Anti‐adipogenesis and metabolism‐regulating effects of heat‐inactivated Streptococcus thermophilus MN‐ZLW‐002

Abstract

Metabolic syndrome and obesity have become serious threats to public health worldwide. This study was conducted to evaluate the anti‐adipogenesis and metabolism‐regulating effects of heat‐inactivated Streptococcus thermophilus MN‐ZLW‐002 (MN‐ZLW‐002), which can be used as a yogurt starter. In vitro study suggested that MN‐ZLW‐002 stimulated the RAW264.7 macrophages to produce significant amounts of interleukin (IL)‐6, IL‐10 and tumour necrosis factor (TNF)‐α and induced intense phosphorylation of P38, p44/42 MAPK and nuclear factor κB. MN‐ZLW‐002‐stimulated RAW264.7‐conditioned medium (CM) notably suppressed the differentiation and adipogenesis of 3T3‐L1 pre‐adipocytes. The 12‐week in vivo study suggested that orally administered MN‐ZLW‐002 significantly reduced the weight gain of mice caused by the high‐fat diet (HFD) at weeks 3–8; decreased fasting blood glucose levels at week 4 and week 8; decreased serum total triglyceride level at week 12. MN‐ZLW‐002 also reduced serum IL‐1β and chemokine ligand 3 levels in the HFD‐fed mice. These findings suggest that heat‐inactivated MN‐ZLW‐002 can suppress adipocytes differentiation and lipid accumulation by regulating the immune response, possibly via the release of cytokines, particularly TNF‐α; MN‐ZLW‐002 can improve metabolism‐related indicators in the early stage of HFD intervention and regulate the related pro‐inflammatory immune response.

Influence of high-fat diet on host animal health via bile acid metabolism and benefits of oral-fed <i>Streptococcus thermophilus</i> MN-ZLW-002

“…Moreover, the acetic, propionic, and butyric acid contents of the cecum decreased, while the fecal bile acid content was significantly altered. The above results of this study agreed well with those of the previous studies [1,2,[6][7][8][9][10][11][17][18][19][20][21][22][23][24][25][26], which stated that HFDs contributed to obesity and that glucose and lipid metabolic perturbations were related to gut microbiota dysbiosis. These results further verify the health hazards of HFDs.…”

“…Therefore, S. thermophilus might be a promising probiotic strain [14,18]. Our previous research results showed that inactivated MN002 has the ability to inhibit the differentiation of preadipocytes in vitro, and in animal experiments, inactivated Streptococcus thermophilus MN-ZLW-002 was observed to reduce the weight gain of mice induced by a high-fat diet on early stage [19].However, the health benefits of live S. thermophilus on HFD-induced metabolic disorders remain unclear.…”

Seaweed-Derived Phlorotannins: A Review of Multiple Biological Roles and Action Mechanisms

“…Obesity, diabetes, dyslipidemia, myocardial infarction, stroke, and hypertension are all symptoms of metabolic syndrome [97]. Metabolic syndrome is becoming more common in modern life and is regarded as a major health issue.…”

Usefulness of Probiotics in the Management of NAFLD: Evidence and Involved Mechanisms of Action from Preclinical and Human Models

“…In comparison to probiotics (live bacteria), parabiotics represent several potential advantages, which include a lower risk of infection and antibiotic resistance acquisition/transfer, as well as an easier storage and handling [ 78 ]. Even though the available data regarding the usage of parabiotics in the management of different diseases are still scarce, it was described that administration of heat-inactivated probiotic bacteria ( Streptococcus thermophilus MN-ZLW-002) is effective in preventing high-fat diet feeding induced body weight gain, insulin resistance and dyslipidemia in mice [ 79 ]. In addition, according to data reported in clinical trials, the continuous administration of fragmented Lactobacillus amylovorus CP1563 (heat inactivated, lyophilized and then milled) for 12 weeks significantly reduces whole body and visceral fat, ameliorates markers related to glycaemic control (reduced fasting blood glucose and insulin levels) and improves dyslipidemia (reducing blood TG, and total and LDL cholesterol levels) in subjects featuring class I obesity [ 80 ].…”