Anti-ageing properties of Khelma Longevity™: treatment of human fibroblasts increases proteasome levels and decreases the levels of oxidized proteins

Voutetakis, Konstantinos; Delitsikou, Vasiliki; Magouritsas, Michel Georges; Gonos, Efstathios S.

doi:10.1016/j.nbt.2017.03.002

Cited by 2 publications

(3 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Enhancing proteasome activity reduces the proteotoxic burden cells experience upon aging. In humans, , rodents, ,, and cells, ,− increased proteasome activity delays aging ,− and results in longer lifespan − by reducing proteotoxic pathologies. Supporting this idea, cells from human centenarians exhibit enhanced proteasome activity compared to cells from adults of different ages …”

Section: Challenges and Considerationsmentioning

confidence: 99%

Proteasome Activation as a New Therapeutic Approach To Target Proteotoxic Disorders

Njomen

Tepe

2019

J. Med. Chem.

View full text Add to dashboard Cite

Proteasomes are multienzyme complexes that maintain protein homeostasis (proteostasis) and important cellular functions through the degradation of misfolded, redundant, and damaged proteins. It is well established that aging is associated with the accumulation of damaged and misfolded proteins. This phenomenon is paralleled by declined proteasome activity. When the accumulation of redundant proteins exceed degradation, undesirable signaling and/or aggregation occurs and are the hallmarks of neurodegenerative diseases and many cancers. Thus, increasing proteasome activity has been recognized as a new approach to delay the onset or ameliorate the symptoms of neurodegenerative and other proteotoxic disorders. Enhancement of proteasome activity has many therapeutic potentials but is still a relatively unexplored field. In this perspective, we review current approaches, genetic manipulation, posttranslational modification, and small molecule proteasome agonists used to increase proteasome activity, challenges facing the field, and applications beyond aging and neurodegenerative diseases.

show abstract

Section: Challenges and Considerationsmentioning

confidence: 99%

Proteasome Activation as a New Therapeutic Approach To Target Proteotoxic Disorders

Njomen

Tepe

2019

J. Med. Chem.

View full text Add to dashboard Cite

show abstract

“…However, to further assess the biological activity of the administered supplement, we investigated the interrelation between levels of oxidized proteins and measured 20S proteasome levels, as the latter represents the key player in the selective removal of oxidized proteins [49]. Previous studies have established in cellulo and in vivo the inverse relationship between proteasome levels and oxidized/carbonylated proteins in an effective proteostasis [5][6][7]. As assessed in cellulo, but as also reported in mammalian systems, proteasomes are highly responsive complexes that participate in multiple regulatory networks [50].…”

Section: Discussionmentioning

confidence: 99%

“…Specifically, the proteasome is a multi-subunit protease complex, composed of the catalytic 20S "core" and the regulatory 19S "lid" that selectively catalyze the degradation of shortlived regulatory proteins and oxidized or damaged peptides [3,4]. Previous studies from our laboratory have established the inverse relationship between proteasome levels and oxidized proteins, both in vitro and in vivo [5][6][7]. Moreover, we have shown that a functional proteasome is linked to the extreme longevity seen in centenarian populations [8] and is associated with reduced frailty in the elderly [6].…”

Section: Introductionmentioning

confidence: 99%

Antioxidant and Antiaging Properties of a Novel Synergistic Nutraceutical Complex: Readouts from an In Cellulo Study and an In Vivo Prospective, Randomized Trial

Athanasopoulou

Καπετάνου

Magouritsas³

et al. 2022

Antioxidants

Self Cite

View full text Add to dashboard Cite

Aging is a dynamic procedure that is developed in multiple layers and characterized by distinct hallmarks. The use of biomarkers that target different hallmarks of aging is substantial in predicting adverse outcomes during the aging process, implementing specifically designed antiaging interventions and monitoring responses to these interventions. The present study aimed to develop a novel composition of plant extracts, comprising identified active ingredients that synergistically target different hallmarks of aging in cellulo and in vivo. The selected single extracts and the developed composition were tested through a powerful set of biomarkers that we have previously identified and studied. The composition of selected extracts simultaneously increased cellular lifespan, reduced the cellular oxidative load and enhanced antioxidant defense mechanisms by increasing proteasome activity and content. In addition, the combination prevented telomere attrition and preserved optimum DNA methylation levels. Remarkably, biomarker profiling of healthy volunteers who received the identified combination in the form of a nutritional supplement within the frame of a prospective, randomized, controlled 3-month trial revealed an unprecedented antioxidant capacity in humans. In conclusion, our results support the notion that interventions with specifically designed combinations of natural compounds targeting multiple hallmarks of aging represent an effective way to improve healthspan and well-being.

show abstract

Anti-ageing properties of Khelma Longevity™: treatment of human fibroblasts increases proteasome levels and decreases the levels of oxidized proteins

Cited by 2 publications

References 20 publications

Proteasome Activation as a New Therapeutic Approach To Target Proteotoxic Disorders

Proteasome Activation as a New Therapeutic Approach To Target Proteotoxic Disorders

Antioxidant and Antiaging Properties of a Novel Synergistic Nutraceutical Complex: Readouts from an In Cellulo Study and an In Vivo Prospective, Randomized Trial

Contact Info

Product

Resources

About