Anthocyanin-Rich Black Currant Extract Suppresses the Growth of Human Hepatocellular Carcinoma Cells

Abstract

Dietary antioxidants, such as anthocyanins, are helpful in the prevention and control of various diseases by counteracting the imbalance of oxidative and antioxidative factors in the living systems. Black currant (Ribes nigrum L., Grossulariaceae) is known to contain high amounts of anthocyanins (250 mg/100 g fresh fruit). Black currant fruits have been used in Asian and European traditional medicine for the treatment of a variety of diseases. Black currant extract has recently been found to be the second most effective amongst nine different berry extracts studied for their free radical scavenging activity. Constituents present in black currant juice have been found to exert a number of health-promoting effects, including immunomodulatory, antimicrobial and antiinflammatory actions, inhibition of low-density lipoprotein, and reduction of cardiovascular diseases. Although antioxidant and antiinflammatory effects of black currant juice could be of value in preventing and treating oxidative stress-and inflammation-driven cancers, no experimental evidence is available to now. The objective of the present study was to evaluate the potential antiproliferative effects of black currant fruit skin extract against HepG 2 human liver cancer cells. The aqueous extract yielded an anthocyanin-rich fraction with cyanidin-3-O-rutinoside as one of the major anthocyanins. This fraction exhibited a potent cytotoxic effect on HepG 2 cells and this effect was more pronounced than that of delphinidin and cyanidin, two major aglycones of anthocyanins present in black currant. Our results indicate, for the first time, that black currant skin containing an anthocyanin-rich fraction inhibits the proliferation of liver cancer cells, possibly due to additive as well as synergistic effects. This product could be useful in the prevention and treatment of human hepatocellular carcinoma.

The Relationship between Phytochemical Composition and Biological Activities of Differently Pigmented Varieties of Berry Fruits; Comparison between Embedded in Food Matrix and Isolated Anthocyanins

“…Although plant extracts differed in their cytotoxic activity (for 72 h incubation), Cy-3-O-Glu at the same concentration as in studied extracts did not show any cytotoxicity, thus cell growth inhibition must have resulted from the exposure to other plant components or more likely their combinations. Our results are in line with the findings of Bishayee et al [35], who showed that anthocyanin-rich fraction of BCur extract exhibited a potent cytotoxic effect in HepG2 cells and this effect was more pronounced than that of delphinidin and cyanidin, two major aglycones of anthocyanins present in BCur. These results indicate that there might possibly be some additive or synergistic effects between components of BCur skin extracts.…”

Anthocyanin-rich blackcurrant extract inhibits proliferation of the MCF10A healthy human breast epithelial cell line through induction of G0/G1 arrest and apoptosis

“…Previous studies have demonstrated that polyphenols induce DNA damage, and G2/M or G0/G1 cell cycle arrest (16)(17)(18)(19). BCE has been demonstrated to exert an anti-proliferative effect on HT29 colon cancer cells, possibly through the suppression of the cyclin-dependent kinase inhibitor 1 (p21 WAF1 ) signaling pathway (20) and a potent cytotoxic effect against hepatocellular carcinoma (21). However, in the present study, microarray and Ingenuity Pathway Analysis did not indicate suppression of the p21 WAF1 signaling pathway (data not shown).…”

Natural antioxidant extracts as food preservatives [pdf]

“…Aliment., 16(4), 361-370. http://dx.doi.org/10.17306/J. AFS.2017.0530 www.food.actapol.net/ Bishayee et al (2010) demonstrated that cyanidin--3-O-rutinoside ( Fig. 1), an anthocyanin present in the extract of black gooseberry, could inhibit the growth of liver cancer HepG2 cells.…”