Anthocyanin Composition and MybA-related Genotype in Kyoho Grape and Its Derivatives

Fan, Xiucai; Zhao, Renzong; Wang, Qianqian; Liu, Chonghuai; Fang, Jinggui

doi:10.21273/hortsci13401-18

Cited by 3 publications

(1 citation statement)

References 18 publications

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“…It positively or negatively regulates structural genes in the flavonoid pathway and maintains the balance of flavonoids in plant organs. The MdMYB1 in apple [ 52 ], PpMYB10 in peach [ 53 ] and VvMYBA1 , VvMYBA2 , VvMYBA-pa2 in grape [ 54 , 55 ] belong to the positive regulation of MYB factors and improve the transcription level of flavonoid related genes. FaMYB9/FaMYB11 [ 56 ] and FaMYB1 in strawberry [ 57 ], PpMYB18 in peach [ 58 ], Vvmybc2-l3 and Vvmybc2-l2 in grape [ 59 ], MdMYB16 and MdMYB15L in apple [ 60 , 61 ] were negative regulation of MYB factors.…”

Section: Discussionmentioning

confidence: 99%

Integrative analysis of metabolome and transcriptome profiles provides insight into the fruit pericarp pigmentation disorder caused by ‘Candidatus Liberibacter asiaticus’ infection

Wang

et al. 2021

BMC Plant Biol

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Background Mandarin ‘Shatangju’ is susceptible to Huanglongbing (HLB) and the HLB-infected fruits are small, off-flavor, and stay-green at the maturity period. To understand the relationship between pericarp color and HLB pathogen and the effect mechanism of HLB on fruit pericarp coloration, quantitative analyses of HLB bacterial pathogens and carotenoids and also the integrative analysis of metabolome and transcriptome profiles were performed in the mandarin ‘Shatangju’ variety with four different color fruits, whole green fruits (WGF), top-yellow and base-green fruits (TYBGF), whole light-yellow fruits (WLYF), and whole dark-yellow fruits (WDYF) that were infected with HLB. Results the HLB bacterial population followed the order WGF > TYBGF > WLYF > WDYF. And there were significant differences between each group of samples. Regarding the accumulation of chlorophyll and carotenoid, the chlorophyll-a content in WGF was the highest and in WDYF was the lowest. The content of chlorophyll-b in WGF was significantly higher than that in other three pericarps. There were significant differences in the total content of carotenoid between each group. WGF and TYBGF pericarps were low in phytoene, γ-carotene, β-cryptoxanthin and apocarotenal, while other kinds of carotenoids were significantly higher than those in WDYF. And WLYF was only short of apocarotenal. We comprehensively compared the transcriptome and metabolite profiles of abnormal (WGF, TYBGF and WLYF) and normal (WDYF, control) pericarps. In total, 2,880, 2,782 and 1,053 differentially expressed genes (DEGs), including 121, 117 and 43 transcription factors were identified in the three comparisons, respectively. The qRT-PCR confirmed the expression levels of genes selected from transcriptome. Additionally, a total of 77 flavonoids and other phenylpropanoid-derived metabolites were identified in the three comparisons. Most (76.65 %) showed markedly lower abundances in the three comparisons. The phenylpropanoid biosynthesis pathway was the major enrichment pathway in the integrative analysis of metabolome and transcriptome profiles. Conclusions Synthesizing the above analytical results, this study indicated that different color pericarps were associated with the reduced levels of some carotenoids and phenylpropanoids derivatives products and the down-regulation of proteins in flavonoids, phenylpropanoids derivatives biosynthesis pathway and the photosynthesis-antenna proteins.

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Section: Discussionmentioning

confidence: 99%

Integrative analysis of metabolome and transcriptome profiles provides insight into the fruit pericarp pigmentation disorder caused by ‘Candidatus Liberibacter asiaticus’ infection

Wang

et al. 2021

BMC Plant Biol

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Gret1 retrotransposon and VvmybA1 gene sequences in somatic mutants of new table grape varieties ‘Brasil’ and –Black Star’ (Vitis vinifera L.).

Strioto

Mangolin

Collet

et al. 2022

Ciência Téc. Vitiv.

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Somatic mutations in grapes are relatively frequent and associated with diversity in grape skin color and berry morphology. Mutations that occur on a side branch of the ‘Benitaka’ cultivar with rosy-red berry skin color generated the ‘Brasil’ cultivar, and mutations that occurred on a side branch of ‘Brasil’ generated the ‘Black Star’ cultivar, both showing a black color in the berry skin. Therefore, genetic characterization of the Gret1 retrotransposon and the VvmybA1 gene in ‘Italia’, ‘Rubi’, ‘Benitaka’, ‘Brasil’, and ‘Black Star’ was started to find whether the altered coloration of berries in ‘Brasil’ and ‘Black Star’ is a product of different mutation patterns in the investigated sequences. Six primer combinations were used for the amplification of different sequences of the Gret1 retrotransposon and VvmybA1 gene of the five cultivars. Polymerase chain reaction (PCR) of the Gret1 retrotransposon and the VvmybA1 gene and sequencing of the amplified products using six primer combinations showed no different alleles or different nucleotide sequences in ‘Brasil’ and ‘Black Star’. The sequencing of the VvmybA1 gene in the present study showed that the mutations that occurred in the cultivar ‘Italia’ for generating the ‘Benitaka’ cultivar persisted in the ‘Brasil’ and ‘Black Star’ cultivars.

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Cloning and Functional Analysis of NtMYB9 in ‘Jinzhanyintai’ of Narcissus tazetta var. chinensis

Fan

Chang

et al. 2022

Horticulturae

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Narcissus tazetta var. chinensis M.Roem. (Chinese Narcissus) is a traditional and famous flower in China, and its corona shows a transition from green to yellow with the opening of flowers, which is of great ornamental value. To investigate the molecular mechanism of the corona color-forming in the Chinese Narcissus, in this study, a MYB gene was screened from the transcriptome of the corona by bioinformatics analysis methods, named NtMYB9. The expression level of the NtMYB9 gene was significantly higher in the corona than in the tepal, filament, ovary and leaf, and reached the highest level at the fourth period of complete coloration of the corona. The full-length sequence of the NtMYB9 coding region was cloned using the corona cDNA as a template. Sequence analysis revealed that NtMYB9 protein contained R2 and R3 domains, phylogenetic tree analysis showed that the NtMYB9 protein was most closely related to flavonol activators. Subcellular localization showed that NtMYB9 was localized in the nucleus. The overexpression of NtMYB9 gene into tobacco leaves and the expression level of seven enzyme genes related to the flavonoid biosynthesis pathway were significantly up-regulated. The transient transformation of Petunia × hybrida (Petunia) and Phalaenopsis aphrodite (Phalaenopsis) significantly revealed that the NtMYB9 gene could turn flowers yellow. These results suggest that the NtMYB9 gene is involved in the positive regulation of flavonoid biosynthesis in the Chinese Narcissus and may promote the synthesis of flavonols. In conclusion, these findings provide a valuable resource for further studies on the regulatory mechanism of the flavonoid biosynthesis pathway, and they are also beneficial to the molecular breeding of Chinese Narcissus.

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Anthocyanin Composition and MybA-related Genotype in Kyoho Grape and Its Derivatives

Cited by 3 publications

References 18 publications

Integrative analysis of metabolome and transcriptome profiles provides insight into the fruit pericarp pigmentation disorder caused by ‘Candidatus Liberibacter asiaticus’ infection

Integrative analysis of metabolome and transcriptome profiles provides insight into the fruit pericarp pigmentation disorder caused by ‘Candidatus Liberibacter asiaticus’ infection

Gret1 retrotransposon and VvmybA1 gene sequences in somatic mutants of new table grape varieties ‘Brasil’ and –Black Star’ (Vitis vinifera L.).

Cloning and Functional Analysis of NtMYB9 in ‘Jinzhanyintai’ of Narcissus tazetta var. chinensis

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