Anthocyanins accumulation and molecular analysis of correlated genes by metabolome and transcriptome in green and purple asparaguses (Asparagus officinalis, L.)

Dong, Tingting; Han, Rongpeng; Yu, Jiawen; Zhu, Mingku; Zhang, Yi; Gong, Ying; Li, Zongyun

doi:10.1016/j.foodchem.2018.07.120

Cited by 150 publications

(132 citation statements)

References 38 publications

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“…For testae flavonoids-metabolomics, testae lipidmetabolomics, and embryo untarget-metabolomics, freeze-dried coats were extracted with differentially buffer according to the instructions of METWARE (Wuhan, China) and BGI (Wuhan, China). Sample extracts were analyzed using an LC-ESI-MS/MS system (HPLC, Shimpack UFLC SHIMADZU CBM30A system; MS, Applied Biosystems 6500 Q TRAP; MS, API 6500 Q TRAP), and quantified as previously described [67]. The metabolite contents were scaled using Z-score of peak area (mean value of three biological replications) in the heatmap.…”

Section: Metabolomics and Fatty Acid Contentsmentioning

confidence: 99%

Transcriptome and metabolome reveal redirection of flavonoids in a white testa peanut mutant

et al. 2020

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Background: Coat color determines both appearance and nutrient quality of peanut. White seed coat in peanut can enhance the processing efficiency and quality of peanut oil. An integrative analysis of transcriptomes, metabolomes and histocytology was performed on wsc mutant and its wild type to investigate the regulatory mechanisms underlying color pigmentation.Result: Metabolomes revealed flavonoids were redirected in wsc, while multi-omics analyses of wsc mutant seeds and testae uncovered WSC influenced the flavonoids biosynthesis in testa as well as suberin formation, glycolysis, the TCA cycle and amino acid metabolism. The mutation also enhanced plant hormones synthesis and signaling. Further, co-expression analysis showed that FLS genes co-expressed with MBW complex member genes. Combining tissue expression patterns, genetic analyses, and the annotation of common DEGs for these three stages revealed that three testa specific expressed candidate genes, Araip.M7RY3, Aradu.R8PMF and Araip.MHR6K were likely responsible for the white testa phenotype. WSC might be regulated expression competition between FLS and DFR by controlling hormone synthesis and signaling as well as the MBW complex. Conclusions:The results of this study therefore provide both candidate genes and novel approaches that can be applied to improve peanut with desirable seed coat color and flavonoid quality.

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Section: Metabolomics and Fatty Acid Contentsmentioning

confidence: 99%

Transcriptome and metabolome reveal redirection of flavonoids in a white testa peanut mutant

et al. 2020

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“…Compared the concentration of oenin and malvidin-3G at G8 to G2, there are 8267-and 6147-fold increase, respectively. Because these kinds of anthocyanins have been reported in berries (Lonicera caerulea, Rubus fruticosus, Ribes nigrum and Morus alba), cereals (Zea mays) and vegetables (Brassica oleracea, Dioscorea alata, Daucus carota and Asparagus officinalis) [59][60][61], the LC-MS/MS analysis results have verified that the purple pigment yielded by A. sydowii H-1 is anthocyanins.…”

Section: Widely Targeted Flavonoid Metabolomicsmentioning

confidence: 99%

Identification of a novel anthocyanin synthesis pathway in the fungus Aspergillus sydowii H-1

Zhang

Zeng

et al. 2020

BMC Genomics

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Background: Anthocyanins are common substances with many agro-food industrial applications. However, anthocyanins are generally considered to be found only in natural plants. Our previous study isolated and purified the fungus Aspergillus sydowii H-1, which can produce purple pigments during fermentation. To understand the characteristics of this strain, a transcriptomic and metabolomic comparative analysis was performed with A. sydowii H-1 from the second and eighth days of fermentation, which confer different pigment production. Results: We found five anthocyanins with remarkably different production in A. sydowii H-1 on the eighth day of fermentation compared to the second day of fermentation. LC-MS/MS combined with other characteristics of anthocyanins suggested that the purple pigment contained anthocyanins. A total of 28 transcripts related to the anthocyanin biosynthesis pathway was identified in A. sydowii H-1, and almost all of the identified genes displayed high correlations with the metabolome. Among them, the chalcone synthase gene (CHS) and cinnamate-4hydroxylase gene (C4H) were only found using the de novo assembly method. Interestingly, the best hits of these two genes belonged to plant species. Finally, we also identified 530 lncRNAs in our datasets, and among them, three lncRNAs targeted the genes related to anthocyanin biosynthesis via cis-regulation, which provided clues for understanding the underlying mechanism of anthocyanin production in fungi. Conclusion: We first reported that anthocyanin can be produced in fungus, A. sydowii H-1. Totally, 31 candidate transcripts were identified involved in anthocyanin biosynthesis, in which CHS and C4H, known as the key genes in anthocyanin biosynthesis, were only found in strain H1, which indicated that these two genes may contribute to anthocyanins producing in H-1. This discovery expanded our knowledges of the biosynthesis of anthocyanins and provided a direction for the production of anthocyanin.

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“…Analysis of Flavonoids-targeted metabolomics in the white and purple flowers of Phalaenopsis identified 142 different flavonoid-related metabolites, of which the most important anthocyanin was the derivative of cyaniding [16]. A study on green and purple asparagus suggested that the difference in color was mainly caused by the contents of peonidin and cyanidin and their glycoside derivatives [17]. Metabolomic analysis the color difference of fig peel showed that the content of anthocyanin derivatives in purple peel was significantly higher than that in green fruit [18].…”

Section: Introductionmentioning

confidence: 99%

Comparative Metabolomic Analysis Reveals Distinct Flavonoid Biosynthesis Regulation for Leaf Color Development of Cymbidium sinense ‘Red Sun’

Gao

Ren

Wei

et al. 2020

IJMS

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The colorful leaf is an important ornamental character of Cymbidium sinense (C. sinense), especially the red leaf, which has always been attracted by breeders and consumers. However, little is documented on the formation mechanism of the red leaf of C. sinense. In this study, the changing patterns of flavonoid-related metabolites, corresponding enzyme activities and genes expression in the leaves of C. sinense ‘Red Sun’ from red to yellow and finally to green was investigated. A total of 196 flavonoid-related metabolites including 11 anthocyanins metabolites were identified using UPLC-MS/MS-based approach. In the process of leaf color change, 42 metabolites were identified as having significantly different contents and the content of 28 differential metabolites turned to zero. In anthocyanin biosynthetic pathway, content of all 15 identified metabolites showed downregulation trend in the process of leaf color change. Among the 15 metabolites, the contents of Naringenin chalcone, Pelargonidin O-acetylhexoside and Anthocyanin 3-O-beta-d-glucoside decreased to zero in the green leaf stage. The changing pattern of enzyme activity of 10 enzymes involved in the anthocyanin biosynthetic pathway showed different trends from red leaves that have turned yellow and finally green, while the expression of genes encoding these enzymes was all down-regulated in the process of leaf color change. The results of this study revealed the types of flavonoid-related metabolites and the comprehensive analysis of metabolites content, enzyme activities and genes expression providing a new reference for breeders to improve the leaf color of C. sinense ‘Red Sun’.

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Anthocyanins accumulation and molecular analysis of correlated genes by metabolome and transcriptome in green and purple asparaguses (Asparagus officinalis, L.)

Cited by 150 publications

References 38 publications

Transcriptome and metabolome reveal redirection of flavonoids in a white testa peanut mutant

Transcriptome and metabolome reveal redirection of flavonoids in a white testa peanut mutant

Identification of a novel anthocyanin synthesis pathway in the fungus Aspergillus sydowii H-1

Comparative Metabolomic Analysis Reveals Distinct Flavonoid Biosynthesis Regulation for Leaf Color Development of Cymbidium sinense ‘Red Sun’

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