Anthraquinones from in vitro root culture of Morinda royoc L.

Abstract

Morinda royoc L. (Rubiaceae) root cultures were established for the production of anthraquinones (AQs). Three independent experiments were performed to evaluate the effects of different levels of indole-3-acetic acid (0-22.8 lM), culture duration (15-75 days) and subculture number (0-4). The following indicators were recorded: root fresh weight per Erlenmeyer and intracellular and extracellular AQ production. The experiments performed in this study allowed an increase of intracellular AQ content up to a maximum of 4.5 mg g -1 of fresh mass, after 30 days of culture in a medium 5.7 lM of IAA. In addition, isolation and identification of seven AQs from M. royoc L. roots is described, one of them being reported for the first time for this species. The structures of isolated compounds were determined from 1 H-NMR data. To the best of our knowledge, this is the first report on AQ production from root culture of this plant.

Antimicrobial Activity of the Dichloromethane Extract from in vitro Cultured Roots of Morinda royoc and Its Main Constituents

“…The roots were extracted with dichloromethane (150 mL) for 72 h in the dark. The anthraquinones were obtained as reported previously [1].…”

“…This supplement is produced from ethanolic extracts of M. royoc roots. Recently, we reported the isolation and characterization of seven known anthraquinones from the dichloromethane extract of in vitro cultured roots [1]. Anthraquinones are an important group of secondary metabolites, some of which have been reported to exhibit interesting biological activities in vitro, such as: anti-bacterial, anti-fungal, anti-tuberculosis, anti-cancer, anti-viral, and anti-oxidant [2].…”

Establishment of adventitious root culture of Stevia rebaudiana Bertoni in a roller bottle system

“…The development of biotechnological methods, such as micropropagation, cell/root and hairy root cultures, is one of the solutions to overcome this problem (Borroto et al 2008). There are numerous examples of tissue culture based on micropropagation and phytochemical productions from S. rebaudiana leaves, but there are few reports on the establishment of hairy root cultures (Yamazaki and Flores 1991).…”

“…The development of an adventitious root culture system for S. rebaudiana offers unique opportunities for the production of drugs, without having recourse to field cultivation. Moreover, root culture is an alternative method of both clonal propagation and germoplasm conservation (Borroto et al 2008;Frankfater et al 2009;Bernabé-Antonio et al 2010).…”

Damnacanthal from the Congolese Medicinal Plant Garcinia huillensis has a Potent Preferential Cytotoxicity against Human Pancreatic Cancer PANC‐1 Cells

“…Their structures were elucidated by analysis of spectroscopic data and comparison with data from the literature. The compounds were identified as damnacanthal ( 1 ) (Lin et al ., ), rubiadin dimethyl ether ( 2 ) (Siddiqui et al ., ), nordamacanthal ( 3 ) (Borroto et al ., ), rubiadin 1‐methyl ether ( 4 ) (Borroto et al ., ; Likhitwitayawuid et al ., ), lucidin ( 5 ) (Borroto et al ., ; Zhang et al ., ), lucidin 1,3‐dimethyl ether ( 6 ) (Fraga et al ., ), rubiadin ( 7 ) (Borroto et al ., ; Likhitwitayawuid et al ., ), tectoquinone ( 8 ) (Cheng et al ., ), damnacanthol 1‐methyl ether ( 9 ) (Zhou et al ., ), 2‐formyl‐1‐hydroxyanthraquinone ( 10 ) (Ismail et al ., ), pachybasin ( 11 ) (Liu et al ., ) and rubiadin 3‐methyl ether ( 12 ) (Lee, ) (Fig. ).…”