Anther Culture Effectiveness in Producing Doubled Haploids of Cereals/ Putekðòu Kultûras Efektivitâte Graudaugu Dubultoto Haploîdu Izveidoðanâ

Grauda, Dace; Miķelsone, Andra; Ļisina, Nadežda; Žagata, Kârlis; Ornicāns, Reinis; Fokina, Oksana; Lapiņa, Lita; Rashal, Isaak

doi:10.2478/prolas-2014-0016

Cited by 11 publications

(5 citation statements)

References 27 publications

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“…Furthermore, some research reported values higher than 100 green plantlets/100 anthers such as; (BROUGHTON, 2011;LANTOS et al, 2013;CASTILLO et al, 2015). In this investigation, the overall mean was 9.76 green plantlets/100 anthers, which exceeded the means of green plantlets in previously published winter wheat breeding programs (HOLME et al, 1999;TUVESSON et al, 2000;KONDIC-SPIKA et al, 2008;EL-HENNAWY et al, 2011;LANTOS et al, 2013;GRAUDA et al, 2014), where the means ranged between 0.4 and 5.8 green plantlets/100 anthers.…”

Section: Percentage Of Acclimatized Plantlets Among Ac-derived Transplanted Plantletscontrasting

confidence: 49%

Androgenic responses of winter wheat (Triticum aestivum L.) combinations in in vitro anther culture

et al. 2020

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The androgenic parameters were investigated by in vitro anther culture (AC) on nine F2-5 breeding combinations of winter wheat (Triticum aestivium L.). Each combination produced embryo-like structures (ELS), green plantlets, albino plantlets, transplanted plantlets and acclimatized plantlets, with respect to the number of anthers in AC. The number of AC-derived ELS was between 11.73 and 52.76 ELS/100 anthers with the mean of 26.22 ELS/100 anthers, out of which the number of regenerated green plantlets varied from 3.20 to 26.40 green plantlets/100 anthers and the mean was 9.76 green plantlets/100 anthers, while the number of transplanted plantlets ranged from 2.16 to 21.77 transplanted plantlets/100 anthers. Furthermore, the number of albinos/100 anthers was mitigated and varied between 0.72 and 6.20 albinos/100 anthers. We also studied the number of green and albino plantlets per 100 ELS. The rate of green plantlets per 100 ELS ranged between 14.81% and 64.01%, with the overall mean 33.59%, while the rate of albinos per 100 ELS ranged from 4.82% to 20.70% with the overall mean 11.93%. In our experiment, the rate of acclimatized plantlets (70.15-91.57%) depended mostly on the combination. This study asserted the importance of AC method in wheat for in vitro production of green plants. Although albinism was found in each combination, it did not hinder the production of green plantlets. The satisfying results were achieved in green plantlets production compared to the previously published data, but further improvement will be needed continuously, experiment by experiment. The generated acclimatized plantlets will be used in the wheat breeding program as doubled haploid (DH) lines.

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Section: Percentage Of Acclimatized Plantlets Among Ac-derived Transplanted Plantletscontrasting

confidence: 49%

Androgenic responses of winter wheat (Triticum aestivum L.) combinations in in vitro anther culture

et al. 2020

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“…DH generation techniques have been of great value to breeders, by speeding up cultivar development (Forster et al 2007;Germanà 2011) as well as to researchers by creating ideal material for transformation or genetic mapping (Kumlehn et al 2006;Poland et al 2012). The usefulness of androgenesis as the means of DHs production depends on reproducibility and efficiency, preferably independent of genetic constitution of the donor plants stocks (Ferrie and Caswell 2011;Grauda et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Arabinogalactan proteins improve plant regeneration in barley (Hordeum vulgare L.) anther culture

Makowska

Kaluzniak

Oleszczuk

et al. 2017

Plant Cell Tiss Organ Cult

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“…Some findings recorded maximum values between 25-37 green plantlets/100 anthers (Trottier et al, 1993;Navarro-Alvarez et al, 1994;Lantos et al, 2013;Weigt et al, 2020). The total average of the production of green plantlets/100 anthers, which is between 0.40 and 9.76 green plantlets/100 anthers depending on the used protocol was recorded from previous winter wheat breeding programs by Masojc et al (1993); Holme et al (1999); Tuvesson et al (2000); Kondic-Spika et al (2008); El-Hennawy et al (2011); Grauda et al (2014); Weigt et al (2019Weigt et al ( , 2020; Kanbar et al (2020aKanbar et al ( , 2020b.…”

mentioning

confidence: 87%

“…Their results showed that the spring genotypes achieved a higher number of embryo-like structures and green plantlets. Grauda et al (2014) investigated the androgenetic induction of sixteen winter wheat hybrids and five spring hybrids by anther culture method. They asserted that the spring wheat hybrids produced higher embryogenesis than the winter wheat hybrids, but the winter wheat hybrids had higher green plantlet regeneration than the spring wheat hybrids.…”

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confidence: 99%

In vitro anther culture as efficiently applied technique for doubled haploid production of wheat (Triticum aestivum L.)

Zuhair¹,

Lantos²,

Pauk³

2021

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In vitro anther culture is the most commonly-used technology for doubled haploid production in wheat (Triticum aestivum L.) and various cereals. The technology application allows the genetic attainment of homozygous pure lines from heterozygous breeding material in a single generation, and has become a rapid alternative to the conventional breeding methods. This technology also contributes to more accurately assess QTL × environmental interactions and has been applied in: genetic researches for marker-trait association study, genomics and as a target for transformation, genetic engineering, gene mapping, and mapping of quantitative trait loci (QTLs). This paper reviews the most important factors that limit the efficiency of winter wheat (Triticum aestivum L.) anther culture, such as genotype dependency and albinism, in addition to other affecting factors including the collection time of tillers and physiological growth environments of plants. Factors that improve the efficiency of wheat anther culture in mitigating both genotypic dependency and albinism have been included in this review, too, for example, genetic improvements by following a strategy to realize this purpose through using responsive breeding material for crossing, application of convenient stress pre-treatments (cold pre-treatments, heat shock treatment, colchicine, hormones, and chemicals) to induce androgenesis, and improvement of the composition of anther culture media and culture conditions. We have also highlighted the methods of chromosome doubling for the haploid wheat produced by anther culture androgenesis, such as spontaneous chromosome doubling or use of colchicine and anti-mitotic chemicals at an early stage of anther culture or as root immersion treatment.

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Anther Culture Effectiveness in Producing Doubled Haploids of Cereals/ Putekðòu Kultûras Efektivitâte Graudaugu Dubultoto Haploîdu Izveidoðanâ

Cited by 11 publications

References 27 publications

Androgenic responses of winter wheat (Triticum aestivum L.) combinations in in vitro anther culture

Androgenic responses of winter wheat (Triticum aestivum L.) combinations in in vitro anther culture

Arabinogalactan proteins improve plant regeneration in barley (Hordeum vulgare L.) anther culture

In vitro anther culture as efficiently applied technique for doubled haploid production of wheat (Triticum aestivum L.)

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