Antheraea pernyi silk sericin mediating biomimetic nucleation and growth of hydroxylapatite crystals promoting bone matrix formation

Abstract

Bone biomineralization is well-regulated processes mediated by extracellular matrix proteins. The materials that can direct nucleation of hydroxylapatite (HAp) crystals and assembly of well-structured material-minerals complex are the key to mimicking the natural mineralization. This study used sericin from Antheraea pernyi (A.pernyi), non-mulberry silkworm cocoon as template to mediate nucleation of HAp crystals. Here we find out that AS (Antheraea pernyi sericin) can nucleate the formation HAp crystals in simulated body fluid verified by XRD and FTIR observations. The HAp crystals are organized into nano-rods oriented with c-axis preferentially parallel to the long axis of AS due to hydrogen bonds and electrostatic interaction and finally aggregated into HAp globule. The cell culture of human bone marrow-derived mesenchymal stem cells (BMSCs) showed that the HAp crystals mediated by AS not only stimulate cell adhesion and proliferation but also promote 0f osteogenic differentiation, suggesting that the resultant mineralized AS biomaterial has potential in promoting bone formation. Thus our work will provide significant implication on biomineralization of A. pernyi silk sericin as a potential scaffold for tissue engineering.

Chinese Oak Tasar Silkworm Antheraea pernyi Silk Proteins: Current Strategies and Future Perspectives for Biomedical Applications

“…Furthermore, the follow‐up applications include the degradable and flexible electronics . Ap silk sericin solution, obtained after autoclave method (121 °C for 30 min), can be used directly or cast into films . The controllable degradability and biocompatibility of silk sericin materials with underlying substrates can yield novel functional systems.…”

“…Ap sericin is envisioned as a functional template for HAp mineralization . Ap‐sericin/HAp biomaterials not only stimulated the growth of human bone marrow‐derived stem cells (hBMSCs), but also facilitated the osteogenic differentiation and bone mineralization .…”

Protein-Based Hydroxyapatite Materials: Tuning Composition toward Biomedical Applications

“…In refs , , and , it was shown that sericin can induce the formation of spherical and amorphous HAp crystals in SBF. The obtained crystals exhibit poor crystallinity and preferential growth along the c -axis. , However, in the work of Takeuchi et al, it was found that the preparation conditions of sericin (extraction and storage) influenced HAp deposition in the sericin film. HAp deposition under biomimetic conditions did not occur in all sericin films, being only observed in the sericin solution with the highest content of β-sheet structure.…”

Natural Polymeric Scaffolds in Bone Regeneration

“…Also, the very same soluble sericin extracted from native silk fibers was considered in BTE for its ability to mediate the formation of HA crystals in SBF, in turn stimulating adhesion, proliferation, and osteogenic differentiation of human BM-MSCs (Yang et al, 2014;Jiayao et al, 2017).…”