Anthocyanin determination in blueberry extracts from various cultivars and their antiproliferative and apoptotic properties in B16-F10 metastatic murine melanoma cells

Abstract

Blueberry consumption is associated with health benefits contributing to a reduced risk for cardiovascular disease, diabetes and cancer. The aim of this study was to determine the anthocyanin profile of blueberry extracts and to evaluate their effects on B16-F10 metastatic melanoma murine cells. Seven blueberry cultivars cultivated in Romania were used. The blueberry extracts were purified over an Amberlite XAD-7 resin and a Sephadex LH-20 column, in order to obtain the anthocyanin rich fractions (ARF). The antioxidant activity of the ARF of all cultivars was evaluated by ABTS, CUPRAC and ORAC assays. High performance liquid chromatography followed by electrospray ionization mass spectrometry (HPLC-ESI-MS) was used to identify and quantify individual anthocyanins. The anthocyanin content of tested cultivars ranged from 101.88 to 195.01 mg malvidin-3-glucoside/100g fresh weight. The anthocyanin rich-fraction obtained from cultivar Torro (ARF-T) was shown to have the highest anthocyanin content and antioxidant activity, and inhibited B16-F10 melanoma murine cells proliferation at concentrations higher than 500 μg/ml. In addition, ARF-T stimulated apoptosis and increased total LDH activity in metastatic B16-F10 melanoma murine cells. These results indicate that the anthocyanins from blueberry cultivar could be used as a chemopreventive or adjuvant treatment for metastasis control.

Comparison of anti-inflammatory activities of an anthocyanin-rich fraction from Portuguese blueberries (Vaccinium corymbosum L.) and 5-aminosalicylic acid in a TNBS-induced colitis rat model

“…The ARF used presented a high content and great variety of anthocyanins, which is in agreement with previously reported findings from different cultivars around the world [26,38,39]. However, the anthocyanin profile was somewhat different, with malvidin as the major class, instead of cyanidin, and malvidin-3-galactoside and petunidin-3-arabinoside in the highest concentrations (Fig 1).…”

Purple Rice Extract Supplemented Diet Reduces DMH-Induced Aberrant Crypt Foci in the Rat Colon by Inhibition of Bacterial β-Glucuronidase

“…Both the dichloromethane and the methanolic extracts significantly inhibited colon, breast and blood cell cancers by suppressing cell growth and proliferation and also via inducing cell cycle arrest and apoptosis (Leardkamolkarn et al, 2011). In 2013, anthocyanins purified from blueberry extracts were found to inhibit melanoma tumor cell proliferation and induce apoptosis (Bunea et al, 2013). Kum doisaket contains several active compounds such as proanthocyanidin and cyanidin 3-glucoside.…”

Methyl jasmonate delays postharvest ripening and senescence in the non-climacteric eggplant ( Solanum melongena L.) fruit

“…Sudheesh et al (1997) demonstrated the beneficial, hypolipidemic effect of phenolic phytochemicals from eggplant in normal and cholesterol fed animals. Additionally, the peel of eggplant is rich in anthocyanins which play an important role in the prevention of cancer and other diseases by preventing cellular DNA damage (Bunea et al, 2013;Lim et al, 2013).…”