Anti-adipogenic and anti-oxidant effects of a standardised extract of Moro blood oranges (<i>Citrus sinensis</i>(L.) Osbeck) during adipocyte differentiation of 3T3-L1 preadipocytes

Tomasello, Barbara; Malfa, Giuseppe Antonio; Mantia, Alfonsina La; Miceli, Natalizia; Sferrazzo, Giuseppe; Taviano, Maria Fernanda; Giacomo, Claudia Di; Renis, Marcella; Acquaviva, Rosaria

doi:10.1080/14786419.2019.1660337

Cited by 23 publications

(16 citation statements)

References 27 publications

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“…Consistently, supplementation with Moro orange extract significantly decreased the activation of both ACC and FAS enzymes, probably due to the suppression of PPARγ and C/EBPα adipogenic markers ( Tomasello et al., 2019 ).…”

Section: Resultsmentioning

confidence: 82%

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A review of the lipolytic effects and the reduction of abdominal fat from bioactive compounds and moro orange extracts

Lima¹,

Barbosa²

2021

Heliyon

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Dietary supplementation containing Citrus sinensis extract is being widely used for weight loss due to its anti-adipogenic and antioxidant effects that regulate the metabolism of fatty acids. Bioactive compounds upregulate PPARα in the liver tissue, increasing oxidation of fatty acids and improving insulin sensitivity in addition to decreasing the expression of genes involved in the synthesis of fatty acids, such as LXRα and FAS. Studies on synephrine demonstrated their ability to stimulate the development of beige adipose tissue through greater expression of UCP1 and mtTFA, contributing to an increase in thermogenesis and mitochondrial biogenesis. However, despite its widespread use to reduce abdominal fat, few scientific studies have consensually proven the effectiveness of Moro orange extract for weight loss. This literature review summarizes the current information on the pharmacological and molecular mechanisms involved in the modulation of lipid metabolism by the bioactive compounds present in Moro orange extract.

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Section: Resultsmentioning

confidence: 82%

“…These findings can be reinforced by another study that demonstrated in 3T3-L1 preadipocytes treated with Moro orange extract the promotion of anti-adipogenic and anti-oxidant effects, being useful in the neutralization and prevention of excess weight as a way to prevent severe conditions of obesity ( Tomasello et al., 2019 ).…”

Section: Resultsmentioning

confidence: 85%

A review of the lipolytic effects and the reduction of abdominal fat from bioactive compounds and moro orange extracts

Lima¹,

Barbosa²

2021

Heliyon

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“…Obesity is a state in which body fat is over-accumulated in the body, which not only affects the quality of life but is also a public health concern due to the occurrence of chronic degenerative diseases and metabolic complications. Therefore, to reduce obesity, anti-obesity studies using natural substances are attracting attention [ 33 , 34 , 35 ]. Previous studies have reported that various phytochemicals present in natural substances can prevent obesity through the inhibition of oxidative stress and inflammation [ 36 , 37 ].…”

Section: Discussionmentioning

confidence: 99%

Effects of Bacillus Subtilis-Fermented White Sword Bean Extract on Adipogenesis and Lipolysis of 3T3-L1 Adipocytes

Choi

Kim

Lee

et al. 2021

Foods

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To investigate the adipogenesis and lipolysis effects of the Bacillus subtilis-fermented white sword bean extract (FWSBE) on 3T3-L1 adipocytes, we treated 3T3-L1 preadipocytes before and after differentiation with FWSBE and measured triglyceride, free glycerol, mRNA, and protein levels. First, FWSBE reduced the cell viability of 3T3-L1 pre-adipocytes under 1000 µg/mL conditions. Triglyceride accumulation in 3T3-L1 pre-adipocytes was suppressed, and free glycerol content in mature 3T3-L1 adipocytes was increased in the FWSBE treatment groups, indicating that FWSBE has anti-obesity effects. Further, FWSBE suppressed adipogenesis in 3T3-L1 pre-adipocytes by lowering the protein levels of C/EBPα, PPARγ, and FAS and increasing the level of pACC and pAMPK. Additionally, FWSBE promoted lipolysis in mature 3T3-L1 adipocytes by increasing the transcription levels of Ppara, Acox, and Lcad and the protein levels of pHSL and ATGL. Thus, we suggest that FWSBE can be a potential dietary supplement because of its anti-obesity properties.

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“…Total RNA was isolated from 1321N1 cells using TRIzol reagent (Life Technologies, Carlsbad, CA, USA) according to the manufacturer’s instructions. The reverse transcription was carried out on 1 µg of total RNA using the QuantiTect ® Reverse Transcription Kit (Qiagen Inc., Valencia, MD, USA) [ 74 ]. The DNMT1 mRNA expression level was determined by RT-PCR (QuantiNova SYBR ® Green RT-PCR Kit) on Rotor-Gene Q 5PLEX (Qiagen Inc., Valencia, MD, USA).…”

Section: Methodsmentioning

confidence: 99%

Rapha Myr®, a Blend of Sulforaphane and Myrosinase, Exerts Antitumor and Anoikis-Sensitizing Effects on Human Astrocytoma Cells Modulating Sirtuins and DNA Methylation

Tomasello

Mauro

Malfa

et al. 2020

IJMS

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Brain and other nervous system cancers are the 10th leading cause of death worldwide. Genome instability, cell cycle deregulation, epigenetic mechanisms, cytoarchitecture disassembly, redox homeostasis as well as apoptosis are involved in carcinogenesis. A diet rich in fruits and vegetables is inversely related with the risk of developing cancer. Several studies report that cruciferous vegetables exhibited antiproliferative effects due to the multi-pharmacological functions of their secondary metabolites such as isothiocyanate sulforaphane deriving from the enzymatic hydrolysis of glucosinolates. We treated human astrocytoma 1321N1 cells for 24 h with different concentrations (0.5, 1.25 and 2.5% v/v) of sulforaphane plus active myrosinase (Rapha Myr®) aqueous extract (10 mg/mL). Cell viability, DNA fragmentation, PARP-1 and γH2AX expression were examined to evaluate genotoxic effects of the treatment. Cell cycle progression, p53 and p21 expression, apoptosis, cytoskeleton morphology and cell migration were also investigated. In addition, global DNA methylation, DNMT1 mRNA levels and nuclear/mitochondrial sirtuins were studied as epigenetic biomarkers. Rapha Myr® exhibited low antioxidant capability and exerted antiproliferative and genotoxic effects on 1321N1 cells by blocking the cell cycle, disarranging cytoskeleton structure and focal adhesions, decreasing the integrin α5 expression, renewing anoikis and modulating some important epigenetic pathways independently of the cellular p53 status. In addition, Rapha Myr® suppresses the expression of the oncogenic p53 mutant protein. These findings promote Rapha Myr® as a promising chemotherapeutic agent for integrated cancer therapy of human astrocytoma.

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Anti-adipogenic and anti-oxidant effects of a standardised extract of Moro blood oranges (Citrus sinensis(L.) Osbeck) during adipocyte differentiation of 3T3-L1 preadipocytes

Cited by 23 publications

References 27 publications

A review of the lipolytic effects and the reduction of abdominal fat from bioactive compounds and moro orange extracts

A review of the lipolytic effects and the reduction of abdominal fat from bioactive compounds and moro orange extracts

Effects of Bacillus Subtilis-Fermented White Sword Bean Extract on Adipogenesis and Lipolysis of 3T3-L1 Adipocytes

Rapha Myr®, a Blend of Sulforaphane and Myrosinase, Exerts Antitumor and Anoikis-Sensitizing Effects on Human Astrocytoma Cells Modulating Sirtuins and DNA Methylation

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