bohanec et al 1993;Miljuš-Djukić et al 1992). 41.4%-62.5% of plants were successfully transferred to the soil.…”