Anti-acids lead to immunological and morphological changes in the intestine of BALB/c mice similar to human food allergy

Pali‐Schöll, Isabella; Yildirim, Ali Önder; Ackermann, Ute; Knauer, Tanja; Becker, Christoph; Garn, Holger; Renz, Harald; Jensen‐Jarolim, Erika; Fehrenbach, Heinz

doi:10.1016/j.etp.2008.03.004

Cited by 27 publications

(23 citation statements)

References 32 publications

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“…Notably, mast cells and eosinophils accumulated in patches within the mucosa and there was a gradual decrease in these three cell types moving from the duodenum to the jejunum and ileum. Similar to our observations, a fish allergy study reported a significant increase in eosinophils in the duodenum only while eosinophil cell counts from the jejunum and ileum are low [42] . Lin et al [11] characterized immune changes in the duodenum from allergic patients that included an increase in IgE-bearing cells and infiltration of eosinophils and CD3+, CD4+ and CD8+ cells.…”

Section: Discussionsupporting

confidence: 79%

Gastrointestinal Immune Response to the Shrimp Allergen Tropomyosin: Histological and Immunological Analysis in an Animal Model of Shrimp Tropomyosin Hypersensitivity

Lam¹,

Tong²,

Kwan³

et al. 2015

Int Arch Allergy Immunol

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Background: Shellfish hypersensitivity is among the most common food allergies. A murine model of IgE-mediated shrimp allergy has been established in our laboratory. The aim of this study is to determine the intestinal histological changes and cytokine expression profile of this model sensitized with the major shellfish allergen tropomyosin. Methods: Female Balb/c mice orally sensitized and challenged with recombinant tropomyosin were sacrificed. Continuous sections of duodenum, jejunum and ileum were prepared using the Swiss roll technique for histological and immunological analysis. Duodenal epithelial cell apoptosis and migration were examined. mRNA expression of IL-4, IL-6, IL-10, IL-13, IL-18 and IFN-γ in intestinal tissue was measured via RT-PCR. Results: In tropomyosin-sensitized and challenged mice, an increased number of eosinophils, mast cells and goblet cells was found 24 h after challenge. There were also increased mast cell and goblet cell numbers at 72 h after challenge, but the level of eosinophils decreased. Differences compared with control mice are most prominent at the duodenum compared to the distal regions. In addition, TUNEL assay indicates a significantly higher apoptosis rate in sensitized mice sacrificed 72 h after challenge, and mRNA expression showed a biased Th2/Th1 cytokine profile and a higher level of murine mast cell protease 1. Conclusions: This study documented a multitude of histological and immunological changes in the gut in a murine model of shrimp allergy. Even without repetitive intragastric challenge, shrimp tropomyosin induces an increase in the number of inflammatory cells to varying degrees within the small intestine. This model provides an important tool for testing new therapeutic interventions.

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Section: Discussionsupporting

confidence: 79%

Gastrointestinal Immune Response to the Shrimp Allergen Tropomyosin: Histological and Immunological Analysis in an Animal Model of Shrimp Tropomyosin Hypersensitivity

Lam¹,

Tong²,

Kwan³

et al. 2015

Int Arch Allergy Immunol

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“…Its evaluation in blood plasma could represent a useful tool to follow the progression of atherosclerosis [12, 13]. In fact, some studies documented an increased proportion of LDL(–) in patients at high cardiovascular risk [12] and in familial hypercholesterolemia [14, 15], hypertriglyceridemia [16,17,18,19], type 1 and 2 diabetes mellitus [20,21,22,23,24,25,26] and athletes with intense aerobic activity [27]. In end-stage renal disease patients, Ziouzenkova et al [10] reported the presence of LDL(–) in hemodialysis (HD) patients.…”

Section: Introductionmentioning

confidence: 99%

Electronegative LDL and Lipid Abnormalities in Patients Undergoing Hemodialysis and Peritoneal Dialysis

et al. 2008

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Background: Oxidative modification of low-density lipoprotein (LDL) has been demonstrated in patients with end-stage renal disease, where it is associated with oxidative stress and plays a key role in the pathogenesis of atherosclerosis. In this context, the generation of minimally oxidized LDL, also called electronegative LDL [LDL(–)], has been associated with active disease, and is a detectable sign of atherogenic tendencies. The purpose of this study was to evaluate serum LDL(–) levels and anti-LDL(–) IgG autoantibodies in end-stage renal disease patients on dialysis, comparing patients on hemodialysis (HD), peritoneal dialysis (PD) and a control group. In addition, the serum lipid profile, nutritional status, biochemical data and parameters of mineral metabolism were also evaluated. Methods: The serum levels of LDL(–) and anti-LDL(–) IgG autoantibodies were measured in 25 patients undergoing HD and 11 patients undergoing PD at the Centro Integrado de Nefrologia, Rio de Janeiro, Brazil. Ten healthy subjects served as a control group. Serum levels of albumin, total cholesterol, triglycerides and lipoproteins were measured. Calculations of subjects’ body mass index and measurements of waist circumference, triceps skin fold and arm muscle area were performed. Measurements of hematocrit, serum blood urea nitrogen, creatinine, parathyroid hormone, phosphorus and calcium were taken. Results: Levels of LDL(–) were higher in HD patients (575.6 ± 233.1 µg/ml) as compared to PD patients (223.4 ± 117.5 µg/ml, p < 0.05), which in turn were higher than in the control group (54.9 ± 33.3 µg/ml, p < 0.01). The anti-LDL(–) IgG autoantibodies were increased in controls (0.36 ± 0.09 µg/ml) as compared to PD (0.28 ± 0.12 µg/ml, p < 0.001) and HD patients (0.2 ± 0.1 µg/ml, p < 0.001). The mean values of total cholesterol and LDL were considered high in the PD group, whereas the mean triceps skin fold was significantly lower in the HD group. Conclusion: Levels of LDL(–) are higher in renal patients on dialysis than in normal individuals, and are reciprocally related to IgG autoantibodies. LDL(–) may be a useful marker of oxidative stress, and this study suggests that HD patients are more susceptible to cardiovascular risk due to this condition. Moreover, autoantibodies reactive to LDL(–) may have protective effects in chronic kidney disease.

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“…Unexpectedly high incidence of persistent itching nodules and delayed hypersensitivity to Al in children were observed after the use of adsorbed vaccines (Bergfors et al 2003). While contacting allergy to Al in patients hyposensitized with Al-containing hyposensitizing extracts (Hindsén 2005), antacids led to immunological changes in the intestine of BALB/c mice similar to human food allergy (Pali-Schöll et al 2008).…”

Section: Introductionmentioning

confidence: 95%

Effects of aluminum exposure on the allergic responses and humoral immune function in rats

Zhu

Sun

et al. 2011

Biometals

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This study was conducted to assess effects of aluminum (Al) exposure on allergic responsive reactions and humoral immune function in rats. Forty male Wistar rats (5 weeks old) weighed 110-120 g were randomly allocated into four groups and were orally exposed to 0, 64.18, 128.36, and 256.72 mg/kg body weight aluminum trichloride in drinking water for 120 days. The levels of immunoglobulin (Ig) G, IgA, IgM, IgE, Complement factor (C)3, and C4 in serum were determined by ELISA and nephelometric assays at the end of experiment. The results showed that the levels of IgM, C3, and C4 were lowered, and the levels of IgG, IgA, and IgE were increased in an Al-dose dependent manner. The increased in IgE level and the decreased in C3 and C4 levels indicate that Al induces allergic responses in rats; while the increased levels in IgG and IgA and the decreased level in IgM suggest that Al disorders the humoral immune function in rats.

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Anti-acids lead to immunological and morphological changes in the intestine of BALB/c mice similar to human food allergy

Cited by 27 publications

References 32 publications

Gastrointestinal Immune Response to the Shrimp Allergen Tropomyosin: Histological and Immunological Analysis in an Animal Model of Shrimp Tropomyosin Hypersensitivity

Gastrointestinal Immune Response to the Shrimp Allergen Tropomyosin: Histological and Immunological Analysis in an Animal Model of Shrimp Tropomyosin Hypersensitivity

Electronegative LDL and Lipid Abnormalities in Patients Undergoing Hemodialysis and Peritoneal Dialysis

Effects of aluminum exposure on the allergic responses and humoral immune function in rats

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