Anthocyanin production in a callus line of Panax sikkimensis Ban

Abstract

A root-derived callus line of Panax sikkimensis that stably accumulates anthocyanins was established by small cell aggregate selection method. The selected line showed a growth index of 221.36 and an anthocyanin content of 2.76 mg/g fw (7.076% dw) in 50-60 d of growth on a modified MS medium containing 4.5µM 2,4-dichlorophenoxy acetic acid and 1.2µM kinetin under 16-h light and 8-h dark photoperiodic conditions. Incubation under continuous light increased the growth index to 435.57 but led to a marginal dilution of anthocyanin content to 2.192 mg/g fw (6.928% dw). The purple-red pigment had absorption maximum at 528 nm. The selected callus line has shown sustained growth and productivity for more than 6 yr now. Interestingly, pigment accumulation in the selected line did not hinder the ginsenoside production in the callus tissue (0.9-1.2% fw).

Phytochemical and Morphological Evidences for Shikonin Production by Plant Cell Cultures of Onosma sericeum Willd

“…It is presumed to be related with environmental and nutritional factors. This finding also confirms the observation of Mathur et al (2010) for isolated culture of Panax sikkimensis where cultures under 16/8-h (L/D) photoperiodic conditions had less growth and produced higher anthocyanin content and in fact, incubation under continuous light increased the growth index and decreased anthocyanin content.…”

Bridelia stipularis: a new source for anthocyanin production in vitro

“…As in the present study, the high potency of 2,4-D in anthocyanins formation has been accomplished in Oxalis linearis (Meyer and van Staden 1995). The efficacy of different PGRs in anthocyanin production through callus cultures has also been reported: IAA and Kn in Daucus carota (Narayan et al 2005); 2,4-D and Kn in Panax sikkimensis (Mathur et al 2010); NAA and BA in Crataegus sinaica (Maharik et al 2009); NAA and Kn in Glehnia littoralis (Miura et al 1998). In strawberry, 2,4-D was inhibitory in the initial phase, while increase of 2,4-D and BA later enhanced the content of cyanidin-3-glucoside almost seven times greater than that of growth regulator media (Mori et al 1994).…”

“…Production of anthocyanins through in vitro strategies has been accomplished in several plants: Prunus cerasus (Blando et al 2005), Crataegus sinaica (Maharik et al 2009), Panax sikkimensis (Mathur et al 2010), and Rosa hybrida (Ram et al 2011). The identification of plant species as new sources of anthocyanins increases the pool of anthocyanin diversity.…”

“…It also opens the windows to improve its quality further and to extend its application as natural food colourants. The production of anthocyanins through plant cell culture in vitro has been accomplished in several plants species: Crataegus sinaica (Maharik et al 2009), Panax sikkimensis (Mathur et al 2010), and Rosa hybrida (Ram et al 2011). The identification of new plant species producing anthocyanins and the production of anthocyanins through their in vitro culture has great relevance.…”

In vitro morphogenic response of leaf sheath of Phyllostachys bambusoides

“…There are several studies on synthesis and accumulation of anthocyanins in calluses of different species (Mathur et al 2009). After 18 months of cultivation of the leaf sheath of P. bambusoides we verified that morphogenic response only occurred in the areas with red-purple pigmentation on the yellow-globular calluses.…”