Anti-adhesive glycoproteins in echinoderm mucus secretions

Bavington, Charles D.; Lever, Rebecca; Mulloy, Barbara; Grundy, Michelle M.; Page, Clive; Richardson, Neville V.; McKenzie, Jane

doi:10.1016/j.cbpc.2004.07.008

Cited by 54 publications

(32 citation statements)

References 39 publications

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“…In the present study, MOS supplementation increased goblet cells density and, thus, mucus production in agreement with previous studies [22] and further possibly reducing bacterial adhesion to the enterocytes, being bacterial adhesion a necessary step in microbial colonization and pathogenesis. Epithelial mucosal surfaces have a number of defence mechanisms to prevent bacterial adhesion, which include mucus secretion [15,18] and antiadhesive action of mucins [57]. In turn, increased mucus production could be responsible for improved gut integrity.…”

Section: Discussionmentioning

confidence: 99%

Reduced gut bacterial translocation in European sea bass (Dicentrarchus labrax) fed mannan oligosaccharides (MOS)

Torrecillas

Makol

Benítez‐Santana

et al. 2011

Fish & Shellfish Immunology

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Section: Discussionmentioning

confidence: 99%

Reduced gut bacterial translocation in European sea bass (Dicentrarchus labrax) fed mannan oligosaccharides (MOS)

Torrecillas

Makol

Benítez‐Santana

et al. 2011

Fish & Shellfish Immunology

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“…To test the role of individual mucus monomers in attachment to coral mucus-coated surfaces, 50 µl of the mucus preparation (buffered with 10 mM HEPES to pH 7) was incubated in each well of a polystyrene microtiter plate for 1 h, after which 40 µl of the liquid were aspirated and discarded, and the residue was dried at 30°C overnight in a microbiological hood, as in Bavington et al (2004). The inoculum was prepared by growing bacterial cultures overnight in LB or in GASW broth at 30°C, then washing them twice in filter-sterilized buffered seawater.…”

Section: Methodsmentioning

confidence: 99%

“…In control experiments, crude mucus of A. palmata also modestly inhibited biofilm formation in CFA medium (data not shown), suggesting that an unknown component of coral mucus inhibits biofilm formation in some bacteria. This is reminiscent of a study in which mucus of some echinoderms contained substances capable of inhibiting attachment by bacteria (Bavington et al 2004). …”

Section: Attachment To Coral Mucusmentioning

confidence: 99%

Catabolite regulation of enzymatic activities in a white pox pathogen and commensal bacteria during growth on mucus polymers from the coral Acropora palmata

Ritchie¹,

Teplitski²

2009

Dis. Aquat. Org.

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Colonization of host mucus surfaces is one of the first steps in the establishment of coralassociated microbial communities. Coral mucus contains a sulfated glycoprotein (in which oligosaccharide decorations are connected to the polypeptide backbone by a mannose residue) and molecules that result from its degradation. Mucus is utilized as a growth substrate by commensal and pathogenic organisms. Two representative coral commensals, Photobacterium mandapamensis and Halomonas meridiana, differed from a white pox pathogen Serratia marcescens PDL100 in the pattern with which they utilized mucus polymers of Acropora palmata. Incubation with the mucus polymer increased mannopyranosidase activity in S. marcescens, suggestive of its ability to cleave off oligosaccharide side chains. With the exception of glucosidase and N-acetyl galactosaminidase, glycosidases in S. marcescens were subject to catabolite regulation by galactose, glucose, arabinose, mannose and N-acetyl-glucosamine. In commensal P. mandapamensis, at least 10 glycosidases were modestly induced during incubation on coral mucus. Galactose, arabinose, mannose, but not glucose or N-acetyl-glucosamine had a repressive effect on glycosidases in P. mandapamensis. Incubation with the mucus polymers upregulated 3 enzymatic activities in H. meridiana; glucose and galactose appear to be the preferred carbon source in this bacterium. Although all these bacteria were capable of producing the same glycosidases, the differences in the preferred carbon sources and patterns of enzymatic activities induced during growth on the mucus polymer in the presence of these carbon sources suggest that to establish themselves within the coral mucus surface layer commensals and pathogens rely on different enzymatic activities. KEY WORDS: Coral microbiology · Mucus surface polysaccharide · Coral pathogenResale or republication not permitted without written consent of the publisher

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“…ACCEPTED MANUSCRIPT12 150, 156 and 156 proteins were identified in the 4 footprint samples, respectively (FDR at peptide level < 1.20%), while the analysis of the 4 mucus samples lead to the identification of, respectively, 559, 545, 523, and 615 proteins (FDR at peptide level <1.40%) (supplemental…”

mentioning

confidence: 99%

An integrated transcriptomic and proteomic analysis of sea star epidermal secretions identifies proteins involved in defense and adhesion

Hennebert

Leroy

Wattiez

et al. 2015

Journal of Proteomics

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Sea stars rely on epidermal secretions to cope with their benthic life. Their integument produces a mucus, which represents the first barrier against invaders; and their tube feet produce adhesive secretions to pry open mussels and attach strongly but temporarily to rocks. In this study, we combined high-throughput sequencing of expressed mRNA and mass-spectrometry-based identification of proteins to establish the first proteome of mucous and adhesive secretions from the sea star Asterias rubens. We show that the two secretions differ significantly, the major adhesive proteins being only present in trace amounts in the mucus secretion. Except for 41 proteins which were present in both secretions, a total of 34 and 244 proteins were identified as specific of adhesive secretions and mucus, respectively. We discuss the role of some of these proteins in the adhesion of sea stars as well as in their protection against oxygen reactive species and microorganisms. In addition, 58% of the proteins identified in adhesive secretions did not present significant similarity to other known proteins, revealing a list of potential novel sea star adhesive proteins uncharacterized so far. The panel of proteins identified in this study offers unprecedented opportunities for the development of sea star-inspired biomimetic materials.

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Anti-adhesive glycoproteins in echinoderm mucus secretions

Cited by 54 publications

References 39 publications

Reduced gut bacterial translocation in European sea bass (Dicentrarchus labrax) fed mannan oligosaccharides (MOS)

Reduced gut bacterial translocation in European sea bass (Dicentrarchus labrax) fed mannan oligosaccharides (MOS)

Catabolite regulation of enzymatic activities in a white pox pathogen and commensal bacteria during growth on mucus polymers from the coral Acropora palmata

An integrated transcriptomic and proteomic analysis of sea star epidermal secretions identifies proteins involved in defense and adhesion

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