Evaluation of different approaches to buckwheat (Fagopyrum esculentum Moench.) micropropagation

Klčová, Lenka; Gubišová, Marcela

doi:10.17221/2677-cjgpb

Cited by 5 publications

(1 citation statement)

References 11 publications

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“…All these suggested that 6-BA was necessary for buckwheat somatic embryogenesis. Other research also suggested that regeneration from nodal segments on MS medium with 1.0 mgL −1 BA solidified by agar was the efficient technique of buckwheat micropropagation and in vitro maintenance [27]. Another research also indicated that treatment with BAP significantly induced shoot regeneration from cotyledon cultures of buckwheat [28].…”

Section: Discussionmentioning

confidence: 99%

Somatic Embryogenesis and Plant Regeneration from Cotyledon and Hypocotyl Explants of Fagopyrum esculentum Moench lpls Mutant

et al. 2019

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Buckwheat (Fagopyrum esculentum, Family Polygonaceae) is an annual pseudo-cereal crop with healing benefits. However, the genetic improvement of common buckwheat has achieved only limited success, mainly due to buckwheat’s dimorphic flowers and heteromorphic self-incompatibility. Here, we develop a useful protocol for indirect somatic embryogenesis and subsequent plant regeneration from hypocotyl explants of F. esculentum. Firstly, the initial calli of hypocotyl explants were induced on Murashige and Skoog (MS) basal medium containing 2.0 mgL−1 2,4-D and 1.5 mgL−1 6-BA for 30 days culture, and then the yellowish white friable embryogenic calli were developed when the initial calli were transferred to fresh MS basal medium supplemented with 1.0 mgL−1 6-BA and 0.5 mgL−1 thidiazuron (TDZ)two to three times subculture at 40–60 days intervals. Subsequently, the somatic embryos were able to germinate from embryogenic callus sub-cultured on MS basal medium containing 1.0 mgL−1 6-BA and 0.5 mgL−1 TDZ with 15% potato puree for 20 days subculture. Finally, maximum mean percentage (75.75%) of somatic embryo-derived plants were obtained when the mature somatic embryos were transferred to MS basal medium without growth regulators for 40 days culture. Our result provides a useful protocol for plant regeneration and SE from hypocotyl explants of F. esculentum.

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Section: Discussionmentioning

confidence: 99%

Somatic Embryogenesis and Plant Regeneration from Cotyledon and Hypocotyl Explants of Fagopyrum esculentum Moench lpls Mutant

et al. 2019

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Buckwheat Tissue Cultures and Genetic Transformation

Suvorova¹

2016

Molecular Breeding and Nutritional Aspects of Buckwheat

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Using plant extracts for the micropropagation of buckwheat

Borovaya,

Boginskaya,

Klykov

2023

Ovoŝi Rossii

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Background. Various plant hormones are used (cytokinins, auxins) to increase the regeneration efficiency and the net reproduction rate of buckwheat in vitro. However, the growth and development rates of plantlets have been noted to be low under these conditions. For this reason, search for the plant extracts that are able to stimulate the regenerative ability of plants is a promising direction of biotechnological research.Materials and methods. Aseptic single-node cuttings of common buckwheat plantlets (varieties Dikul and Izumrud) were grown on MS nutrient media with plant extracts from Fagopyrum esculentum and Reynoutria japonica (0.1, 0.5, and 1%) for 21 days. The following morphobiological paramaters of the plantlets were evaluated: plant height, the number of internodes, the number of leaves, leaf length, and the number and length of roots.Results. Dealcoholized aqueous solutions of the extracts from F. esculentum and R. japonica in the studied concentrations (0.1-1%) significantly stimulated the growth and development of the buckwheat plantlets increasing their net reproduction rate (4.00-6.00) and rhizogenesis. The media with the plant extracts in concentrations of 0.1-0.5% were observed to produce the strongest positive effect. As the result, the morphobiological characteristics of the plantlets and the success rate of the micropropagation were the highest.

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Evaluation of different approaches to buckwheat (Fagopyrum esculentum Moench.) micropropagation

Cited by 5 publications

References 11 publications

Somatic Embryogenesis and Plant Regeneration from Cotyledon and Hypocotyl Explants of Fagopyrum esculentum Moench lpls Mutant

Somatic Embryogenesis and Plant Regeneration from Cotyledon and Hypocotyl Explants of Fagopyrum esculentum Moench lpls Mutant

Buckwheat Tissue Cultures and Genetic Transformation

Using plant extracts for the micropropagation of buckwheat

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